##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062811_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1971041
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27791507127452	32.0	32.0	32.0	32.0	32.0
2	30.908663493047584	32.0	32.0	32.0	32.0	32.0
3	30.9588938028179	32.0	32.0	32.0	32.0	32.0
4	31.02088185887559	32.0	32.0	32.0	32.0	32.0
5	30.926968033643135	32.0	32.0	32.0	32.0	32.0
6	34.592846622672994	36.0	36.0	36.0	32.0	36.0
7	34.533365363784924	36.0	36.0	36.0	32.0	36.0
8	34.52036918562323	36.0	36.0	36.0	32.0	36.0
9	34.662775660171455	36.0	36.0	36.0	32.0	36.0
10	34.38515484964544	36.0	36.0	36.0	32.0	36.0
11	34.63550479163041	36.0	36.0	36.0	32.0	36.0
12	34.459307543577225	36.0	36.0	36.0	32.0	36.0
13	34.54727983842041	36.0	36.0	36.0	32.0	36.0
14	34.44191774803264	36.0	36.0	36.0	32.0	36.0
15	34.390771171173	36.0	36.0	36.0	32.0	36.0
16	34.40176942032154	36.0	36.0	36.0	32.0	36.0
17	34.323268262811375	36.0	36.0	36.0	32.0	36.0
18	34.35899202502637	36.0	36.0	36.0	32.0	36.0
19	34.34933875043695	36.0	36.0	36.0	32.0	36.0
20	34.33296618385919	36.0	36.0	36.0	32.0	36.0
21	34.32329565950175	36.0	36.0	36.0	32.0	36.0
22	34.29784565617864	36.0	36.0	36.0	32.0	36.0
23	34.240309054961315	36.0	36.0	36.0	32.0	36.0
24	34.21682603253814	36.0	36.0	36.0	32.0	36.0
25	34.19977717358492	36.0	36.0	36.0	32.0	36.0
26	34.14631151761937	36.0	36.0	36.0	32.0	36.0
27	34.15019170073073	36.0	36.0	36.0	32.0	36.0
28	34.132577150855816	36.0	36.0	36.0	32.0	36.0
29	34.10881153664485	36.0	36.0	36.0	32.0	36.0
30	34.07830735129305	36.0	36.0	36.0	32.0	36.0
31	34.09582246132881	36.0	36.0	36.0	32.0	36.0
32	34.05464219161347	36.0	36.0	36.0	32.0	36.0
33	34.02541195236426	36.0	36.0	36.0	32.0	36.0
34	34.02975432778923	36.0	36.0	36.0	32.0	36.0
35	33.98998752435895	36.0	36.0	36.0	32.0	36.0
36	33.95598112875379	36.0	36.0	36.0	32.0	36.0
37	33.95178689839531	36.0	36.0	36.0	32.0	36.0
38	33.357025551472546	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	2.0
8	2.0
9	4.0
10	15.0
11	27.0
12	9.0
13	7.0
14	154.0
15	395.0
16	544.0
17	682.0
18	843.0
19	1124.0
20	1566.0
21	2263.0
22	3592.0
23	5382.0
24	8115.0
25	12206.0
26	18053.0
27	25955.0
28	36786.0
29	49189.0
30	66316.0
31	90062.0
32	124365.0
33	187101.0
34	423431.0
35	912849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.01766630094681	16.957413954244384	11.180665610142631	25.84425413466618
2	17.145717225566035	18.939853490377786	37.18771780120844	26.726711482847737
3	19.312416436854804	22.86658057707091	28.088278199628075	29.73272478644622
4	12.585876676500954	14.609270951981108	35.71292671975322	37.09192565176472
5	14.866902481740569	36.16833989840876	33.47325974766569	15.491497872184985
6	35.43450103017152	35.07408051928153	16.16407859642999	13.327339854116957
7	31.25087834385824	29.902046585582525	20.17489253129948	18.67218253925975
8	28.87009674100321	32.64252910652063	18.796764747067023	19.690609405409134
9	27.26559673917841	14.022161938106692	18.455970131458717	40.256271191256175
10	16.048741954092094	26.12660467886636	31.081214774181866	26.743438592859683
11	38.49166364790366	20.97593817573361	21.46203512318075	19.07036305318198
12	25.011111201308584	23.426674189135984	28.13168447841281	23.430530131142618
13	29.544270179236243	18.833581715802758	25.241189779936686	26.380958325024316
14	23.603619328182628	19.609074806315814	24.571012799976458	32.2162930655251
15	25.3222151064307	27.12204236523497	21.929657186876135	25.626085341458193
16	26.292438359695335	25.651895725098424	23.24691792134173	24.80874799386451
17	24.33298387023112	25.927540365018096	24.761169202726826	24.97830656202395
18	25.489094780807303	24.42021231525919	25.79732381095895	24.293369092974558
19	25.70071526461036	24.818215628661118	25.119603993953966	24.361465112774557
20	26.009246415136843	24.035453385870397	24.638012791956672	25.317287407036083
21	27.211980222384017	23.938272325290537	24.2085528802612	24.641194572064247
22	25.995393989455597	23.955669051191254	24.9642930169572	25.084643942395946
23	24.43392789550968	23.786089538486205	25.553336560347706	26.226646005656406
24	24.869200140656467	24.84885265017184	25.071203531228324	25.21074367794337
25	25.08197503250078	24.340114942645386	25.043055286336685	25.534854738517154
26	24.724536987702734	25.393391729360697	25.461134774661765	24.420936508274803
27	25.589978471512936	24.454900026739367	24.713414383546006	25.241707118201695
28	24.574476019611115	24.54504818300867	25.591207774225577	25.289268023154634
29	24.497875000507403	24.8640670095351	25.56012104581637	25.077936944141133
30	24.502645639552732	25.002803175477773	25.786677585098573	24.70787359987093
31	25.323299481259586	24.799941956276434	24.451425927278926	25.42533263518505
32	24.99984778651145	24.630527125458414	24.386681116800514	25.982943971229624
33	24.462079025813406	24.356237711676286	25.23219382254075	25.949489439969557
34	25.477573528665577	24.542012466028954	25.223919675095747	24.756494330209723
35	26.063934781428376	24.3641559688379	25.30725970669707	24.264649543036658
36	24.494075859444376	25.197006215907646	24.799137384244577	25.509780540403398
37	25.823362598187284	24.819430794108737	24.775185947593197	24.582020660110786
38	24.72673970520411	24.541750235803224	25.300278093055894	25.431231965936774
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	100.0
2	150.0
3	150.0
4	409.0
5	668.0
6	668.0
7	836.5
8	1005.0
9	1033.5
10	1062.0
11	1062.0
12	1475.0
13	1888.0
14	2683.0
15	3478.0
16	3478.0
17	5272.0
18	7066.0
19	7066.0
20	8892.5
21	10719.0
22	11818.5
23	12918.0
24	12918.0
25	15741.0
26	18564.0
27	18564.0
28	23638.5
29	28713.0
30	34805.5
31	40898.0
32	40898.0
33	52598.5
34	64299.0
35	64299.0
36	72967.5
37	81636.0
38	93962.5
39	106289.0
40	106289.0
41	112866.0
42	119443.0
43	135848.0
44	152253.0
45	152253.0
46	156400.5
47	160548.0
48	160548.0
49	171271.5
50	181995.0
51	186223.5
52	190452.0
53	190452.0
54	180708.5
55	170965.0
56	170965.0
57	165838.0
58	160711.0
59	144575.5
60	128440.0
61	128440.0
62	121795.5
63	115151.0
64	96232.5
65	77314.0
66	77314.0
67	64660.5
68	52007.0
69	52007.0
70	41677.0
71	31347.0
72	24626.0
73	17905.0
74	17905.0
75	13552.0
76	9199.0
77	9199.0
78	8932.0
79	8665.0
80	6814.5
81	4964.0
82	4964.0
83	4981.0
84	4998.0
85	4998.0
86	3388.5
87	1779.0
88	1493.0
89	1207.0
90	1207.0
91	876.0
92	545.0
93	471.5
94	398.0
95	398.0
96	355.5
97	313.0
98	313.0
99	676.0
100	1039.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1177550340150205
2	0.05418456541492541
3	0.01146602226945051
4	0.002333792143339484
5	2.536730590586396E-4
6	5.073461181172792E-4
7	1.0146922362345583E-4
8	2.536730590586396E-4
9	2.0293844724691165E-4
10	4.058768944938233E-4
11	0.0017757114134104772
12	0.002891872873268491
13	0.014713037425401096
14	0.009030760902487568
15	0.023490125268830025
16	0.010755737704086318
17	0.020243110112879437
18	0.005276399628419703
19	0.008219007113499922
20	0.0058344803583487105
21	0.0063925610882777165
22	0.007001376430018452
23	0.009081495514299296
24	0.01430716053090727
25	0.016184341167941204
26	0.018213725640410322
27	0.008320476337123378
28	0.005986684193783893
29	0.011009410763144958
30	0.003145545932327131
31	0.005733011134725255
32	0.00634182647646599
33	0.008421945560746835
34	0.012429979893873339
35	0.015930668108882565
36	0.014763772037212824
37	0.009639576244228305
38	0.005986684193783893
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1971041.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.95391551623207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.79120483738231	40.93511599543497
2	12.83227981986394	13.333743632837217
3	3.7801660297034108	5.891832796336244
4	1.5411153796952735	3.2026791294981662
5	0.7912431901603327	2.055409092719194
6	0.45920459243002304	1.4314485959865122
7	0.32136154321461113	1.168719332643651
8	0.22644911285280453	0.9411934462304248
9	0.17044894517128517	0.7969941087533841
>10	0.9195582945412992	8.634462695474259
>50	0.07232514037534875	2.64847295698054
>100	0.07479199925858028	8.427463736623139
>500	0.01287374312352486	4.620162117908448
>1k	0.006977372227253932	5.9123023625739615
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4205	0.21333904266831585	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3407	0.17285282244255698	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3401	0.17254841477168664	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2692	0.13657757499717155	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2664	0.13515700586644316	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2643	0.13409157901839688	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2599	0.13185925609868085	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2581	0.13094603308606975	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2509	0.12729314103562533	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2494	0.12653212185844942	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2470	0.12531449117496796	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2271	0.11521830342443408	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2143	0.10872427311253291	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2097	0.10639048096919344	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1989	0.10091114289352682	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1988	0.1008604082817151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.073461181172791E-5	5.073461181172791E-5
2	0.0	0.0	0.0	5.073461181172791E-5	5.073461181172791E-5
3	0.0	0.0	0.0	5.073461181172791E-5	5.073461181172791E-5
4	0.0	0.0	0.0	5.073461181172791E-5	5.073461181172791E-5
5	0.0	0.0	0.0	5.073461181172791E-5	5.073461181172791E-5
6	0.0	0.0	0.0	5.073461181172791E-5	5.073461181172791E-5
7	0.0	0.0	0.0	1.0146922362345583E-4	5.073461181172791E-5
8	0.0	0.0	0.0	1.0146922362345583E-4	5.073461181172791E-5
9	0.0	0.0	0.0	1.0146922362345583E-4	5.073461181172791E-5
10	0.0	0.0	0.0	1.0146922362345583E-4	5.073461181172791E-5
11	0.0	0.0	0.0	1.0146922362345583E-4	5.073461181172791E-5
12	0.0	0.0	0.0	1.0146922362345583E-4	3.044076708703675E-4
13	0.0	0.0	0.0	1.5220383543518374E-4	3.044076708703675E-4
14	0.0	0.0	0.0	1.5220383543518374E-4	3.551422826820954E-4
15	0.0	0.0	0.0	1.5220383543518374E-4	4.058768944938233E-4
16	0.0	0.0	0.0	2.0293844724691165E-4	4.058768944938233E-4
17	0.0	0.0	0.0	2.536730590586396E-4	4.058768944938233E-4
18	0.0	5.073461181172791E-5	0.0	3.044076708703675E-4	4.058768944938233E-4
19	0.0	5.073461181172791E-5	0.0	3.551422826820954E-4	4.058768944938233E-4
20	0.0	5.073461181172791E-5	0.0	4.5661150630555125E-4	4.5661150630555125E-4
21	0.0	5.073461181172791E-5	0.0	6.08815341740735E-4	4.5661150630555125E-4
22	0.0	5.073461181172791E-5	0.0	8.117537889876466E-4	4.5661150630555125E-4
23	0.0	5.073461181172791E-5	0.0	0.00121763068348147	4.5661150630555125E-4
24	0.0	5.073461181172791E-5	0.0	0.001826446025222205	4.5661150630555125E-4
25	0.0	5.073461181172791E-5	0.0	0.002384526755151212	4.5661150630555125E-4
26	0.0	5.073461181172791E-5	0.0	0.002891872873268491	4.5661150630555125E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	585	0.0	20.785501	29
TAGCGGC	630	0.0	19.55478	30
AAGACGG	745	0.0	19.324661	5
CAAGACG	755	0.0	19.281069	4
TCTAGCG	640	0.0	18.9978	28
ACGGACC	800	0.0	18.9978	8
GACGGAC	790	0.0	18.225739	7
GCGCAAG	815	0.0	18.084953	1
CGCAAGA	800	0.0	17.80788	2
CGGACCA	810	0.0	17.777973	9
GTGTTAC	145	3.6379788E-12	17.678246	1
AGACGGA	860	0.0	17.67058	6
ACGATTA	65	2.1019997E-4	17.230087	32
AGAGCGA	865	0.0	16.648006	15
GAGCGAA	870	0.0	16.551489	16
AGCGGCG	755	0.0	16.529146	31
GCAAGAC	985	0.0	16.405024	3
AATAGCG	440	0.0	16.360083	5
CAGAGCG	910	0.0	16.352245	14
CATCGTT	540	0.0	16.294409	28
>>END_MODULE