FastQCFastQC Report
Thu 2 Feb 2017
SRR4062808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510119
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29230.5730035540726772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21740.42617506895449886No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17870.35031041776526656No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17070.3346278025323503No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14390.28209104150208086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14250.27934658383632055No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13960.2736616358143884No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13940.27326957043356553No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13110.2569988571294149No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12810.2511178764170713No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12760.250137712965014No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12620.2473932552992537No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11880.23288683620880618No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11530.22602569204440534No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11060.21681215559506703No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11020.21602802483342123No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10350.20289383457585386No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10170.19936524614844772No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.19505252695939573No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9790.1919160039128125No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9590.18799535010458343No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9190.1801540424881253No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9120.17878181365524515No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG9070.1778016502031879No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT8630.16917621182508397No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT8320.1630991984223289No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT8320.1630991984223289No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC8160.15996267537574566No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT8060.1580023484716311No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT7940.1556499561866937No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG7830.1534935965921677No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC7800.15290549852093335No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG7620.1493769100935272No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG7160.14035940633460037No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC6990.13702685059760566No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC6980.1368308179071942No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT6900.13526255638390258No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC6840.13408636024143386No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC6810.1334982621701995No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6800.13330222947978804No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC6790.1331061967893766No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT6640.1301657064332048No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC6610.12957760836197044No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT6540.12820537952909028No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT6510.1276172814578559No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA6490.127225216077033No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC6480.12702918338662156No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG6370.12487282379209556No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC6140.12036407191263215No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC6120.11997200653180923No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA6110.11977597384139778No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG6000.1176196142468718No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA5950.11663945079481454No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC5880.11526722196193437No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC5860.11487515658111147No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGG5860.11487515658111147No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA5850.11467912389070002No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG5840.11448309120028856No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG5630.11036640470164805No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA5620.11017037201123658No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGT5550.10879814317835643No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT5480.10742591434547626No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA5420.10624971820300753No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG5350.10487748937012738No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTC5340.10468145667971591No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT5320.10428939129889299No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAG5210.10213303170436702No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG5190.10174096632354411No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC5130.1005647701810754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGCG252.8928622E-431.99990326
TCGGAGA308.410565E-426.66658632
CTCGGAG350.002065011522.85707331
TAATCCC350.002065011522.8570735
CTAGGAC653.808018E-722.1537783
GGACCTG3200.020.4999376
TTACCAA555.8252845E-520.36357517
TTACATT555.8252845E-520.3635754
AGGACCT3250.019.6922475
GGCATAC507.18384E-419.2018241
TAGGACA1003.250534E-919.1999424
CCCAATT852.3895882E-718.82347313
GTATTAG2000.018.4017491
AGGGGCG2200.018.18176332
ATTACAC450.00884387417.7777233
ACCCAAT904.3550972E-717.77772312
GGGTTAA550.001362368317.4562031
CTCTAGG550.001362368317.4562031
GAACTCG550.001363263817.45449331
ATTCGTT550.001363263817.45449332
Produced by FastQC (version 0.11.5)