##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062810_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1456956
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199744535867932	32.0	32.0	32.0	32.0	32.0
2	31.287371066799547	32.0	32.0	32.0	32.0	32.0
3	31.36720051943916	32.0	32.0	32.0	32.0	32.0
4	31.467130098644024	32.0	32.0	32.0	32.0	32.0
5	31.390921894690024	32.0	32.0	32.0	32.0	32.0
6	34.904419213758	36.0	36.0	36.0	36.0	36.0
7	34.928441215795125	36.0	36.0	36.0	36.0	36.0
8	34.87038043702075	36.0	36.0	36.0	32.0	36.0
9	34.97171088214057	36.0	36.0	36.0	36.0	36.0
10	34.81887922490453	36.0	36.0	36.0	32.0	36.0
11	34.9985099069567	36.0	36.0	36.0	36.0	36.0
12	34.887376145882236	36.0	36.0	36.0	32.0	36.0
13	34.94594826473826	36.0	36.0	36.0	36.0	36.0
14	34.88785179511255	36.0	36.0	36.0	32.0	36.0
15	34.85200582584512	36.0	36.0	36.0	32.0	36.0
16	34.87092266341605	36.0	36.0	36.0	32.0	36.0
17	34.83210199896222	36.0	36.0	36.0	32.0	36.0
18	34.83565529775779	36.0	36.0	36.0	32.0	36.0
19	34.82834828230914	36.0	36.0	36.0	32.0	36.0
20	34.81701643700976	36.0	36.0	36.0	32.0	36.0
21	34.805421714863044	36.0	36.0	36.0	32.0	36.0
22	34.78316366451698	36.0	36.0	36.0	32.0	36.0
23	34.7258798481217	36.0	36.0	36.0	32.0	36.0
24	34.697152144608346	36.0	36.0	36.0	32.0	36.0
25	34.68039391718075	36.0	36.0	36.0	32.0	36.0
26	34.6064033505473	36.0	36.0	36.0	32.0	36.0
27	34.59006037244776	36.0	36.0	36.0	32.0	36.0
28	34.56172458193659	36.0	36.0	36.0	32.0	36.0
29	34.52142617896491	36.0	36.0	36.0	32.0	36.0
30	34.49857648412169	36.0	36.0	36.0	32.0	36.0
31	34.49391333712205	36.0	36.0	36.0	32.0	36.0
32	34.46527211528694	36.0	36.0	36.0	32.0	36.0
33	34.43086201642328	36.0	36.0	36.0	32.0	36.0
34	34.428015671029186	36.0	36.0	36.0	32.0	36.0
35	34.38433281444326	36.0	36.0	36.0	32.0	36.0
36	34.355973687606216	36.0	36.0	36.0	32.0	36.0
37	34.347689291920965	36.0	36.0	36.0	32.0	36.0
38	33.90989501398807	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	20.0
22	123.0
23	380.0
24	1062.0
25	2473.0
26	5388.0
27	10439.0
28	17983.0
29	28080.0
30	42120.0
31	60182.0
32	85442.0
33	135224.0
34	328380.0
35	739653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.6352199185619	18.72300612570462	12.173979895827328	26.46779405990615
2	15.666361921705255	20.869813501574516	36.78807047021324	26.675754106506993
3	17.875007893169045	24.664986451203745	28.937249992450013	28.5227556631772
4	11.988880881293113	16.24935653248224	36.29994165894506	35.46182092727959
5	14.107014899557708	37.057261852794454	33.19969854957871	15.636024698069125
6	34.16623425827547	36.16807920074457	16.449707472291543	13.215979068688416
7	29.91847329962813	30.752478128908002	20.95355627456337	18.375492296900504
8	27.970609129564867	32.99581650267861	19.50820741086474	19.525366956891787
9	27.47458287917018	13.974131232788704	18.570147958805457	39.98113792923566
10	15.969102598508803	26.40727107500527	31.22768027202013	26.395946054465796
11	37.69595881595881	20.998918918918918	22.372063492063493	18.93305877305877
12	24.954322385805963	23.7923744809362	28.38443323381036	22.868869899447475
13	29.380611879304357	19.121468488366826	25.380416952079475	26.117502680249345
14	23.641482652873524	20.121746984809423	24.692509588484484	31.544260773832566
15	25.145714764207018	27.488681881951138	22.06765338143362	25.29794997240823
16	25.827839569513127	25.6630438139819	23.81549189920073	24.693624717304242
17	24.044377455461934	26.032975601184937	25.0803044155074	24.84234252784573
18	25.195270138562865	24.36641875252238	26.469227622522574	23.969083486392176
19	25.6612416572635	24.919489675734887	25.31908993819992	24.100178728801694
20	25.896320822317215	24.058722432249155	24.96005370100401	25.08490304442962
21	27.204596432562134	24.138477757735995	24.159617723527685	24.49730808617419
22	25.814918226768686	24.44287953788584	24.94811099305676	24.79409124228872
23	23.9695253783589	24.00212773259206	25.776313531692917	26.25203335735612
24	24.824222557167136	25.093276667243213	25.181131070533358	24.901369705056293
25	24.946686754223705	24.46458538527271	25.334070029616562	25.254657830887023
26	24.515393073494426	25.5227687353204	25.874873194349306	24.086964996835864
27	25.46930102117157	24.593089418059872	24.78389846213401	25.153711098634545
28	24.286869335793256	24.665329632466594	25.871199953876438	25.176601077863708
29	24.44637998676693	24.658946460977546	25.725828370932273	25.168845181323253
30	24.32146200708875	24.887779727047352	26.16015857719794	24.630599688665956
31	25.194721048542302	24.72236635835262	24.633894228789337	25.44901836431574
32	24.637875131438424	24.851814330700446	24.710835467920788	25.799475069940343
33	24.214595361836597	24.377469189186222	25.719239290685515	25.68869615829167
34	25.229313719837798	24.51535942060021	25.732280178673893	24.523046680888097
35	25.98493022438564	24.440339996540732	25.504956910160637	24.069772868912995
36	24.333610623793717	25.220459643256216	25.228901902322377	25.217027830627693
37	25.620197178226384	24.93726646515063	24.83499844882069	24.607537907802296
38	24.659787021562092	24.626772961416105	25.475186261758253	25.238253755263546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	284.0
1	234.5
2	185.0
3	185.0
4	556.5
5	928.0
6	928.0
7	1060.0
8	1192.0
9	1203.0
10	1214.0
11	1214.0
12	1576.5
13	1939.0
14	2646.0
15	3353.0
16	3353.0
17	4965.0
18	6577.0
19	6577.0
20	7927.5
21	9278.0
22	10175.5
23	11073.0
24	11073.0
25	13179.5
26	15286.0
27	15286.0
28	19731.5
29	24177.0
30	29693.0
31	35209.0
32	35209.0
33	44757.5
34	54306.0
35	54306.0
36	60449.5
37	66593.0
38	74754.5
39	82916.0
40	82916.0
41	85968.5
42	89021.0
43	100595.5
44	112170.0
45	112170.0
46	114393.0
47	116616.0
48	116616.0
49	124354.5
50	132093.0
51	132662.0
52	133231.0
53	133231.0
54	126799.5
55	120368.0
56	120368.0
57	117215.0
58	114062.0
59	102270.0
60	90478.0
61	90478.0
62	87086.0
63	83694.0
64	69153.0
65	54612.0
66	54612.0
67	46150.0
68	37688.0
69	37688.0
70	30507.0
71	23326.0
72	18131.5
73	12937.0
74	12937.0
75	9758.0
76	6579.0
77	6579.0
78	6585.5
79	6592.0
80	5166.5
81	3741.0
82	3741.0
83	3628.0
84	3515.0
85	3515.0
86	2213.0
87	911.0
88	765.5
89	620.0
90	620.0
91	367.0
92	114.0
93	77.0
94	40.0
95	40.0
96	26.5
97	13.0
98	13.0
99	19.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009883620370141583
2	0.0
3	0.0
4	4.11817515422566E-4
5	0.0
6	0.0
7	6.863625257042766E-4
8	0.002814086355387534
9	0.00439272016450737
10	3.431812628521383E-4
11	0.00555953645820464
12	4.11817515422566E-4
13	1.372725051408553E-4
14	0.0
15	0.0
16	6.863625257042765E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	4.11817515422566E-4
24	0.0
25	6.863625257042765E-5
26	1.372725051408553E-4
27	1.372725051408553E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.863625257042765E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1456956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.182886258549075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.17320430880011	41.83669201429532
2	12.38991763751737	12.9308332566272
3	3.445363001712772	5.393669569133725
4	1.3569473112062596	2.8323770879808094
5	0.7129334405848619	1.860146231997795
6	0.41110129158750675	1.2871471163792088
7	0.2771011731341852	1.012195729984019
8	0.19899342584403318	0.8307241045614563
9	0.13516387171731817	0.634791684968094
>10	0.7017351129728037	6.668043275671655
>50	0.07979024323016884	2.9890079672682806
>100	0.09686889479300524	11.181174297200771
>500	0.014140447457337545	5.180466047762733
>1k	0.006739839442282383	5.3627316161688645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3973	0.27269183146230913	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2751	0.1888183308212465	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2314	0.15882428844796959	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2279	0.15642201960800464	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2255	0.15477474954631437	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2175	0.14928384934068015	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2115	0.1451656741864545	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1944	0.13342887499691136	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1941	0.1332229662392001	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1932	0.13260523996606624	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1787	0.12265298334335423	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1780	0.12217252957536122	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1775	0.1218293483125091	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1742	0.11956435197768497	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1667	0.1144166330349029	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1664	0.11421072427719163	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1614	0.11077891164867024	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1575	0.10810209779842357	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1567	0.10755300777786014	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1559	0.10700391775729673	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1522	0.1044643764121909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.863625257042766E-5
2	0.0	0.0	0.0	0.0	6.863625257042766E-5
3	0.0	0.0	0.0	0.0	6.863625257042766E-5
4	0.0	0.0	0.0	6.863625257042766E-5	6.863625257042766E-5
5	0.0	0.0	0.0	6.863625257042766E-5	1.3727250514085533E-4
6	0.0	0.0	0.0	6.863625257042766E-5	2.0590875771128297E-4
7	0.0	0.0	0.0	6.863625257042766E-5	2.0590875771128297E-4
8	0.0	0.0	0.0	6.863625257042766E-5	2.0590875771128297E-4
9	0.0	0.0	0.0	6.863625257042766E-5	2.0590875771128297E-4
10	0.0	0.0	0.0	6.863625257042766E-5	2.0590875771128297E-4
11	0.0	0.0	0.0	6.863625257042766E-5	2.0590875771128297E-4
12	0.0	0.0	0.0	6.863625257042766E-5	4.1181751542256593E-4
13	0.0	0.0	0.0	6.863625257042766E-5	6.863625257042766E-4
14	0.0	0.0	0.0	6.863625257042766E-5	6.863625257042766E-4
15	0.0	0.0	0.0	6.863625257042766E-5	7.549987782747042E-4
16	0.0	0.0	0.0	1.3727250514085533E-4	8.236350308451319E-4
17	0.0	0.0	0.0	1.3727250514085533E-4	8.922712834155596E-4
18	0.0	0.0	0.0	1.3727250514085533E-4	0.0010981800411268426
19	0.0	0.0	0.0	1.3727250514085533E-4	0.0010981800411268426
20	0.0	0.0	0.0	2.0590875771128297E-4	0.0010981800411268426
21	0.0	0.0	0.0	4.1181751542256593E-4	0.0011668162936972703
22	0.0	0.0	0.0	6.17726273133849E-4	0.0013040887988381255
23	0.0	0.0	0.0	0.0013727250514085532	0.0013040887988381255
24	0.0	0.0	0.0	0.0021963600822536853	0.0013040887988381255
25	0.0	0.0	0.0	0.002814086355387534	0.0013040887988381255
26	0.0	0.0	0.0	0.004255447659366515	0.0013727250514085532
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	65	2.0987378E-4	17.23308	5
CTTACTC	140	4.0017767E-11	17.14398	3
CTAGCGG	420	0.0	16.761276	29
GTAGGAC	560	0.0	16.572514	3
GTATAGA	145	6.91216E-11	16.551104	1
ACGAACG	225	0.0	16.354942	15
TAGCGGC	435	0.0	16.1833	30
TCTAGCG	435	0.0	16.1833	28
TGTAGGA	630	0.0	15.9999485	2
CGAACGA	230	0.0	15.999399	16
GCATATA	60	0.0024429394	15.999398	1
TAACGAA	235	0.0	15.658986	13
ATAACGA	235	0.0	15.658986	12
AAGACGG	545	0.0	15.56172	5
CCGATAA	260	0.0	15.38615	9
GTAATAA	115	3.347959E-7	15.303773	1
GATAACG	245	0.0	15.02036	11
ACGTTTT	320	0.0	14.999436	29
CGCAAGA	525	0.0	14.933286	2
ACCGTCG	475	0.0	14.823039	8
>>END_MODULE