##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062808_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	510119
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15166461159063	32.0	32.0	32.0	32.0	32.0
2	31.347665936771616	32.0	32.0	32.0	32.0	32.0
3	31.406967785947984	32.0	32.0	32.0	32.0	32.0
4	31.513778157645568	32.0	32.0	32.0	32.0	32.0
5	31.434592712680768	32.0	32.0	32.0	32.0	32.0
6	34.95163285429478	36.0	36.0	36.0	36.0	36.0
7	34.9788931602234	36.0	36.0	36.0	36.0	36.0
8	34.936134509790854	36.0	36.0	36.0	36.0	36.0
9	35.018070293402126	36.0	36.0	36.0	36.0	36.0
10	34.872288622850746	36.0	36.0	36.0	32.0	36.0
11	35.04803389013152	36.0	36.0	36.0	36.0	36.0
12	34.91301049362992	36.0	36.0	36.0	32.0	36.0
13	34.986242425786926	36.0	36.0	36.0	36.0	36.0
14	34.94015710059809	36.0	36.0	36.0	32.0	36.0
15	34.903242184666716	36.0	36.0	36.0	32.0	36.0
16	34.9028540399397	36.0	36.0	36.0	32.0	36.0
17	34.855167127670214	36.0	36.0	36.0	32.0	36.0
18	34.856025750854215	36.0	36.0	36.0	32.0	36.0
19	34.83553837437931	36.0	36.0	36.0	32.0	36.0
20	34.821402457073745	36.0	36.0	36.0	32.0	36.0
21	34.79148786851695	36.0	36.0	36.0	32.0	36.0
22	34.77566803040075	36.0	36.0	36.0	32.0	36.0
23	34.70693700881559	36.0	36.0	36.0	32.0	36.0
24	34.68222708818923	36.0	36.0	36.0	32.0	36.0
25	34.645308251604035	36.0	36.0	36.0	32.0	36.0
26	34.57962357802787	36.0	36.0	36.0	32.0	36.0
27	34.552280938369286	36.0	36.0	36.0	32.0	36.0
28	34.512266745602496	36.0	36.0	36.0	32.0	36.0
29	34.46014165322209	36.0	36.0	36.0	32.0	36.0
30	34.41895126431284	36.0	36.0	36.0	32.0	36.0
31	34.413803445862634	36.0	36.0	36.0	32.0	36.0
32	34.35964157382885	36.0	36.0	36.0	32.0	36.0
33	34.3140208461163	36.0	36.0	36.0	32.0	36.0
34	34.31297599187641	36.0	36.0	36.0	32.0	36.0
35	34.22634718565668	36.0	36.0	36.0	32.0	36.0
36	34.18287105557723	36.0	36.0	36.0	32.0	36.0
37	34.15780631578122	36.0	36.0	36.0	32.0	36.0
38	33.69072510531856	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	18.0
22	36.0
23	157.0
24	352.0
25	842.0
26	1927.0
27	3597.0
28	6273.0
29	10083.0
30	15249.0
31	21503.0
32	30822.0
33	48205.0
34	115563.0
35	255484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54427324823407	19.552376041766735	12.409644931784007	25.493705778215194
2	15.090596507873652	22.09504056896528	37.72962779273071	25.084735130430353
3	17.32595727663545	27.067213728561374	29.492726207022287	26.11410278778089
4	10.899831019489609	17.0989849407392	37.68245778427736	34.31872625549383
5	12.879347760032465	38.441814556995524	33.22714895936046	15.45168872361155
6	31.886677422326947	37.754916009793796	17.33497478039438	13.023431787484881
7	28.451316070626937	32.063232552532064	21.85714257709506	17.62830879974594
8	27.644145945564468	33.0543727994856	20.183568969708375	19.117912285241555
9	27.8535903533208	13.82073816464518	19.55703063891018	38.76864084312384
10	16.79301171705766	25.688526614795602	30.318695342778334	27.1997663253684
11	37.0581950421	21.34798419902175	23.12922102745567	18.464599731422577
12	25.360310203953613	25.01509460593277	28.43862964502192	21.185965545091705
13	29.34654334879381	19.310041990284603	26.067890174430232	25.275524486491364
14	23.662713994185673	22.14483287232979	24.62209798105932	29.570355152425222
15	24.67424267670877	28.811904673223303	23.014433886994993	23.499418763072928
16	24.809113167711846	25.681458640042816	25.732623172240203	23.77680502000514
17	22.851334688572667	26.63888230001235	26.152525195101532	24.35725781631345
18	24.802252023547446	23.187138687247487	29.308259445345108	22.702349843859963
19	25.530513468425998	25.188240390967596	26.22976207512365	23.05148406548276
20	25.48934660343959	23.413556444672714	26.709650101250887	24.387446850636813
21	27.949948933484148	23.784842360312005	24.221211129167898	24.043997577035945
22	25.72228386373349	25.170058692302565	25.49586566245457	23.611791781509375
23	22.44363068936991	24.430229868383392	27.274277716136268	25.85186172611043
24	24.405481858154666	25.90395574365981	26.256618553710016	23.433943844475504
25	24.029883223326323	24.649346525026512	26.61594647523421	24.704823776412955
26	22.744300839608016	26.687890472615212	27.904861414689513	22.662947273087262
27	25.055575376677552	25.256117212096026	25.785798579936408	23.902508831290014
28	22.639031284857065	24.973584594967058	27.676875395740996	24.710508724434888
29	23.020118835016927	24.84302682315303	27.640805380705284	24.496048961124757
30	22.91504531295639	25.13746792415103	28.470415726526554	23.477071036366024
31	24.770102662319967	24.755988308610345	25.522672160809535	24.951236868260153
32	23.257906488486018	25.713804033960702	25.80476320231162	25.22352627524166
33	22.376347479705714	24.93477012226559	27.50162217051316	25.18726022751554
34	24.01870935997287	25.07905018240842	28.003661890656883	22.898578566961827
35	25.867297630552873	24.89830804184906	26.851969834489598	22.38242449310847
36	22.879367363301505	26.117435343517887	26.527339699168234	24.475857594012375
37	25.12805835501128	26.14762437784125	25.293117880337725	23.431199386809745
38	23.2661398614833	25.851811048010365	26.532828614499753	24.34922047600658
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	176.0
1	153.0
2	130.0
3	130.0
4	465.0
5	800.0
6	800.0
7	913.5
8	1027.0
9	1101.0
10	1175.0
11	1175.0
12	1313.0
13	1451.0
14	1826.5
15	2202.0
16	2202.0
17	3125.0
18	4048.0
19	4048.0
20	4502.5
21	4957.0
22	5138.5
23	5320.0
24	5320.0
25	5849.0
26	6378.0
27	6378.0
28	8651.0
29	10924.0
30	13720.5
31	16517.0
32	16517.0
33	21974.0
34	27431.0
35	27431.0
36	28206.5
37	28982.0
38	31686.0
39	34390.0
40	34390.0
41	33040.5
42	31691.0
43	35349.5
44	39008.0
45	39008.0
46	39463.5
47	39919.0
48	39919.0
49	40800.0
50	41681.0
51	40137.0
52	38593.0
53	38593.0
54	35095.5
55	31598.0
56	31598.0
57	32653.5
58	33709.0
59	29116.5
60	24524.0
61	24524.0
62	26774.5
63	29025.0
64	23456.0
65	17887.0
66	17887.0
67	15721.0
68	13555.0
69	13555.0
70	11154.5
71	8754.0
72	6825.5
73	4897.0
74	4897.0
75	3565.5
76	2234.0
77	2234.0
78	2599.5
79	2965.0
80	2294.0
81	1623.0
82	1623.0
83	1667.0
84	1711.0
85	1711.0
86	1069.0
87	427.0
88	365.5
89	304.0
90	304.0
91	182.0
92	60.0
93	43.0
94	26.0
95	26.0
96	15.5
97	5.0
98	5.0
99	10.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009409569139749744
2	0.0
3	0.0
4	1.96032690411453E-4
5	0.0
6	0.0
7	3.92065380822906E-4
8	0.002156359594525983
9	0.003136523046583248
10	0.001568261523291624
11	0.004704784569874872
12	5.88098071234359E-4
13	1.96032690411453E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.96032690411453E-4
23	1.96032690411453E-4
24	0.0
25	0.0
26	0.0
27	1.96032690411453E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	510119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.11541650516613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.5794626880227	29.462762732455992
2	10.801177093928732	7.801780189865921
3	2.7839272307227234	3.0162807437287467
4	1.0897069202686567	1.574208771762576
5	0.6265970944449755	1.1314907523403603
6	0.36702772040324716	0.795321539478296
7	0.2794533120964693	0.7064800932078517
8	0.21844377370814297	0.6311350296343874
9	0.17312344074524316	0.5627182652389715
>10	1.4492314086947802	11.169489254519242
>50	0.2805193088246816	7.203487041354233
>100	0.31156707698585995	23.567823515822887
>500	0.029958372787101912	7.267027549147051
>1k	0.009804558366687898	5.109994521443485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2923	0.5730035540726772	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2174	0.42617506895449886	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1787	0.35031041776526656	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1707	0.3346278025323503	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1439	0.28209104150208086	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1425	0.27934658383632055	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1396	0.2736616358143884	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1394	0.27326957043356553	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1311	0.2569988571294149	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1281	0.2511178764170713	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1276	0.250137712965014	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1262	0.2473932552992537	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1188	0.23288683620880618	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1153	0.22602569204440534	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1106	0.21681215559506703	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1102	0.21602802483342123	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1035	0.20289383457585386	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1017	0.19936524614844772	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	995	0.19505252695939573	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	979	0.1919160039128125	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	959	0.18799535010458343	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	919	0.1801540424881253	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	912	0.17878181365524515	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	907	0.1778016502031879	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	863	0.16917621182508397	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	832	0.1630991984223289	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	832	0.1630991984223289	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	816	0.15996267537574566	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	806	0.1580023484716311	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	794	0.1556499561866937	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	783	0.1534935965921677	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	780	0.15290549852093335	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	762	0.1493769100935272	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	716	0.14035940633460037	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	699	0.13702685059760566	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	698	0.1368308179071942	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	690	0.13526255638390258	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC	684	0.13408636024143386	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	681	0.1334982621701995	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	680	0.13330222947978804	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	679	0.1331061967893766	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	664	0.1301657064332048	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	661	0.12957760836197044	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT	654	0.12820537952909028	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	651	0.1276172814578559	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	649	0.127225216077033	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	648	0.12702918338662156	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	637	0.12487282379209556	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	614	0.12036407191263215	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC	612	0.11997200653180923	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA	611	0.11977597384139778	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	600	0.1176196142468718	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	595	0.11663945079481454	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC	588	0.11526722196193437	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC	586	0.11487515658111147	No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGG	586	0.11487515658111147	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA	585	0.11467912389070002	No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG	584	0.11448309120028856	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	563	0.11036640470164805	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA	562	0.11017037201123658	No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGT	555	0.10879814317835643	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	548	0.10742591434547626	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA	542	0.10624971820300753	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG	535	0.10487748937012738	No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTC	534	0.10468145667971591	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT	532	0.10428939129889299	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAG	521	0.10213303170436702	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG	519	0.10174096632354411	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACC	513	0.1005647701810754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	1.96032690411453E-4	0.0
19	0.0	0.0	0.0	1.96032690411453E-4	0.0
20	0.0	0.0	0.0	3.92065380822906E-4	0.0
21	0.0	0.0	0.0	9.801634520572651E-4	0.0
22	0.0	0.0	0.0	0.002156359594525983	0.0
23	0.0	0.0	0.0	0.0049008172602863255	0.0
24	0.0	0.0	0.0	0.008233372997281026	0.0
25	0.0	0.0	0.0	0.009997667210984103	0.0
26	0.0	0.0	0.0	0.01176196142468718	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGGCG	25	2.8928622E-4	31.999903	26
TCGGAGA	30	8.410565E-4	26.666586	32
CTCGGAG	35	0.0020650115	22.857073	31
TAATCCC	35	0.0020650115	22.857073	5
CTAGGAC	65	3.808018E-7	22.153778	3
GGACCTG	320	0.0	20.499937	6
TTACCAA	55	5.8252845E-5	20.363575	17
TTACATT	55	5.8252845E-5	20.363575	4
AGGACCT	325	0.0	19.692247	5
GGCATAC	50	7.18384E-4	19.201824	1
TAGGACA	100	3.250534E-9	19.199942	4
CCCAATT	85	2.3895882E-7	18.823473	13
GTATTAG	200	0.0	18.401749	1
AGGGGCG	220	0.0	18.181763	32
ATTACAC	45	0.008843874	17.777723	3
ACCCAAT	90	4.3550972E-7	17.777723	12
GGGTTAA	55	0.0013623683	17.456203	1
CTCTAGG	55	0.0013623683	17.456203	1
GAACTCG	55	0.0013632638	17.454493	31
ATTCGTT	55	0.0013632638	17.454493	32
>>END_MODULE