##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062809_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1600052
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255565444123064	32.0	32.0	32.0	32.0	32.0
2	30.904708721966536	32.0	32.0	32.0	32.0	32.0
3	30.954131490726553	32.0	32.0	32.0	32.0	32.0
4	31.01789191851265	32.0	32.0	32.0	32.0	32.0
5	30.927066745330777	32.0	32.0	32.0	32.0	32.0
6	34.57120331089239	36.0	36.0	36.0	32.0	36.0
7	34.511792741735896	36.0	36.0	36.0	32.0	36.0
8	34.50173556859402	36.0	36.0	36.0	32.0	36.0
9	34.633915647741446	36.0	36.0	36.0	32.0	36.0
10	34.37150667603303	36.0	36.0	36.0	32.0	36.0
11	34.607020896820856	36.0	36.0	36.0	32.0	36.0
12	34.44530177769222	36.0	36.0	36.0	32.0	36.0
13	34.52376047778447	36.0	36.0	36.0	32.0	36.0
14	34.42481619347371	36.0	36.0	36.0	32.0	36.0
15	34.377823970720954	36.0	36.0	36.0	32.0	36.0
16	34.38374440330689	36.0	36.0	36.0	32.0	36.0
17	34.314564151665074	36.0	36.0	36.0	32.0	36.0
18	34.34044206063303	36.0	36.0	36.0	32.0	36.0
19	34.32798121561049	36.0	36.0	36.0	32.0	36.0
20	34.31559224325209	36.0	36.0	36.0	32.0	36.0
21	34.29352046058503	36.0	36.0	36.0	32.0	36.0
22	34.265227005122334	36.0	36.0	36.0	32.0	36.0
23	34.212601840440186	36.0	36.0	36.0	32.0	36.0
24	34.19796981598098	36.0	36.0	36.0	32.0	36.0
25	34.17812421096314	36.0	36.0	36.0	32.0	36.0
26	34.126694007444755	36.0	36.0	36.0	32.0	36.0
27	34.12968578521198	36.0	36.0	36.0	32.0	36.0
28	34.115781862089484	36.0	36.0	36.0	32.0	36.0
29	34.08742278375953	36.0	36.0	36.0	32.0	36.0
30	34.05694939914453	36.0	36.0	36.0	32.0	36.0
31	34.07635564344159	36.0	36.0	36.0	32.0	36.0
32	34.02314174789319	36.0	36.0	36.0	32.0	36.0
33	33.99400519483117	36.0	36.0	36.0	32.0	36.0
34	34.00616729956276	36.0	36.0	36.0	32.0	36.0
35	33.968041663645934	36.0	36.0	36.0	32.0	36.0
36	33.91080914870267	36.0	36.0	36.0	32.0	36.0
37	33.90771174936815	36.0	36.0	36.0	32.0	36.0
38	33.312086107201516	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	4.0
9	3.0
10	19.0
11	15.0
12	12.0
13	11.0
14	188.0
15	440.0
16	548.0
17	617.0
18	787.0
19	1046.0
20	1395.0
21	1935.0
22	2937.0
23	4356.0
24	6604.0
25	9732.0
26	14439.0
27	20954.0
28	29440.0
29	40269.0
30	54439.0
31	74232.0
32	103079.0
33	155495.0
34	347580.0
35	729470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.97643536934795	16.876137698340436	11.29729367807353	25.850133254238074
2	17.032387732283425	18.95913012374275	37.243078085251675	26.765404058722154
3	19.287108611989492	22.989828427772334	27.95016535914907	29.7728976010891
4	12.651600520246422	14.574212302290421	35.52819324367145	37.2459939337917
5	14.947776436148006	36.10919449715133	33.32914594848641	15.61388311821425
6	35.410231955831264	34.95274510857813	16.19609686221521	13.440926073375389
7	31.195173656856152	29.783282043333593	20.276028529072807	18.745515770737452
8	28.838511013155287	32.55554076426792	18.924077570512253	19.68187065206454
9	27.427756809643718	14.061399446391261	18.352023409312352	40.15882033465267
10	16.163210817722796	26.03864525971522	30.982706068157	26.815437854404983
11	38.463322104236845	20.76180773870163	21.564302723107982	19.21056743395354
12	25.172608835597387	23.294989703157178	28.07922475242265	23.453176708822784
13	29.67719029795703	18.764017131841673	25.150203646892038	26.408588923309257
14	23.75966317642235	19.49242696988789	24.500027501925135	32.24788235176462
15	25.40626672201079	26.859050014752878	22.01122968978951	25.723453573446818
16	26.38262097933569	25.41303614066231	23.18002825247209	25.024314627529908
17	24.37264491802377	25.71680756526718	24.746957615400582	25.16358990130847
18	25.568079542386275	24.13772413172395	25.85196305889177	24.44223326699801
19	25.831545735015425	24.756424103325685	24.97912385181185	24.43290630984704
20	26.038050010406593	23.84885249323884	24.742632099688304	25.370465396666265
21	27.20974231053615	23.82794701581113	24.11414363832847	24.848167035324252
22	26.172631626317948	23.846025474912295	24.73544105449811	25.245901844271646
23	24.436103619300017	23.642926567055962	25.519799736857728	26.40117007678629
24	25.044426956333133	24.76564507065496	24.93110149473346	25.25882647827845
25	25.199214892047657	24.143007163484977	24.957619172636235	25.700158771831127
26	24.923426205365292	25.11445438202135	25.381556279551234	24.580563133062128
27	25.72555779804701	24.470984875995917	24.501111627113325	25.302345698843748
28	24.793258259825507	24.300178193848158	25.452094230856975	25.454469315469364
29	24.62817872422878	24.698808359298578	25.464233590329364	25.208779326143276
30	24.630686576716442	24.870874677186695	25.643564108910272	24.854874637186594
31	25.260460450450168	24.59737930129285	24.437999818744903	25.70416042951208
32	25.02623560479397	24.572714345984966	24.30138912761536	26.0996609216057
33	24.584911556972312	24.188824301518842	25.15238452403275	26.07387961747609
34	25.575681128910066	24.42831926112796	25.13557571863257	24.860423891329404
35	26.128932244687725	24.177983805333962	25.167866202104534	24.52521774787378
36	24.665321096100367	25.061366393781288	24.700762649728063	25.572549860390282
37	25.871994179621677	24.684979523669035	24.657852760429428	24.78517353627986
38	24.856886957728292	24.501877860620176	25.096643101479476	25.54459208017206
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	119.0
2	182.0
3	182.0
4	457.0
5	732.0
6	732.0
7	914.5
8	1097.0
9	1088.0
10	1079.0
11	1079.0
12	1355.0
13	1631.0
14	2376.5
15	3122.0
16	3122.0
17	4580.5
18	6039.0
19	6039.0
20	7404.0
21	8769.0
22	9490.0
23	10211.0
24	10211.0
25	11859.5
26	13508.0
27	13508.0
28	17338.0
29	21168.0
30	26191.5
31	31215.0
32	31215.0
33	40251.5
34	49288.0
35	49288.0
36	55878.0
37	62468.0
38	72641.5
39	82815.0
40	82815.0
41	88811.5
42	94808.0
43	108563.5
44	122319.0
45	122319.0
46	126928.5
47	131538.0
48	131538.0
49	140231.5
50	148925.0
51	152659.5
52	156394.0
53	156394.0
54	149868.0
55	143342.0
56	143342.0
57	139096.5
58	134851.0
59	121584.5
60	108318.0
61	108318.0
62	102056.5
63	95795.0
64	79956.5
65	64118.0
66	64118.0
67	53225.0
68	42332.0
69	42332.0
70	33579.0
71	24826.0
72	19407.0
73	13988.0
74	13988.0
75	10505.0
76	7022.0
77	7022.0
78	6859.5
79	6697.0
80	5270.0
81	3843.0
82	3843.0
83	3763.5
84	3684.0
85	3684.0
86	2464.0
87	1244.0
88	1091.0
89	938.0
90	938.0
91	678.0
92	418.0
93	361.5
94	305.0
95	305.0
96	277.0
97	249.0
98	249.0
99	483.5
100	718.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12368348028689068
2	0.05799811506126051
3	0.01256209173201871
4	0.0025624167214565526
5	4.999837505281078E-4
6	3.7498781289608087E-4
7	0.0
8	6.874776569761482E-4
9	3.124898440800674E-4
10	6.874776569761482E-4
11	0.002624914690272566
12	0.0029374045343526336
13	0.01399954501478702
14	0.01024966688582621
15	0.022749260649028907
16	0.011999610012674588
17	0.019999350021124313
18	0.006249796881601348
19	0.008749715634241888
20	0.006562286725681415
21	0.006437290788049389
22	0.007812246102001685
23	0.009812181104114116
24	0.014687022671763169
25	0.01699944751795567
26	0.017999415019011884
27	0.009062205478321955
28	0.006812278600945469
29	0.009812181104114116
30	0.003499886253696755
31	0.006062302975153307
32	0.007937242039633712
33	0.00949969126003405
34	0.012999577513730804
35	0.016624459705059585
36	0.014687022671763169
37	0.009749683135298103
38	0.005812311099889254
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1600052.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.611865850182426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.12808509453562	39.03596296086091
2	13.172726099121522	13.333924924198707
3	4.208757385512016	6.390391925744961
4	1.8234574570050663	3.691543367898208
5	0.973609040935871	2.4638085085185533
6	0.5934966146793276	1.802278262681252
7	0.3878124383641923	1.3739537773864448
8	0.2787643899426285	1.1287028726067403
9	0.19916345515621714	0.9072030667162756
>10	1.050817953513659	9.413465168382631
>50	0.08342868667654223	2.93984142201397
>100	0.08116928488556385	8.788651267157725
>500	0.012887803747425989	4.303577709823361
>1k	0.005824295924317514	4.426694766010359
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3854	0.24086717181691594	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2436	0.15224505203580882	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2409	0.15055760687777647	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2312	0.14449530390262316	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2068	0.12924579951151588	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2041	0.1275583543534835	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2000	0.12499593763202696	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1959	0.12243352091057041	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1918	0.11987110418911384	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1910	0.11937112043858575	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1837	0.11480876871501676	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1836	0.11474627074620075	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1632	0.101996685107734	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1628	0.10174669323246995	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2499593763202696E-4
2	0.0	0.0	0.0	6.249796881601348E-5	1.2499593763202696E-4
3	0.0	0.0	0.0	6.249796881601348E-5	1.2499593763202696E-4
4	0.0	0.0	0.0	6.249796881601348E-5	1.2499593763202696E-4
5	0.0	0.0	0.0	1.8749390644804044E-4	1.2499593763202696E-4
6	0.0	0.0	0.0	1.8749390644804044E-4	1.2499593763202696E-4
7	0.0	0.0	0.0	2.499918752640539E-4	1.2499593763202696E-4
8	0.0	0.0	0.0	2.499918752640539E-4	1.2499593763202696E-4
9	0.0	0.0	0.0	3.7498781289608087E-4	1.2499593763202696E-4
10	0.0	0.0	0.0	3.7498781289608087E-4	2.499918752640539E-4
11	0.0	0.0	0.0	3.7498781289608087E-4	3.124898440800674E-4
12	0.0	0.0	0.0	4.3748578171209435E-4	4.999837505281078E-4
13	0.0	0.0	0.0	4.3748578171209435E-4	5.624817193441214E-4
14	0.0	0.0	0.0	4.999837505281078E-4	5.624817193441214E-4
15	0.0	0.0	0.0	4.999837505281078E-4	5.624817193441214E-4
16	0.0	0.0	0.0	6.249796881601348E-4	6.249796881601348E-4
17	0.0	0.0	0.0	6.874776569761483E-4	6.249796881601348E-4
18	0.0	0.0	0.0	8.124735946081753E-4	7.499756257921617E-4
19	0.0	0.0	0.0	8.124735946081753E-4	7.499756257921617E-4
20	0.0	0.0	0.0	8.749715634241887E-4	0.001062465469872229
21	0.0	0.0	0.0	0.0012499593763202696	0.001062465469872229
22	0.0	0.0	0.0	0.0016249471892163506	0.0011249634386882427
23	0.0	0.0	0.0	0.0020624329709284447	0.0011249634386882427
24	0.0	0.0	0.0	0.0025624167214565526	0.0011249634386882427
25	0.0	0.0	0.0	0.002999902503168647	0.0011249634386882427
26	0.0	0.0	0.0	0.003874874066592836	0.0011874614075042561
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	435	0.0	22.435314	29
TCTAGCG	450	0.0	21.68747	28
TAGCGGC	455	0.0	21.09752	30
AGCGGCG	530	0.0	18.11202	31
ACCGTCG	335	0.0	17.668829	8
ATACCGT	365	0.0	17.53036	6
CAAGACG	535	0.0	17.343033	4
AAGACGG	540	0.0	17.18245	5
TACCGTC	385	0.0	16.62073	7
GCCGGTC	615	0.0	16.127514	8
TGCGTCA	70	3.6967322E-4	16.000452	10
GACGGAC	570	0.0	15.997453	7
CGCCGGT	590	0.0	15.997453	7
CGCAAGA	520	0.0	15.6976595	2
GTCCTAT	635	0.0	15.6391115	1
CGGTCCA	630	0.0	15.492501	10
ACCTCTA	675	0.0	15.406881	25
CTATTCC	555	0.0	15.276848	4
CCGTCGT	370	0.0	15.135564	9
TCTTGCG	635	0.0	15.123267	2
>>END_MODULE