FastQCFastQC Report
Thu 2 Feb 2017
SRR4062811_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062811_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971041
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42050.21333904266831585No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC34070.17285282244255698No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT34010.17254841477168664No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA26920.13657757499717155No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT26640.13515700586644316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26430.13409157901839688No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25990.13185925609868085No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG25810.13094603308606975No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG25090.12729314103562533No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24940.12653212185844942No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG24700.12531449117496796No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22710.11521830342443408No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC21430.10872427311253291No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20970.10639048096919344No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA19890.10091114289352682No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG19880.1008604082817151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5850.020.78550129
TAGCGGC6300.019.5547830
AAGACGG7450.019.3246615
CAAGACG7550.019.2810694
TCTAGCG6400.018.997828
ACGGACC8000.018.99788
GACGGAC7900.018.2257397
GCGCAAG8150.018.0849531
CGCAAGA8000.017.807882
CGGACCA8100.017.7779739
GTGTTAC1453.6379788E-1217.6782461
AGACGGA8600.017.670586
ACGATTA652.1019997E-417.23008732
AGAGCGA8650.016.64800615
GAGCGAA8700.016.55148916
AGCGGCG7550.016.52914631
GCAAGAC9850.016.4050243
AATAGCG4400.016.3600835
CAGAGCG9100.016.35224514
CATCGTT5400.016.29440928
Produced by FastQC (version 0.11.5)