FastQCFastQC Report
Thu 2 Feb 2017
SRR4062810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456956
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39730.27269183146230913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27510.1888183308212465No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23140.15882428844796959No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22790.15642201960800464No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22550.15477474954631437No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21750.14928384934068015No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21150.1451656741864545No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19440.13342887499691136No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19410.1332229662392001No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19320.13260523996606624No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17870.12265298334335423No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17800.12217252957536122No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17750.1218293483125091No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17420.11956435197768497No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16670.1144166330349029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16640.11421072427719163No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16140.11077891164867024No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15750.10810209779842357No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15670.10755300777786014No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15590.10700391775729673No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT15220.1044643764121909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG652.0987378E-417.233085
CTTACTC1404.0017767E-1117.143983
CTAGCGG4200.016.76127629
GTAGGAC5600.016.5725143
GTATAGA1456.91216E-1116.5511041
ACGAACG2250.016.35494215
TAGCGGC4350.016.183330
TCTAGCG4350.016.183328
TGTAGGA6300.015.99994852
CGAACGA2300.015.99939916
GCATATA600.002442939415.9993981
TAACGAA2350.015.65898613
ATAACGA2350.015.65898612
AAGACGG5450.015.561725
CCGATAA2600.015.386159
GTAATAA1153.347959E-715.3037731
GATAACG2450.015.0203611
ACGTTTT3200.014.99943629
CGCAAGA5250.014.9332862
ACCGTCG4750.014.8230398
Produced by FastQC (version 0.11.5)