##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062811_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1971041
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.200918702350688	32.0	32.0	32.0	32.0	32.0
2	31.268527646050995	32.0	32.0	32.0	32.0	32.0
3	31.368583910735495	32.0	32.0	32.0	32.0	32.0
4	31.470016605438445	32.0	32.0	32.0	32.0	32.0
5	31.38710407343125	32.0	32.0	32.0	32.0	32.0
6	34.924364333364956	36.0	36.0	36.0	36.0	36.0
7	34.95099797518164	36.0	36.0	36.0	36.0	36.0
8	34.880322631543436	36.0	36.0	36.0	32.0	36.0
9	35.00002130853696	36.0	36.0	36.0	36.0	36.0
10	34.84229501060607	36.0	36.0	36.0	32.0	36.0
11	35.02383004716797	36.0	36.0	36.0	36.0	36.0
12	34.9125538230813	36.0	36.0	36.0	32.0	36.0
13	34.973498268174026	36.0	36.0	36.0	36.0	36.0
14	34.92320606217729	36.0	36.0	36.0	32.0	36.0
15	34.887716693868875	36.0	36.0	36.0	32.0	36.0
16	34.89586822394867	36.0	36.0	36.0	32.0	36.0
17	34.86044125921277	36.0	36.0	36.0	32.0	36.0
18	34.87262213216265	36.0	36.0	36.0	32.0	36.0
19	34.859578263465856	36.0	36.0	36.0	32.0	36.0
20	34.84673378179348	36.0	36.0	36.0	32.0	36.0
21	34.84236553171649	36.0	36.0	36.0	32.0	36.0
22	34.82535066495319	36.0	36.0	36.0	32.0	36.0
23	34.77368050689965	36.0	36.0	36.0	32.0	36.0
24	34.742731379002265	36.0	36.0	36.0	32.0	36.0
25	34.72101138433955	36.0	36.0	36.0	32.0	36.0
26	34.66140125953747	36.0	36.0	36.0	32.0	36.0
27	34.64552234073264	36.0	36.0	36.0	32.0	36.0
28	34.61764012011927	36.0	36.0	36.0	32.0	36.0
29	34.57952371361123	36.0	36.0	36.0	32.0	36.0
30	34.560373934382895	36.0	36.0	36.0	32.0	36.0
31	34.55940591798953	36.0	36.0	36.0	32.0	36.0
32	34.53261398418399	36.0	36.0	36.0	32.0	36.0
33	34.49727529767265	36.0	36.0	36.0	32.0	36.0
34	34.4949019325321	36.0	36.0	36.0	32.0	36.0
35	34.46354692774021	36.0	36.0	36.0	32.0	36.0
36	34.435949835645225	36.0	36.0	36.0	32.0	36.0
37	34.44086348279919	36.0	36.0	36.0	32.0	36.0
38	33.9995352709558	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	37.0
22	157.0
23	489.0
24	1352.0
25	3203.0
26	6532.0
27	12915.0
28	22534.0
29	35265.0
30	53199.0
31	77759.0
32	113504.0
33	183593.0
34	453142.0
35	1007352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35091093872328	18.14758795441019	11.907529584515336	26.593971522351197
2	16.171200903481967	19.914146889892194	36.43222033433094	27.482431872294892
3	18.294698080861842	23.502200106441215	28.415999464242496	29.787102348454447
4	12.106380657877713	15.452338492213483	35.67100533476067	36.77027551514813
5	14.351720082656913	36.69546873501742	33.13952691955867	15.813284262766997
6	35.46506967394845	35.40591302653729	15.833890569334534	13.295126730179732
7	30.969645312349382	30.129424717026122	20.14058639391587	18.760343576708625
8	28.73360801498116	32.848150363595494	18.626551172521282	19.79169044890206
9	27.10805046048439	13.970444979319808	18.213030348539398	40.7084742116564
10	15.831549147142088	26.40130490811864	31.043672819149474	26.7234731255898
11	38.60072290672636	20.879005465434627	21.402920039859318	19.117351587979698
12	24.80837488508581	23.505151605551596	28.091572147845095	23.594901361517493
13	29.484297368037872	18.795011159089192	25.22583266997761	26.494858802895326
14	23.444311914364032	19.468392590514352	24.52526355362471	32.562031941496905
15	25.329102743169724	27.202934895824082	21.727655589102408	25.740306771903782
16	26.30726605889984	25.727217242056355	23.105607645908936	24.859909053134864
17	24.34677918927105	25.996719500000253	24.604916894169122	25.051584416559574
18	25.523923652526758	24.4577357853033	25.700277163184325	24.31806339898561
19	25.692819175248005	24.829569755271454	25.006481346658948	24.471129722821594
20	25.9899717966293	23.956021209097123	24.660268355655717	25.39373863861787
21	27.17465255634211	24.03113281878238	24.087448295036275	24.706766329839237
22	25.936662878480398	24.087080931893823	24.86474145628704	25.111514733338748
23	24.29846608741181	23.727853039870364	25.56708698665373	26.406593886064094
24	24.846210707945698	24.887153539677765	25.00176302776046	25.264872724616076
25	25.048692543686307	24.34206087037256	25.107950570282405	25.50129601565873
26	24.80912370671133	25.29845903763544	25.325602054954715	24.566815200698514
27	25.719886821113132	24.358878743647384	24.637513514445935	25.283720920793552
28	24.6156218972614	24.468491523007387	25.62904576820066	25.28684081153056
29	24.498729351647174	24.783147585463723	25.487597670469565	25.23052539241954
30	24.466360669311292	24.901460700208673	25.788250980065865	24.843927650414173
31	25.2857246500707	24.730586527626773	24.481479583631188	25.50220923867134
32	25.082989141271035	24.533888437632704	24.51364532751982	25.86947709357644
33	24.479906810665025	24.129584316105042	25.32210136673971	26.068407506490228
34	25.466999418074003	24.426838406709958	25.290392234357377	24.81576994085866
35	26.012700902720947	24.300001877180637	25.274410831636686	24.412886388461732
36	24.602025021295855	25.143769206221485	24.817647121495696	25.436558650986967
37	25.89925831070992	24.71769993622659	24.65098392169417	24.73205783136931
38	24.782335831674736	24.329022075136944	25.317890393959335	25.57075169922899
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	44.0
2	60.0
3	60.0
4	275.5
5	491.0
6	491.0
7	686.0
8	881.0
9	977.0
10	1073.0
11	1073.0
12	1459.0
13	1845.0
14	2660.0
15	3475.0
16	3475.0
17	5204.5
18	6934.0
19	6934.0
20	8646.5
21	10359.0
22	11837.5
23	13316.0
24	13316.0
25	16014.0
26	18712.0
27	18712.0
28	23709.0
29	28706.0
30	34757.5
31	40809.0
32	40809.0
33	52644.5
34	64480.0
35	64480.0
36	72933.5
37	81387.0
38	93360.0
39	105333.0
40	105333.0
41	112683.0
42	120033.0
43	136376.0
44	152719.0
45	152719.0
46	155971.0
47	159223.0
48	159223.0
49	171156.0
50	183089.0
51	186511.0
52	189933.0
53	189933.0
54	181476.0
55	173019.0
56	173019.0
57	167331.5
58	161644.0
59	145490.0
60	129336.0
61	129336.0
62	122803.0
63	116270.0
64	96579.5
65	76889.0
66	76889.0
67	64611.5
68	52334.0
69	52334.0
70	42001.0
71	31668.0
72	24749.5
73	17831.0
74	17831.0
75	13440.5
76	9050.0
77	9050.0
78	8880.5
79	8711.0
80	6776.5
81	4842.0
82	4842.0
83	4541.5
84	4241.0
85	4241.0
86	2756.5
87	1272.0
88	1031.0
89	790.0
90	790.0
91	473.0
92	156.0
93	105.0
94	54.0
95	54.0
96	43.0
97	32.0
98	32.0
99	24.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008219007113499922
2	0.0
3	0.0
4	3.044076708703675E-4
5	1.0146922362345583E-4
6	1.5220383543518374E-4
7	5.580807299290071E-4
8	0.0016235075779752932
9	0.0037036266622561377
10	0.0010654268480462864
11	0.005530072687478343
12	2.536730590586396E-4
13	1.0146922362345583E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0146922362345583E-4
22	5.073461181172791E-5
23	1.5220383543518374E-4
24	0.0
25	0.0
26	0.0
27	1.0146922362345583E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1971041.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.89840608154098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.05480006640842	38.449117042455974
2	13.874215265952166	13.846024548063927
3	4.069778222990762	6.092263392978159
4	1.655694965431633	3.3046615892908235
5	0.8981209234018966	2.24074012731182
6	0.523386902215983	1.5669703310719745
7	0.3312047947006166	1.1568613939487347
8	0.24507836555981263	0.9783215845203104
9	0.18220352799547673	0.8182499066466947
>10	0.9850590871154292	8.88255862865696
>50	0.07805838230430648	2.7154249266382506
>100	0.08054591387984368	8.694502636561781
>500	0.014056059812078664	4.819674801510537
>1k	0.0077975222315180915	6.434629090344012
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4103	0.20816411226351964	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3603	0.18279680635765566	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3258	0.16529336528260954	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2769	0.1404841401066746	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2762	0.14012899782399252	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2756	0.13982459015312215	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2733	0.1386576940814524	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2606	0.13221439838136295	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2606	0.13221439838136295	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2534	0.12856150633091853	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2399	0.12171233373633526	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2273	0.11531977264805755	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2178	0.1104999845259434	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2159	0.10953602690152056	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2153	0.1092316192306502	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2011	0.10202730435338483	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2008	0.10187510051794967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.536730590586396E-4
2	0.0	0.0	0.0	0.0	2.536730590586396E-4
3	0.0	0.0	0.0	0.0	2.536730590586396E-4
4	0.0	0.0	0.0	0.0	2.536730590586396E-4
5	0.0	0.0	0.0	0.0	3.551422826820954E-4
6	0.0	0.0	0.0	0.0	3.551422826820954E-4
7	0.0	0.0	0.0	5.073461181172791E-5	3.551422826820954E-4
8	0.0	0.0	0.0	5.073461181172791E-5	3.551422826820954E-4
9	0.0	0.0	0.0	1.5220383543518374E-4	3.551422826820954E-4
10	0.0	0.0	0.0	1.5220383543518374E-4	3.551422826820954E-4
11	0.0	0.0	0.0	1.5220383543518374E-4	3.551422826820954E-4
12	0.0	0.0	0.0	1.5220383543518374E-4	7.610191771759187E-4
13	0.0	0.0	0.0	2.0293844724691165E-4	7.610191771759187E-4
14	0.0	0.0	0.0	2.0293844724691165E-4	8.117537889876466E-4
15	0.0	0.0	0.0	2.0293844724691165E-4	0.001166896071669742
16	0.0	0.0	0.0	2.0293844724691165E-4	0.001268365295293198
17	0.0	0.0	0.0	2.536730590586396E-4	0.001268365295293198
18	0.0	0.0	0.0	3.044076708703675E-4	0.001268365295293198
19	0.0	0.0	0.0	3.551422826820954E-4	0.001268365295293198
20	0.0	0.0	0.0	4.5661150630555125E-4	0.0013190999071049257
21	0.0	0.0	0.0	6.595499535524628E-4	0.0013190999071049257
22	0.0	0.0	0.0	8.117537889876466E-4	0.0013190999071049257
23	0.0	0.0	0.0	0.001166896071669742	0.0013190999071049257
24	0.0	0.0	0.0	0.0016742421897870212	0.0013190999071049257
25	0.0	0.0	0.0	0.0020801190842808445	0.0013190999071049257
26	0.0	0.0	0.0	0.0024859959787746676	0.0014205691307283817
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	580	0.0	21.240875	29
TCTAGCG	620	0.0	20.128553	28
TAGCGGC	645	0.0	19.10032	30
ACGTCGT	45	0.008853667	17.777355	23
AAGACGG	820	0.0	17.561893	5
CAAGACG	795	0.0	17.10738	4
AGCGGCG	725	0.0	16.772015	31
CGCAAGA	820	0.0	16.58497	2
ATCGTTT	485	0.0	16.494452	29
CGCCGGT	735	0.0	16.3278	7
GCGCAAG	840	0.0	16.190912	1
CGGTCCA	790	0.0	16.000837	10
ATAACGA	330	0.0	16.000027	12
TAAACGC	450	0.0	15.999619	28
GACGGAC	815	0.0	15.903076	7
ACGGACC	840	0.0	15.810752	8
TAACGAA	330	0.0	15.514782	13
CGCTTCG	480	0.0	15.332969	32
CGGACCA	860	0.0	15.257	9
GTATCAA	3320	0.0	15.229327	1
>>END_MODULE