##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062812_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 664113 Sequences flagged as poor quality 0 Sequence length 38 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.148283499946544 32.0 32.0 32.0 32.0 32.0 2 30.83247429277849 32.0 32.0 32.0 32.0 32.0 3 30.895430446324646 32.0 32.0 32.0 32.0 32.0 4 30.941625898002297 32.0 32.0 32.0 32.0 32.0 5 30.89510369470256 32.0 32.0 32.0 32.0 32.0 6 34.53411091184783 36.0 36.0 36.0 32.0 36.0 7 34.44143240683438 36.0 36.0 36.0 32.0 36.0 8 34.416371912611254 36.0 36.0 36.0 32.0 36.0 9 34.527414762246785 36.0 36.0 36.0 32.0 36.0 10 34.29581110443554 36.0 36.0 36.0 32.0 36.0 11 34.55809929936622 36.0 36.0 36.0 32.0 36.0 12 34.395765479669876 36.0 36.0 36.0 32.0 36.0 13 34.46778635563526 36.0 36.0 36.0 32.0 36.0 14 34.368959800515874 36.0 36.0 36.0 32.0 36.0 15 34.346228729147 36.0 36.0 36.0 32.0 36.0 16 34.34314642237089 36.0 36.0 36.0 32.0 36.0 17 34.27853542996448 36.0 36.0 36.0 32.0 36.0 18 34.31681054278413 36.0 36.0 36.0 32.0 36.0 19 34.28414742671804 36.0 36.0 36.0 32.0 36.0 20 34.24110354713731 36.0 36.0 36.0 32.0 36.0 21 34.22887520647841 36.0 36.0 36.0 32.0 36.0 22 34.214397248660994 36.0 36.0 36.0 32.0 36.0 23 34.152716480478475 36.0 36.0 36.0 32.0 36.0 24 34.14334909872266 36.0 36.0 36.0 32.0 36.0 25 34.122994129011175 36.0 36.0 36.0 32.0 36.0 26 34.08530325411489 36.0 36.0 36.0 32.0 36.0 27 34.086789446976645 36.0 36.0 36.0 32.0 36.0 28 34.07067773104878 36.0 36.0 36.0 32.0 36.0 29 34.031042909866244 36.0 36.0 36.0 32.0 36.0 30 33.99142314636214 36.0 36.0 36.0 32.0 36.0 31 34.01864441744101 36.0 36.0 36.0 32.0 36.0 32 33.95884284752746 36.0 36.0 36.0 32.0 36.0 33 33.90500261250721 36.0 36.0 36.0 32.0 36.0 34 33.89327870407596 36.0 36.0 36.0 32.0 36.0 35 33.83863137749148 36.0 36.0 36.0 32.0 36.0 36 33.79812019942389 36.0 36.0 36.0 32.0 36.0 37 33.78772889553434 36.0 36.0 36.0 32.0 36.0 38 33.17356082473916 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 0.0 9 2.0 10 4.0 11 13.0 12 0.0 13 3.0 14 166.0 15 299.0 16 357.0 17 411.0 18 556.0 19 692.0 20 882.0 21 1126.0 22 1559.0 23 2227.0 24 3175.0 25 4589.0 26 6557.0 27 8968.0 28 12750.0 29 16981.0 30 23053.0 31 30688.0 32 41365.0 33 61729.0 34 138427.0 35 307531.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.99889641364587 17.24338602003341 11.688517274443232 25.069200291877486 2 15.98991195559814 20.473637903084867 38.261198392180255 25.27525174913673 3 18.793889933872375 25.25966877453808 28.76544195271521 27.18099933887434 4 12.233942432935047 16.088662013717915 36.44945376790971 35.227941785437324 5 14.045242436881786 37.258530576966805 33.669220958544585 15.027006027606832 6 32.6311414808669 36.132664569627664 17.59555255070312 13.640641398802309 7 29.037979982322287 31.43727046451432 21.370911275641344 18.15383827752205 8 27.832395488103646 32.98770838007501 19.98747197381161 19.19242415800973 9 27.888000481847886 14.34626793754047 18.713616720121667 39.05211486048998 10 15.575455234005261 27.440073843299817 32.21127857023471 24.773192352460214 11 36.51950007528987 21.78406866435778 22.48953470862822 19.206896551724135 12 24.440592926323905 24.491188021009012 29.43806317158965 21.630155881077435 13 29.708654231335707 20.218306019981267 25.443434103086947 24.62960564559608 14 23.791625066071536 20.02159466187187 26.09731452158186 30.089465750474737 15 24.995933465470603 26.94109248203194 23.913783443481197 24.149190609016262 16 24.76537840638026 26.407127195624412 24.833596366527722 23.993898031467605 17 23.140641496191897 26.11980198168918 26.2765840485919 24.462972473527024 18 24.189030872693433 25.27714950864502 27.43983423733816 23.09398538132339 19 24.75010164591082 25.713855467044137 26.72519463309591 22.810848253949132 20 25.17403750446105 24.494150562958435 26.698399743404792 23.63341218917572 21 26.36992128853692 24.693594510225562 25.4430625640933 23.49342163714422 22 24.926550643538338 24.837402475367547 26.121768567138957 24.11427831395515 23 23.845443743853938 24.8193264592805 26.461840573577312 24.87338922328825 24 23.825644856210015 25.940628651976432 26.181583798175968 24.052142693637578 25 24.464012481777328 24.83208033638149 26.188389296514497 24.51551788532668 26 23.635172481306054 25.784317416809117 26.767773368374286 23.812736733510544 27 24.391988555078683 25.477147805135154 26.209773360439726 23.921090279346437 28 23.794157020474973 25.318523850325036 26.810816183014367 24.07650294618562 29 23.478245155383142 25.748603991349867 26.914052852556186 23.859098000710805 30 23.928684365071 25.72211597825596 26.861268803927178 23.487930852745865 31 23.768012058987846 25.762081952327243 26.00467722480646 24.465228763878454 32 23.54812867240699 26.002222643861977 26.001770886979465 24.447877796751566 33 23.517125582586043 25.511809837892375 26.185087303200742 24.785977276320843 34 23.923539368821796 25.93218960646238 26.496171848175077 23.64809917654075 35 24.605878242430816 25.632974301514437 26.483415862459488 23.27773159359526 36 23.661236666581328 26.4561531383513 25.85888826809753 24.023721926969838 37 24.47598466994451 26.261077772172488 25.990919426854703 23.272018131028304 38 23.61265043549495 26.22637304388681 26.20227927092243 23.958697249695817 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 626.0 1 478.0 2 330.0 3 330.0 4 689.0 5 1048.0 6 1048.0 7 1239.5 8 1431.0 9 1371.0 10 1311.0 11 1311.0 12 1590.5 13 1870.0 14 2247.0 15 2624.0 16 2624.0 17 3860.5 18 5097.0 19 5097.0 20 5909.0 21 6721.0 22 6715.0 23 6709.0 24 6709.0 25 7556.0 26 8403.0 27 8403.0 28 10707.0 29 13011.0 30 15543.0 31 18075.0 32 18075.0 33 21236.0 34 24397.0 35 24397.0 36 27033.0 37 29669.0 38 33764.0 39 37859.0 40 37859.0 41 40883.5 42 43908.0 43 48763.0 44 53618.0 45 53618.0 46 57054.5 47 60491.0 48 60491.0 49 61131.5 50 61772.0 51 60459.5 52 59147.0 53 59147.0 54 56062.5 55 52978.0 56 52978.0 57 50580.5 58 48183.0 59 43077.5 60 37972.0 61 37972.0 62 34878.0 63 31784.0 64 26222.0 65 20660.0 66 20660.0 67 16987.0 68 13314.0 69 13314.0 70 10776.5 71 8239.0 72 6441.0 73 4643.0 74 4643.0 75 3599.0 76 2555.0 77 2555.0 78 2284.5 79 2014.0 80 1594.5 81 1175.0 82 1175.0 83 1191.5 84 1208.0 85 1208.0 86 803.5 87 399.0 88 337.5 89 276.0 90 276.0 91 203.5 92 131.0 93 111.5 94 92.0 95 92.0 96 84.5 97 77.0 98 77.0 99 186.5 100 296.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12362353996985453 2 0.05375591202099642 3 0.014154217731018668 4 0.0027103821187057023 5 3.01153568745078E-4 6 3.01153568745078E-4 7 0.0 8 3.01153568745078E-4 9 4.5173035311761703E-4 10 6.02307137490156E-4 11 0.001957498196843007 12 0.0025598053343331632 13 0.013702487377901052 14 0.00903460706235234 15 0.023640555146488627 16 0.009787490984215035 17 0.02017728910592023 18 0.00466788031554871 19 0.006474801728019178 20 0.004818457099921248 21 0.006324224943646638 22 0.007528839218626951 23 0.01054037490607773 24 0.013401333809155973 25 0.015810562359116598 26 0.019273828399684993 27 0.009486337415469958 28 0.0051196106686663264 29 0.011594412396685504 30 0.003463266040568397 31 0.006625378512391716 32 0.005872494590529021 33 0.0072276856498818725 34 0.012799026671665817 35 0.015810562359116598 36 0.012949603456038355 37 0.008733453493607263 38 0.0061736481592740994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 664113.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.49857616578004 #Duplication Level Percentage of deduplicated Percentage of total 1 82.87396981100487 53.452530540156516 2 11.573250573893427 14.929163672518452 3 2.801445269871879 5.420676933392868 4 1.0571278321655635 2.727329599995861 5 0.48222359381059854 1.555136759716453 6 0.2514185552810687 0.972968330237183 7 0.16626042148027054 0.7506492320739943 8 0.10769997361393811 0.555719596095287 9 0.07545379085012413 0.43799958685292095 >10 0.47455524598644355 6.06418464523649 >50 0.07297546644367131 3.3495294481673534 >100 0.059175458810186474 7.040891501025065 >500 0.0030406362233499355 1.1267659114714401 >1k 0.001403370564623047 1.6164542430601798 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 3427 0.5160266400446912 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 2309 0.34768179511619257 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 1490 0.22435940871508311 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 1368 0.20598904102163335 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 1117 0.16819426814412608 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 1004 0.15117909151002917 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA 849 0.12783968993228562 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.50576784372539E-4 2 0.0 0.0 0.0 0.0 1.50576784372539E-4 3 0.0 0.0 0.0 0.0 1.50576784372539E-4 4 0.0 0.0 0.0 0.0 1.50576784372539E-4 5 0.0 0.0 0.0 1.50576784372539E-4 1.50576784372539E-4 6 0.0 0.0 0.0 1.50576784372539E-4 6.02307137490156E-4 7 0.0 0.0 0.0 1.50576784372539E-4 6.02307137490156E-4 8 0.0 0.0 0.0 1.50576784372539E-4 6.02307137490156E-4 9 0.0 0.0 0.0 1.50576784372539E-4 6.02307137490156E-4 10 0.0 0.0 0.0 1.50576784372539E-4 6.02307137490156E-4 11 0.0 0.0 0.0 3.01153568745078E-4 6.02307137490156E-4 12 0.0 0.0 0.0 3.01153568745078E-4 9.034607062352341E-4 13 0.0 0.0 0.0 3.01153568745078E-4 9.034607062352341E-4 14 0.0 0.0 0.0 3.01153568745078E-4 9.034607062352341E-4 15 0.0 0.0 0.0 3.01153568745078E-4 0.001054037490607773 16 0.0 0.0 0.0 3.01153568745078E-4 0.001054037490607773 17 0.0 0.0 0.0 3.01153568745078E-4 0.001054037490607773 18 0.0 0.0 0.0 4.5173035311761703E-4 0.001204614274980312 19 0.0 0.0 0.0 6.02307137490156E-4 0.001204614274980312 20 0.0 0.0 0.0 6.02307137490156E-4 0.001204614274980312 21 0.0 0.0 0.0 9.034607062352341E-4 0.001204614274980312 22 0.0 0.0 0.0 0.0015057678437253902 0.001204614274980312 23 0.0 0.0 0.0 0.003914996393686014 0.0013551910593528511 24 0.0 0.0 0.0 0.006775955296764256 0.0013551910593528511 25 0.0 0.0 0.0 0.008131146356117107 0.0013551910593528511 26 0.0 0.0 0.0 0.01054037490607773 0.0013551910593528511 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAGGT 20 0.0037292202 32.04049 1 TCGACGC 45 0.008847287 17.777485 14 CGCAAGA 165 0.0 17.456886 2 CTATTCC 205 0.0 17.167864 4 GTATTAG 140 4.0017767E-11 17.164547 1 TATGACA 85 4.985466E-6 16.938347 4 GTCCTAT 215 0.0 16.392807 1 CCTATTC 205 0.0 16.38874 3 GTTCTAG 60 0.0024199358 16.020245 1 ATATTAC 60 0.0024199358 16.020245 1 CGCGTAA 90 8.5712145E-6 15.999737 10 TCGCGTA 90 8.5712145E-6 15.999737 9 GCGTAAC 100 1.3125518E-6 15.999736 11 AGACGGA 180 0.0 15.997329 6 CAAGACG 180 0.0 15.997329 4 ACGACGG 110 2.019242E-7 15.997328 6 CAGAGCG 185 0.0 15.56731 14 ACGGACC 175 3.6379788E-12 15.54143 8 TCCAATA 155 1.8735591E-10 15.485949 2 TACGACG 125 5.1355528E-8 15.357434 5 >>END_MODULE