##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062808_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	510119
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19104365844048	32.0	32.0	32.0	32.0	32.0
2	30.883727130336254	32.0	32.0	32.0	32.0	32.0
3	30.944420811614545	32.0	32.0	32.0	32.0	32.0
4	30.990639439032854	32.0	32.0	32.0	32.0	32.0
5	30.966356869671586	32.0	32.0	32.0	32.0	32.0
6	34.599070020916685	36.0	36.0	36.0	32.0	36.0
7	34.481669963283075	36.0	36.0	36.0	32.0	36.0
8	34.47156251776546	36.0	36.0	36.0	32.0	36.0
9	34.54082282761473	36.0	36.0	36.0	32.0	36.0
10	34.34235541118837	36.0	36.0	36.0	32.0	36.0
11	34.57736724176124	36.0	36.0	36.0	32.0	36.0
12	34.398244331224674	36.0	36.0	36.0	32.0	36.0
13	34.49446501698623	36.0	36.0	36.0	32.0	36.0
14	34.39751704994325	36.0	36.0	36.0	32.0	36.0
15	34.359631772194334	36.0	36.0	36.0	32.0	36.0
16	34.37308549573727	36.0	36.0	36.0	32.0	36.0
17	34.27756660700739	36.0	36.0	36.0	32.0	36.0
18	34.309302339258096	36.0	36.0	36.0	32.0	36.0
19	34.28326919797146	36.0	36.0	36.0	32.0	36.0
20	34.252248985040744	36.0	36.0	36.0	32.0	36.0
21	34.18930484847653	36.0	36.0	36.0	32.0	36.0
22	34.170334765025416	36.0	36.0	36.0	32.0	36.0
23	34.13551935920834	36.0	36.0	36.0	32.0	36.0
24	34.11340099074922	36.0	36.0	36.0	32.0	36.0
25	34.09217065037766	36.0	36.0	36.0	32.0	36.0
26	34.04701452014138	36.0	36.0	36.0	32.0	36.0
27	34.02016588286263	36.0	36.0	36.0	32.0	36.0
28	33.998521913514296	36.0	36.0	36.0	32.0	36.0
29	33.95991719579157	36.0	36.0	36.0	32.0	36.0
30	33.90466734232601	36.0	36.0	36.0	32.0	36.0
31	33.92814421732968	36.0	36.0	36.0	32.0	36.0
32	33.85751951995515	36.0	36.0	36.0	32.0	36.0
33	33.818920683213136	36.0	36.0	36.0	32.0	36.0
34	33.81644283000633	36.0	36.0	36.0	32.0	36.0
35	33.74761183174906	36.0	36.0	36.0	27.0	36.0
36	33.69318335525632	36.0	36.0	36.0	27.0	36.0
37	33.66916739035401	36.0	36.0	36.0	27.0	36.0
38	33.014651483281355	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	2.0
12	1.0
13	1.0
14	122.0
15	229.0
16	274.0
17	346.0
18	397.0
19	507.0
20	647.0
21	936.0
22	1347.0
23	1725.0
24	2406.0
25	3507.0
26	4946.0
27	6861.0
28	9831.0
29	13233.0
30	17825.0
31	23773.0
32	32531.0
33	48224.0
34	109323.0
35	231119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.21465360216067	18.38157936059293	11.674243137993844	24.72952389925256
2	15.995833978961985	21.0758690488566	38.51215775960647	24.41613921257495
3	18.17602553470143	26.447943440950024	28.95122252480634	26.424808499542202
4	11.38671993695391	16.271615732508923	37.59848501185055	34.743179318686614
5	13.630010370169982	37.78211665167011	33.291578206568296	15.296294771591615
6	31.784276940388185	37.399851406638085	17.86158861594497	12.95428303702876
7	28.870126382275508	31.707111477910054	21.79158196420835	17.631180175606083
8	27.720002587631516	32.5414266083012	20.44091672727344	19.297654076793847
9	28.154074155391495	14.0245982302134	19.601543172364043	38.219784442031056
10	16.803988104689708	25.357712054293422	30.554166985221038	27.28413285579583
11	37.21227019842623	21.29367161065958	23.08601606699679	18.408042123917397
12	25.779095138116997	24.79774009970379	28.37774523584083	21.045419526338385
13	29.15490306794655	19.374843152017064	26.179496831670747	25.290756948365644
14	23.851291873908245	21.973521437127626	24.601772687438856	29.573414001525272
15	24.747845114940276	28.524678237817742	23.218445345526703	23.50903130171528
16	25.053425296730957	25.651200451713336	25.854706719627963	23.44066753192774
17	22.919252344511303	26.318916279917886	26.343032933938016	24.4187984416328
18	24.705937705843606	23.337371788839747	29.35388632727957	22.602804178037076
19	25.342440240820718	25.360868397475734	26.29168635277373	23.005005008929814
20	25.439378939217207	23.58305805781276	26.582891422186062	24.39467158078397
21	28.065078328853758	23.719008102444064	24.001309565118518	24.21460400358366
22	25.932380825419727	25.296809203045722	25.263677924766515	23.507132046768035
23	22.451056121268678	24.745821194571768	26.92962197641911	25.873500707740444
24	24.31266677253843	26.228553615226712	26.155227614493455	23.303551997741405
25	24.07525047546223	24.851087190949553	26.39570221359528	24.677960119992942
26	22.600302333378426	26.7908154962522	27.860354133948196	22.748528036421174
27	24.9413333071939	25.594557769783467	25.66513424233216	23.798974680690474
28	22.534841275909187	25.256077741771694	27.387820795571056	24.821260186748063
29	22.790321979833863	25.40999837275003	27.290354328778992	24.50932531863711
30	22.69754640193841	25.759839092897973	28.206183788531057	23.336430716632556
31	24.559150746429587	25.230986384889086	25.111988943236064	25.097873925445256
32	23.132599089579568	26.015989209831915	25.4621697177383	25.38924198285022
33	22.26151804285902	25.306268881434878	27.102615023593827	25.329598052112267
34	24.001991899087567	25.577670340701008	27.636031415542302	22.784306344669123
35	25.82218074448187	25.144594801253223	26.758762935107818	22.274461519157096
36	22.54329933693295	26.676391127213982	26.35054327581001	24.429766260043053
37	24.886291454606766	26.590939389424324	25.029799480861676	23.49296967510724
38	22.88789343161568	26.4913398484596	26.22295846852057	24.39780825140415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	400.0
1	328.0
2	256.0
3	256.0
4	558.0
5	860.0
6	860.0
7	976.5
8	1093.0
9	1059.5
10	1026.0
11	1026.0
12	1232.0
13	1438.0
14	1737.0
15	2036.0
16	2036.0
17	2840.5
18	3645.0
19	3645.0
20	4260.5
21	4876.0
22	4948.0
23	5020.0
24	5020.0
25	5846.5
26	6673.0
27	6673.0
28	8823.0
29	10973.0
30	14053.5
31	17134.0
32	17134.0
33	22052.5
34	26971.0
35	26971.0
36	27939.5
37	28908.0
38	31934.5
39	34961.0
40	34961.0
41	33549.5
42	32138.0
43	35897.0
44	39656.0
45	39656.0
46	39244.0
47	38832.0
48	38832.0
49	39855.5
50	40879.0
51	39626.0
52	38373.0
53	38373.0
54	35132.5
55	31892.0
56	31892.0
57	32543.5
58	33195.0
59	28794.0
60	24393.0
61	24393.0
62	26369.0
63	28345.0
64	23140.5
65	17936.0
66	17936.0
67	15725.5
68	13515.0
69	13515.0
70	11134.5
71	8754.0
72	6872.5
73	4991.0
74	4991.0
75	3738.0
76	2485.0
77	2485.0
78	2642.0
79	2799.0
80	2240.0
81	1681.0
82	1681.0
83	1871.0
84	2061.0
85	2061.0
86	1343.0
87	625.0
88	586.5
89	548.0
90	548.0
91	380.5
92	213.0
93	176.5
94	140.0
95	140.0
96	139.0
97	138.0
98	138.0
99	199.0
100	260.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1268331506962101
2	0.05488915331520684
3	0.013330222947978806
4	0.003136523046583248
5	3.92065380822906E-4
6	3.92065380822906E-4
7	0.0
8	0.0
9	1.96032690411453E-4
10	3.92065380822906E-4
11	9.80163452057265E-4
12	0.001568261523291624
13	0.013918321019213163
14	0.00901750375892684
15	0.022543759397317096
16	0.011957994115098635
17	0.019211203660322397
18	0.00450875187946342
19	0.00588098071234359
20	0.004704784569874872
21	0.005096849950697778
22	0.005292882641109231
23	0.008821471068515385
24	0.013330222947978806
25	0.017446909446619318
26	0.016858811375384957
27	0.006665111473989403
28	0.00392065380822906
29	0.01097783066304137
30	0.002156359594525983
31	0.004704784569874872
32	0.0049008172602863255
33	0.005488915331520685
34	0.010389732591807008
35	0.01431038640003607
36	0.012742124876744445
37	0.008429405687692479
38	0.004704784569874872
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	510119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.612432869953295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.92786934737198	32.02046788223658
2	10.039285351967457	7.752824634302978
3	2.5705973241717825	2.977710498457936
4	1.0274263666257812	1.5868572644063204
5	0.5549001380183668	1.0713022164381003
6	0.35541924034796407	0.8234160935175335
7	0.2668179942036633	0.7211744322779177
8	0.20399070244682638	0.6301261843458161
9	0.1490979598275471	0.5181331468399341
>10	1.3266877584084917	10.930701912259998
>50	0.2653191559107221	7.290360698059149
>100	0.2790425605267927	22.66180175532041
>500	0.025413712251984764	6.713561223226818
>1k	0.008132387920635125	4.301562058310495
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2600	0.5096849950697778	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1703	0.3338436717707045	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1688	0.3309031814145327	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1416	0.2775822896226175	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1376	0.2697409820061593	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1293	0.2534702687020087	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1291	0.2530782033211858	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1267	0.24837341875131097	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1265	0.24798135337048807	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1250	0.24504086301431627	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1249	0.24484483032390483	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1212	0.23759162077868104	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1200	0.2352392284937436	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1041	0.20407003071832258	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1034	0.20269780188544242	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1033	0.20250176919503096	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	965	0.18917154624705215	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	957	0.18760328472376053	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	948	0.18583899051005745	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	902	0.17682148675113063	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	899	0.17623338867989627	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	867	0.16996034258672976	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	851	0.16682381954014652	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	840	0.16466745994562051	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	838	0.16427539456479762	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	820	0.16074680613739148	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	811	0.1589825119236884	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	784	0.15368962928257918	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.15212136775928756	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	770	0.1509451716168188	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	769	0.15074913892640734	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	764	0.14976897547435009	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	764	0.14976897547435009	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	762	0.1493769100935272	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	757	0.14839674664146993	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	723	0.14173163516748052	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	696	0.1364387525263713	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	693	0.13585065445513694	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	692	0.1356546217647255	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	668	0.13094983719485062	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT	663	0.12996967374279336	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	662	0.1297736410523819	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	659	0.12918554298114754	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	657	0.12879347760032464	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	635	0.12448075841127267	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	633	0.12408869303044975	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	633	0.12408869303044975	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC	605	0.11859977769892907	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA	594	0.11644341810440309	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	593	0.11624738541399164	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA	584	0.11448309120028856	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	574	0.11252276429617404	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	569	0.11154260084411675	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	547	0.10722988165506481	No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTC	542	0.10624971820300753	No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGC	540	0.10585765282218464	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC	535	0.10487748937012738	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC	532	0.10428939129889299	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA	531	0.10409335860848154	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	528	0.10350526053724718	No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG	522	0.10232906439477847	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT	518	0.10154493363313266	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	518	0.10154493363313266	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT	511	0.10017270480025249	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.96032690411453E-4	0.0
9	0.0	0.0	0.0	1.96032690411453E-4	0.0
10	0.0	0.0	0.0	1.96032690411453E-4	0.0
11	0.0	0.0	0.0	1.96032690411453E-4	0.0
12	0.0	0.0	0.0	1.96032690411453E-4	0.0
13	0.0	0.0	0.0	1.96032690411453E-4	0.0
14	0.0	0.0	0.0	1.96032690411453E-4	0.0
15	0.0	0.0	0.0	1.96032690411453E-4	0.0
16	0.0	0.0	0.0	1.96032690411453E-4	0.0
17	0.0	0.0	0.0	1.96032690411453E-4	0.0
18	0.0	0.0	0.0	3.92065380822906E-4	0.0
19	0.0	0.0	0.0	3.92065380822906E-4	0.0
20	0.0	0.0	0.0	7.84130761645812E-4	0.0
21	0.0	0.0	0.0	0.0013722288328801712	0.0
22	0.0	0.0	0.0	0.002548424975348889	0.0
23	0.0	0.0	0.0	0.0049008172602863255	0.0
24	0.0	0.0	0.0	0.008233372997281026	0.0
25	0.0	0.0	0.0	0.009997667210984103	0.0
26	0.0	0.0	0.0	0.011957994115098633	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACAC	55	2.216102E-6	23.270803	3
ATCGACA	35	0.0020647906	22.857492	15
CTTGCAC	35	0.002065968	22.855251	3
CATCGTT	260	0.0	22.767347	28
GCATCGT	265	0.0	21.73405	27
ATCGTTT	275	0.0	21.525492	29
GACTATA	45	3.4953986E-4	21.36718	1
GGCATCG	270	0.0	21.331568	26
CTACACC	45	3.539705E-4	21.327387	4
CTTCTAC	40	0.004439196	20.03173	1
GCGCCAC	40	0.004479722	20.000307	13
CGACAAT	40	0.004479722	20.000307	17
TCGACAA	40	0.004479722	20.000307	16
CCTACGT	40	0.004482265	19.998346	32
TCGGAGA	40	0.004482265	19.998346	32
CACCATT	40	0.004482265	19.998346	7
TACACCA	65	9.439662E-6	19.68682	5
CGGCATC	295	0.0	19.523808	25
ATTCGTT	50	7.1924325E-4	19.198412	32
AATGTAT	50	7.1971794E-4	19.196531	6
>>END_MODULE