##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062809_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1600052
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.156723031501475	32.0	32.0	32.0	32.0	32.0
2	31.211294382932554	32.0	32.0	32.0	32.0	32.0
3	31.325118183659033	32.0	32.0	32.0	32.0	32.0
4	31.438914485279227	32.0	32.0	32.0	32.0	32.0
5	31.330976118276155	32.0	32.0	32.0	32.0	32.0
6	34.864795644141566	36.0	36.0	36.0	32.0	36.0
7	34.89785331976711	36.0	36.0	36.0	36.0	36.0
8	34.81541912387847	36.0	36.0	36.0	32.0	36.0
9	34.95111658871087	36.0	36.0	36.0	32.0	36.0
10	34.78002902405672	36.0	36.0	36.0	32.0	36.0
11	34.96780417136443	36.0	36.0	36.0	36.0	36.0
12	34.85532720186594	36.0	36.0	36.0	32.0	36.0
13	34.92495618892386	36.0	36.0	36.0	32.0	36.0
14	34.86247009472192	36.0	36.0	36.0	32.0	36.0
15	34.837175291803014	36.0	36.0	36.0	32.0	36.0
16	34.84158827338111	36.0	36.0	36.0	32.0	36.0
17	34.80718939134478	36.0	36.0	36.0	32.0	36.0
18	34.81090052073308	36.0	36.0	36.0	32.0	36.0
19	34.80855497196341	36.0	36.0	36.0	32.0	36.0
20	34.789611837615276	36.0	36.0	36.0	32.0	36.0
21	34.78654568726516	36.0	36.0	36.0	32.0	36.0
22	34.766948199183524	36.0	36.0	36.0	32.0	36.0
23	34.70695014912015	36.0	36.0	36.0	32.0	36.0
24	34.68086974673323	36.0	36.0	36.0	32.0	36.0
25	34.65776924749946	36.0	36.0	36.0	32.0	36.0
26	34.59155952431546	36.0	36.0	36.0	32.0	36.0
27	34.57443570583956	36.0	36.0	36.0	32.0	36.0
28	34.54913527810346	36.0	36.0	36.0	32.0	36.0
29	34.506044178564196	36.0	36.0	36.0	32.0	36.0
30	34.487323536985045	36.0	36.0	36.0	32.0	36.0
31	34.48300617729924	36.0	36.0	36.0	32.0	36.0
32	34.46040816173474	36.0	36.0	36.0	32.0	36.0
33	34.42668738266006	36.0	36.0	36.0	32.0	36.0
34	34.4184351508576	36.0	36.0	36.0	32.0	36.0
35	34.38357878368953	36.0	36.0	36.0	32.0	36.0
36	34.34966301095214	36.0	36.0	36.0	32.0	36.0
37	34.34656373667856	36.0	36.0	36.0	32.0	36.0
38	33.89101666695832	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	24.0
22	117.0
23	364.0
24	1157.0
25	2712.0
26	5678.0
27	10993.0
28	19195.0
29	30197.0
30	46363.0
31	67341.0
32	97748.0
33	157873.0
34	379364.0
35	780919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21146303732987	18.15941372251824	12.03412659968436	26.59499664046752
2	15.973803335018696	20.06192302620354	36.42227653993529	27.541997098842476
3	18.257844120066096	23.714729271298683	28.291455527695348	29.735971080939873
4	12.076497609449705	15.515702634292678	35.55638886284804	36.85141089340958
5	14.45196781104614	36.57899868254282	32.95955381450103	16.009479691910013
6	35.51024311100084	35.26893830259161	15.796059000619355	13.424759585788204
7	31.020502548365826	30.04034261536394	20.14424594308285	18.79490889318738
8	28.71678462411532	32.82631630262773	18.592485556358326	19.864413516898622
9	27.317221952428362	13.88967535361225	18.085959448420518	40.707143245538866
10	15.843706016614625	26.383593869137943	30.983865363029334	26.7888347512181
11	38.6041601692018	20.771220694219878	21.355226899285803	19.26939223729252
12	24.941767335584515	23.46506071384153	27.913805031977184	23.679366918596767
13	29.655459905527714	18.751648390108784	25.0682792869705	26.524612417393
14	23.665980855622195	19.350933594658173	24.468767265063885	32.514318284655744
15	25.431673470612203	26.92143755327952	21.787104419106377	25.8597845570019
16	26.37055092028374	25.461266835411394	22.948595356395113	25.21958688790975
17	24.411394129690787	25.81241109663936	24.5761387754898	25.20005599818006
18	25.658041113663803	24.116466214848018	25.78384952489044	24.441643146597734
19	25.905783062050485	24.772257401634448	24.841630147020222	24.480329389294848
20	26.10008924709947	23.75741538400002	24.670385712464345	25.472109656436164
21	27.25166541774658	23.856581892179552	24.049950970251537	24.84180171982233
22	26.12680853723321	23.909627824130496	24.699478141308084	25.26408549732821
23	24.38669065759861	23.55102874974454	25.491406825665962	26.57087376699089
24	25.06549787131918	24.71032191453778	24.848317429683537	25.375862784459507
25	25.196884349311365	24.116606283174725	24.99451579980888	25.69199356770503
26	25.038373752853033	25.087372160404787	25.26574136340569	24.608512723336492
27	25.752866627376253	24.266539004069244	24.506968840368213	25.47362552818629
28	24.797444083067298	24.13696554861967	25.562606715281756	25.502983653031276
29	24.691510025924156	24.534827618102412	25.4019869354246	25.371675420548833
30	24.681197861069514	24.633324416956448	25.6462914955264	25.03918622644764
31	25.37892518493149	24.437830770499957	24.437080794874166	25.746163249694387
32	25.076247521955537	24.46382992552742	24.396519613112574	26.06340293940447
33	24.595388149885128	24.08034238887236	25.14687022671763	26.177399234524877
34	25.533795151657575	24.257586628434577	25.182681562849208	25.025936657058644
35	26.102526667883293	24.129528290330565	25.106059053080777	24.661885988705368
36	24.71950911595373	24.91894013444563	24.768195033661407	25.593355715939232
37	26.056590660803526	24.55526445390525	24.583076050028374	24.80506883526285
38	24.936970719665013	24.214493297084466	25.06196681353708	25.786569169713445
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	48.0
2	63.0
3	63.0
4	294.0
5	525.0
6	525.0
7	712.0
8	899.0
9	948.0
10	997.0
11	997.0
12	1317.5
13	1638.0
14	2350.5
15	3063.0
16	3063.0
17	4587.5
18	6112.0
19	6112.0
20	7442.0
21	8772.0
22	9449.5
23	10127.0
24	10127.0
25	11757.0
26	13387.0
27	13387.0
28	17354.0
29	21321.0
30	26183.0
31	31045.0
32	31045.0
33	40211.0
34	49377.0
35	49377.0
36	56305.0
37	63233.0
38	72934.5
39	82636.0
40	82636.0
41	88560.5
42	94485.0
43	108117.5
44	121750.0
45	121750.0
46	126330.5
47	130911.0
48	130911.0
49	140037.5
50	149164.0
51	151893.0
52	154622.0
53	154622.0
54	149630.5
55	144639.0
56	144639.0
57	140262.0
58	135885.0
59	122924.5
60	109964.0
61	109964.0
62	103364.0
63	96764.0
64	80398.0
65	64032.0
66	64032.0
67	53431.5
68	42831.0
69	42831.0
70	34056.5
71	25282.0
72	19641.0
73	14000.0
74	14000.0
75	10516.0
76	7032.0
77	7032.0
78	6908.0
79	6784.0
80	5277.0
81	3770.0
82	3770.0
83	3509.5
84	3249.0
85	3249.0
86	2058.0
87	867.0
88	724.5
89	582.0
90	582.0
91	351.5
92	121.0
93	85.0
94	49.0
95	49.0
96	33.5
97	18.0
98	18.0
99	20.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007937242039633712
2	6.249796881601348E-5
3	0.0
4	1.2499593763202696E-4
5	0.0
6	6.249796881601348E-5
7	4.3748578171209435E-4
8	0.002499918752640539
9	0.004062367973040876
10	9.999675010562157E-4
11	0.00431235984830493
12	3.124898440800674E-4
13	3.7498781289608087E-4
14	0.0
15	0.0
16	1.2499593763202696E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.8749390644804044E-4
22	1.2499593763202696E-4
23	1.8749390644804044E-4
24	0.0
25	6.249796881601348E-5
26	0.0
27	6.249796881601348E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.2499593763202696E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1600052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.80004646988556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.51055067386272	36.84918381851149
2	14.147119940190134	13.807602209926461
3	4.515525033102289	6.610734943539696
4	1.902176137501706	3.713051356159627
5	1.0454412097522487	2.5508789808721555
6	0.6307465693531301	1.8468277137092186
7	0.4172742627670303	1.425410238960282
8	0.30625596121191745	1.195624411105683
9	0.22008492366917082	0.9666139052139077
>10	1.1083745407700845	9.63761476878591
>50	0.0896507548101767	3.0517346430777277
>100	0.08745230456010704	9.294871815611975
>500	0.01341439732486928	4.549757218172338
>1k	0.005933291124461412	4.500093976353655
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3921	0.24505453572758887	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2688	0.16799454017744422	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2588	0.16174474329584287	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2366	0.14787019421868788	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2248	0.1404954338983983	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2077	0.12980828123086	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2040	0.12749585638466748	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2031	0.12693337466532337	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2012	0.12574591325781914	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1886	0.11787116918700143	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1835	0.11468377277738474	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1730	0.10812148605170331	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1654	0.1033716404216863	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1649	0.10305915057760623	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1646	0.10287165667115818	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1614	0.10087172166904576	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.249796881601348E-5	6.249796881601348E-5
6	0.0	0.0	0.0	6.249796881601348E-5	6.249796881601348E-5
7	0.0	0.0	0.0	6.249796881601348E-5	6.249796881601348E-5
8	0.0	0.0	0.0	6.249796881601348E-5	6.249796881601348E-5
9	0.0	0.0	0.0	1.2499593763202696E-4	6.249796881601348E-5
10	0.0	0.0	0.0	1.2499593763202696E-4	6.249796881601348E-5
11	0.0	0.0	0.0	1.2499593763202696E-4	6.249796881601348E-5
12	0.0	0.0	0.0	1.8749390644804044E-4	3.124898440800674E-4
13	0.0	0.0	0.0	1.8749390644804044E-4	3.7498781289608087E-4
14	0.0	0.0	0.0	1.8749390644804044E-4	3.7498781289608087E-4
15	0.0	0.0	0.0	1.8749390644804044E-4	5.624817193441214E-4
16	0.0	0.0	0.0	3.124898440800674E-4	5.624817193441214E-4
17	0.0	0.0	0.0	3.124898440800674E-4	5.624817193441214E-4
18	0.0	0.0	0.0	3.7498781289608087E-4	6.249796881601348E-4
19	0.0	0.0	0.0	3.7498781289608087E-4	6.249796881601348E-4
20	0.0	0.0	0.0	3.7498781289608087E-4	8.124735946081753E-4
21	0.0	0.0	0.0	8.124735946081753E-4	9.374695322402022E-4
22	0.0	0.0	0.0	0.0011249634386882427	9.374695322402022E-4
23	0.0	0.0	0.0	0.00143745328276831	9.374695322402022E-4
24	0.0	0.0	0.0	0.0019999350021124313	9.374695322402022E-4
25	0.0	0.0	0.0	0.002499918752640539	9.374695322402022E-4
26	0.0	0.0	0.0	0.003249894378432701	9.999675010562157E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	500	0.0	20.159252	28
CTAGCGG	515	0.0	19.261421	29
TAGCGGC	520	0.0	19.076216	30
ACCGTCG	475	0.0	18.19164	8
GTATTAT	115	9.422365E-10	18.08798	1
TACCGTC	515	0.0	17.710848	7
ATACCGT	510	0.0	17.256958	6
TCGCGTA	270	0.0	17.186157	9
GTATTAG	265	0.0	16.906616	1
GCGTAAC	275	0.0	16.873156	11
CGTCGTA	530	0.0	16.604195	10
CGCGTAA	270	0.0	16.593014	10
AGCGGCG	630	0.0	16.507324	31
CCGTCGT	525	0.0	16.458076	9
GTCGTAG	525	0.0	16.15279	11
TCAGATA	535	0.0	16.149942	2
TCGTTTA	465	0.0	15.483295	30
ATCGTTT	455	0.0	15.471952	29
TTCGGAA	630	0.0	15.237531	31
TTTTCGG	630	0.0	15.237531	29
>>END_MODULE