FastQCFastQC Report
Thu 2 Feb 2017
SRR4062809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600052
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39210.24505453572758887No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26880.16799454017744422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25880.16174474329584287No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23660.14787019421868788No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22480.1404954338983983No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20770.12980828123086No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20400.12749585638466748No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20310.12693337466532337No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20120.12574591325781914No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18860.11787116918700143No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18350.11468377277738474No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17300.10812148605170331No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16540.1033716404216863No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16490.10305915057760623No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16460.10287165667115818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16140.10087172166904576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5000.020.15925228
CTAGCGG5150.019.26142129
TAGCGGC5200.019.07621630
ACCGTCG4750.018.191648
GTATTAT1159.422365E-1018.087981
TACCGTC5150.017.7108487
ATACCGT5100.017.2569586
TCGCGTA2700.017.1861579
GTATTAG2650.016.9066161
GCGTAAC2750.016.87315611
CGTCGTA5300.016.60419510
CGCGTAA2700.016.59301410
AGCGGCG6300.016.50732431
CCGTCGT5250.016.4580769
GTCGTAG5250.016.1527911
TCAGATA5350.016.1499422
TCGTTTA4650.015.48329530
ATCGTTT4550.015.47195229
TTCGGAA6300.015.23753131
TTTTCGG6300.015.23753129
Produced by FastQC (version 0.11.5)