FastQCFastQC Report
Thu 2 Feb 2017
SRR4062808_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062808_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510119
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26000.5096849950697778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17030.3338436717707045No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16880.3309031814145327No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14160.2775822896226175No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13760.2697409820061593No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12930.2534702687020087No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12910.2530782033211858No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12670.24837341875131097No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12650.24798135337048807No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12500.24504086301431627No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12490.24484483032390483No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12120.23759162077868104No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12000.2352392284937436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10410.20407003071832258No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10340.20269780188544242No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10330.20250176919503096No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9650.18917154624705215No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9570.18760328472376053No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT9480.18583899051005745No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9020.17682148675113063No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8990.17623338867989627No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8670.16996034258672976No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT8510.16682381954014652No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT8400.16466745994562051No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT8380.16427539456479762No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT8200.16074680613739148No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC8110.1589825119236884No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG7840.15368962928257918No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.15212136775928756No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC7700.1509451716168188No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT7690.15074913892640734No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT7640.14976897547435009No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG7640.14976897547435009No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT7620.1493769100935272No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC7570.14839674664146993No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC7230.14173163516748052No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT6960.1364387525263713No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG6930.13585065445513694No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG6920.1356546217647255No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC6680.13094983719485062No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT6630.12996967374279336No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6620.1297736410523819No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC6590.12918554298114754No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG6570.12879347760032464No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6350.12448075841127267No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA6330.12408869303044975No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC6330.12408869303044975No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC6050.11859977769892907No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA5940.11644341810440309No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC5930.11624738541399164No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA5840.11448309120028856No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG5740.11252276429617404No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC5690.11154260084411675No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG5470.10722988165506481No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTC5420.10624971820300753No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGC5400.10585765282218464No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC5350.10487748937012738No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC5320.10428939129889299No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA5310.10409335860848154No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT5280.10350526053724718No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG5220.10232906439477847No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT5180.10154493363313266No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA5180.10154493363313266No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT5110.10017270480025249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC552.216102E-623.2708033
ATCGACA350.002064790622.85749215
CTTGCAC350.00206596822.8552513
CATCGTT2600.022.76734728
GCATCGT2650.021.7340527
ATCGTTT2750.021.52549229
GACTATA453.4953986E-421.367181
GGCATCG2700.021.33156826
CTACACC453.539705E-421.3273874
CTTCTAC400.00443919620.031731
GCGCCAC400.00447972220.00030713
CGACAAT400.00447972220.00030717
TCGACAA400.00447972220.00030716
CCTACGT400.00448226519.99834632
TCGGAGA400.00448226519.99834632
CACCATT400.00448226519.9983467
TACACCA659.439662E-619.686825
CGGCATC2950.019.52380825
ATTCGTT507.1924325E-419.19841232
AATGTAT507.1971794E-419.1965316
Produced by FastQC (version 0.11.5)