FastQCFastQC Report
Thu 2 Feb 2017
SRR4062811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971041
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41030.20816411226351964No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC36030.18279680635765566No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32580.16529336528260954No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27690.1404841401066746No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC27620.14012899782399252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27560.13982459015312215No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA27330.1386576940814524No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT26060.13221439838136295No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG26060.13221439838136295No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25340.12856150633091853No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23990.12171233373633526No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC22730.11531977264805755No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21780.1104999845259434No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21590.10953602690152056No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21530.1092316192306502No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20110.10202730435338483No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG20080.10187510051794967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5800.021.24087529
TCTAGCG6200.020.12855328
TAGCGGC6450.019.1003230
ACGTCGT450.00885366717.77735523
AAGACGG8200.017.5618935
CAAGACG7950.017.107384
AGCGGCG7250.016.77201531
CGCAAGA8200.016.584972
ATCGTTT4850.016.49445229
CGCCGGT7350.016.32787
GCGCAAG8400.016.1909121
CGGTCCA7900.016.00083710
ATAACGA3300.016.00002712
TAAACGC4500.015.99961928
GACGGAC8150.015.9030767
ACGGACC8400.015.8107528
TAACGAA3300.015.51478213
CGCTTCG4800.015.33296932
CGGACCA8600.015.2579
GTATCAA33200.015.2293271
Produced by FastQC (version 0.11.5)