FastQCFastQC Report
Thu 2 Feb 2017
SRR4062812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664113
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40050.6030600214120188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28680.43185421758044185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18330.276007245754864No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14770.22240191051824013No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14070.2118615356121624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.1859623287000857No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8080.12166604177301153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGA453.5350482E-421.3329322
TCTATAC707.775561E-720.5710413
GCTTATA400.00448005620.0011311
ATCGACA400.00448200919.99962415
AGCGTAT751.505794E-619.2010848
CTCTATA701.7857008E-518.285372
CAAGACG1500.018.1329924
GTGTTAG450.00884357517.7787821
TCGACAA450.00884741217.77744316
TAGGTGA550.001363307417.4555326
TCGTTAA1101.0610165E-817.45421812
ATCATAC550.001363995517.4542183
ACCGTCG1302.582965E-1017.231748
TTATATC652.0990244E-417.2304443
TAGCGTA753.228806E-517.067637
ATAGCGT753.228806E-517.067636
GTTTTCG1505.456968E-1217.06634528
GTATATG957.675844E-716.8430561
GACGGAC1456.91216E-1116.552667
TTTTCGG1551.0913936E-1116.51581829
Produced by FastQC (version 0.11.5)