##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062810_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1456956
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249709668651626	32.0	32.0	32.0	32.0	32.0
2	30.92734921301673	32.0	32.0	32.0	32.0	32.0
3	30.961821770870227	32.0	32.0	32.0	32.0	32.0
4	31.023403589401465	32.0	32.0	32.0	32.0	32.0
5	30.972442544592973	32.0	32.0	32.0	32.0	32.0
6	34.602824656338285	36.0	36.0	36.0	32.0	36.0
7	34.532359247636855	36.0	36.0	36.0	32.0	36.0
8	34.526065989638674	36.0	36.0	36.0	32.0	36.0
9	34.63213164982333	36.0	36.0	36.0	32.0	36.0
10	34.398091637633534	36.0	36.0	36.0	32.0	36.0
11	34.61605841219639	36.0	36.0	36.0	32.0	36.0
12	34.44976100856855	36.0	36.0	36.0	32.0	36.0
13	34.53274910155145	36.0	36.0	36.0	32.0	36.0
14	34.44068249144106	36.0	36.0	36.0	32.0	36.0
15	34.39624189062676	36.0	36.0	36.0	32.0	36.0
16	34.40167719546781	36.0	36.0	36.0	32.0	36.0
17	34.33150623628991	36.0	36.0	36.0	32.0	36.0
18	34.35792638899184	36.0	36.0	36.0	32.0	36.0
19	34.34667690719555	36.0	36.0	36.0	32.0	36.0
20	34.33007860223644	36.0	36.0	36.0	32.0	36.0
21	34.308510346228715	36.0	36.0	36.0	32.0	36.0
22	34.28793525679568	36.0	36.0	36.0	32.0	36.0
23	34.2322108560588	36.0	36.0	36.0	32.0	36.0
24	34.21778077031839	36.0	36.0	36.0	32.0	36.0
25	34.181616328839034	36.0	36.0	36.0	32.0	36.0
26	34.139739978420764	36.0	36.0	36.0	32.0	36.0
27	34.14753225217508	36.0	36.0	36.0	32.0	36.0
28	34.128014847394155	36.0	36.0	36.0	32.0	36.0
29	34.09355327134107	36.0	36.0	36.0	32.0	36.0
30	34.06259420325665	36.0	36.0	36.0	32.0	36.0
31	34.08071005576009	36.0	36.0	36.0	32.0	36.0
32	34.04076169767652	36.0	36.0	36.0	32.0	36.0
33	34.00811555050393	36.0	36.0	36.0	32.0	36.0
34	34.01300313804947	36.0	36.0	36.0	32.0	36.0
35	33.97057083398538	36.0	36.0	36.0	32.0	36.0
36	33.93660961621353	36.0	36.0	36.0	32.0	36.0
37	33.92990797251255	36.0	36.0	36.0	32.0	36.0
38	33.341385738484895	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	8.0
9	3.0
10	8.0
11	14.0
12	9.0
13	9.0
14	275.0
15	457.0
16	603.0
17	695.0
18	824.0
19	1094.0
20	1338.0
21	1948.0
22	2879.0
23	4175.0
24	6028.0
25	9123.0
26	13101.0
27	19021.0
28	26995.0
29	36013.0
30	48693.0
31	65898.0
32	89899.0
33	136130.0
34	308844.0
35	682870.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.208365688965365	17.49210751644811	11.478907792977099	25.82061900160942
2	16.762191621273427	19.749973217302447	37.500171683958676	25.98766347746545
3	18.830707937144254	23.85460970513373	28.67642415272969	28.638258204992322
4	12.516550336703688	15.303870702489295	36.24902963033635	35.930549330470676
5	14.711799213838745	36.49657881894612	33.49469749538935	15.29692447182579
6	34.071105931351255	35.66182299634923	16.88311153482645	13.38395953747307
7	30.14238600368577	30.502657940705102	21.064068553936117	18.29088750167301
8	28.034504935306675	32.78888583075203	19.70079982099604	19.475809412945253
9	27.55004969278329	14.176513708069999	18.866990813698735	39.40644578544797
10	16.156763746581888	26.060850657266165	31.335658748723354	26.446726847428593
11	37.687011515317494	21.07714240021305	22.377892127893674	18.857953956575784
12	25.08974448732046	23.741071882074028	28.37456431882733	22.794619311778185
13	29.3955195294641	19.15986715378775	25.42043025367915	26.024183063069
14	23.665107773666033	20.094618146722652	24.7924393346252	31.447834744986107
15	25.15338855344142	27.38331519742962	22.285947706809374	25.17734854231959
16	25.887319592936453	25.60669973056924	23.898886238437644	24.607094438056667
17	24.031491500686165	25.907718204891093	25.262949774379106	24.797840520043636
18	25.176147576147578	24.315538395538397	26.58340626340626	23.924907764907765
19	25.676254489143684	24.808284735264078	25.398188417734723	24.117272357857512
20	25.864164863141436	24.035791824796878	25.03849007700761	25.061553235054078
21	27.23673471909241	24.099523154824702	24.168712723948033	24.495029402134854
22	25.81436310270695	24.34536573579601	24.927926394315385	24.91234476718166
23	23.977575615712997	24.10607582198603	25.742050937426853	26.174297624874125
24	24.886939019836127	25.131595567075983	25.252619554950247	24.728845858137642
25	24.94583603352262	24.412090989971773	25.336379512977388	25.305693463528222
26	24.334115253204054	25.584157301133885	25.933721478631753	24.148005967030304
27	25.370223678664928	24.660665475946956	24.938324654624928	25.030786190763184
28	24.255135135135138	24.679399399399397	25.904624624624624	25.16084084084084
29	24.29322297189229	24.906214970047138	25.69754439386898	25.10301766419159
30	24.199573069029658	25.10971851384094	26.1513065323184	24.539401884811003
31	25.261571218826127	24.81836047029623	24.525952573635653	25.39411573724199
32	24.60068174111684	24.98287410303544	24.60665349677182	25.809790659075905
33	24.133634992044392	24.52056994825766	25.604221208456146	25.7415738512418
34	25.18022847932607	24.688796110826612	25.709209877797473	24.421765532049847
35	25.92103664375874	24.510246097174367	25.595231965928495	23.9734852931384
36	24.280718759524746	25.383255121738244	25.119925971283667	25.21610014745334
37	25.502656834217802	25.098073941058846	24.846771265141825	24.552497959581522
38	24.5391820524331	25.00648652655769	25.306102277079706	25.148229143929502
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	503.0
1	394.5
2	286.0
3	286.0
4	666.0
5	1046.0
6	1046.0
7	1217.5
8	1389.0
9	1267.0
10	1145.0
11	1145.0
12	1517.5
13	1890.0
14	2529.0
15	3168.0
16	3168.0
17	4778.0
18	6388.0
19	6388.0
20	7684.0
21	8980.0
22	9990.5
23	11001.0
24	11001.0
25	13241.0
26	15481.0
27	15481.0
28	19914.5
29	24348.0
30	29796.5
31	35245.0
32	35245.0
33	44808.0
34	54371.0
35	54371.0
36	60054.0
37	65737.0
38	74211.0
39	82685.0
40	82685.0
41	86198.5
42	89712.0
43	100414.0
44	111116.0
45	111116.0
46	113951.0
47	116786.0
48	116786.0
49	124298.5
50	131811.0
51	132677.5
52	133544.0
53	133544.0
54	126773.5
55	120003.0
56	120003.0
57	117022.5
58	114042.0
59	102279.5
60	90517.0
61	90517.0
62	87009.5
63	83502.0
64	68955.0
65	54408.0
66	54408.0
67	45804.0
68	37200.0
69	37200.0
70	29973.5
71	22747.0
72	17830.0
73	12913.0
74	12913.0
75	9837.5
76	6762.0
77	6762.0
78	6676.5
79	6591.0
80	5190.5
81	3790.0
82	3790.0
83	3915.0
84	4040.0
85	4040.0
86	2665.0
87	1290.0
88	1112.5
89	935.0
90	935.0
91	664.5
92	394.0
93	347.5
94	301.0
95	301.0
96	262.5
97	224.0
98	224.0
99	444.5
100	665.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12176071205993867
2	0.05435991203577871
3	0.010981800411268425
4	0.0025395413451058235
5	2.05908757711283E-4
6	2.05908757711283E-4
7	6.863625257042765E-5
8	3.431812628521383E-4
9	2.745450102817106E-4
10	8.23635030845132E-4
11	0.0018531788194015466
12	0.0028827226079579616
13	0.016060883101480072
14	0.010501346643275433
15	0.024777687177924387
16	0.012423161715247407
17	0.021208602044262146
18	0.00555953645820464
19	0.007412715277606187
20	0.005971353973627206
21	0.006108626478768061
22	0.007687260287887897
23	0.010226801632993722
24	0.015099975565494085
25	0.018394515688874614
26	0.019561331982571883
27	0.009197257844437307
28	0.00555953645820464
29	0.01146225417926142
30	0.0031572676182396726
31	0.005971353973627206
32	0.00672635275190191
33	0.008373622813592175
34	0.013315432998662965
35	0.017502244405459052
36	0.015374520575775794
37	0.010089529127852865
38	0.006177262731338489
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1456956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.84457206214144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.21927621867987	43.732171711916784
2	11.841509663656453	12.752020408185881
3	3.2168075589357596	5.1962287925147335
4	1.256344512168218	2.705893304812702
5	0.6622972870575357	1.7830556999765126
6	0.3916388476629773	1.2652575693193926
7	0.25226337254931286	0.9508109339309215
8	0.178267464867799	0.7678988286727577
9	0.13785542956047267	0.6680489950013699
>10	0.6574284268398359	6.527269643074204
>50	0.07648693251730518	2.955502442793265
>100	0.08996112672786336	10.675236515524402
>500	0.014224584672303938	5.352929536322471
>1k	0.005638574104336697	4.667675617954676
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3537	0.24276642534160264	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2313	0.15875565219539917	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2175	0.14928384934068015	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2135	0.14653839923786305	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2132	0.1463324904801518	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2044	0.14029250025395412	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2009	0.13789023141398915	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1890	0.12972251735810827	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1888	0.12958524485296744	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1856	0.12738888477071372	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1764	0.1210743495342344	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1744	0.11970162448282583	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1691	0.11606390309659316	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1633	0.11208300044750837	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1623	0.1113966379218041	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1588	0.10899436908183913	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1574	0.10803346154585314	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1555	0.10672937274701501	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1531	0.10508210268532475	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1507	0.10343483262363448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0590875771128297E-4
2	0.0	0.0	0.0	0.0	2.0590875771128297E-4
3	0.0	0.0	0.0	0.0	2.0590875771128297E-4
4	0.0	0.0	0.0	6.863625257042766E-5	2.0590875771128297E-4
5	0.0	0.0	0.0	6.863625257042766E-5	2.0590875771128297E-4
6	0.0	0.0	0.0	6.863625257042766E-5	2.0590875771128297E-4
7	0.0	0.0	0.0	1.3727250514085533E-4	2.0590875771128297E-4
8	0.0	0.0	0.0	1.3727250514085533E-4	2.0590875771128297E-4
9	0.0	0.0	0.0	1.3727250514085533E-4	2.0590875771128297E-4
10	0.0	0.0	0.0	1.3727250514085533E-4	2.0590875771128297E-4
11	0.0	0.0	0.0	1.3727250514085533E-4	2.0590875771128297E-4
12	0.0	0.0	0.0	1.3727250514085533E-4	5.490900205634213E-4
13	0.0	0.0	0.0	1.3727250514085533E-4	6.17726273133849E-4
14	0.0	0.0	0.0	1.3727250514085533E-4	6.17726273133849E-4
15	0.0	0.0	0.0	1.3727250514085533E-4	7.549987782747042E-4
16	0.0	0.0	0.0	2.0590875771128297E-4	7.549987782747042E-4
17	0.0	0.0	0.0	2.0590875771128297E-4	7.549987782747042E-4
18	0.0	0.0	0.0	2.7454501028171066E-4	7.549987782747042E-4
19	0.0	0.0	0.0	2.7454501028171066E-4	7.549987782747042E-4
20	0.0	0.0	0.0	3.431812628521383E-4	7.549987782747042E-4
21	0.0	0.0	0.0	5.490900205634213E-4	7.549987782747042E-4
22	0.0	0.0	0.0	8.922712834155596E-4	7.549987782747042E-4
23	0.0	0.0	0.0	0.0017159063142606914	7.549987782747042E-4
24	0.0	0.0	0.0	0.0025395413451058235	7.549987782747042E-4
25	0.0	0.0	0.0	0.0030886313656692446	7.549987782747042E-4
26	0.0	0.0	0.0	0.004735901427359509	7.549987782747042E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	460	0.0	20.172485	28
CTAGCGG	500	0.0	18.558685	29
ACCGCAT	45	0.008856741	17.77591	8
GCGTGTA	55	0.0013652168	17.453909	9
TAGCGGC	535	0.0	17.344566	30
CGAACGA	200	0.0	16.799963	16
GTCCTAT	575	0.0	16.715124	1
CCTATTC	570	0.0	15.719265	3
ACGAACG	215	0.0	15.627873	15
CCGGTCC	670	0.0	15.521823	9
TAACGAA	240	0.0	15.333301	13
CGGTCCA	645	0.0	15.131751	10
AGCGGCG	635	0.0	15.117041	31
CGCCGGT	610	0.0	14.949248	7
CTATTCC	600	0.0	14.930225	4
GCCGGTC	655	0.0	14.899198	8
GATATAC	205	0.0	14.846562	1
TAGCGTA	325	0.0	14.767679	7
ATAACGA	250	0.0	14.719969	12
CATCGTT	395	0.0	14.581246	28
>>END_MODULE