FastQCFastQC Report
Thu 2 Feb 2017
SRR4062809_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062809_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600052
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38540.24086717181691594No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24360.15224505203580882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24090.15055760687777647No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23120.14449530390262316No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20680.12924579951151588No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20410.1275583543534835No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20000.12499593763202696No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19590.12243352091057041No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19180.11987110418911384No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19100.11937112043858575No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18370.11480876871501676No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18360.11474627074620075No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16320.101996685107734No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16280.10174669323246995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG4350.022.43531429
TCTAGCG4500.021.6874728
TAGCGGC4550.021.0975230
AGCGGCG5300.018.1120231
ACCGTCG3350.017.6688298
ATACCGT3650.017.530366
CAAGACG5350.017.3430334
AAGACGG5400.017.182455
TACCGTC3850.016.620737
GCCGGTC6150.016.1275148
TGCGTCA703.6967322E-416.00045210
GACGGAC5700.015.9974537
CGCCGGT5900.015.9974537
CGCAAGA5200.015.69765952
GTCCTAT6350.015.63911151
CGGTCCA6300.015.49250110
ACCTCTA6750.015.40688125
CTATTCC5550.015.2768484
CCGTCGT3700.015.1355649
TCTTGCG6350.015.1232672
Produced by FastQC (version 0.11.5)