FastQCFastQC Report
Thu 2 Feb 2017
SRR4062810_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062810_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456956
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35370.24276642534160264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23130.15875565219539917No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21750.14928384934068015No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21350.14653839923786305No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21320.1463324904801518No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20440.14029250025395412No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20090.13789023141398915No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18900.12972251735810827No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18880.12958524485296744No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18560.12738888477071372No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17640.1210743495342344No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17440.11970162448282583No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16910.11606390309659316No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16330.11208300044750837No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16230.1113966379218041No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15880.10899436908183913No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15740.10803346154585314No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15550.10672937274701501No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15310.10508210268532475No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15070.10343483262363448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4600.020.17248528
CTAGCGG5000.018.55868529
ACCGCAT450.00885674117.775918
GCGTGTA550.001365216817.4539099
TAGCGGC5350.017.34456630
CGAACGA2000.016.79996316
GTCCTAT5750.016.7151241
CCTATTC5700.015.7192653
ACGAACG2150.015.62787315
CCGGTCC6700.015.5218239
TAACGAA2400.015.33330113
CGGTCCA6450.015.13175110
AGCGGCG6350.015.11704131
CGCCGGT6100.014.9492487
CTATTCC6000.014.9302254
GCCGGTC6550.014.8991988
GATATAC2050.014.8465621
TAGCGTA3250.014.7676797
ATAACGA2500.014.71996912
CATCGTT3950.014.58124628
Produced by FastQC (version 0.11.5)