FastQCFastQC Report
Thu 2 Feb 2017
SRR4062807_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062807_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050773
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32250.30691690783832476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21340.20308858335720464No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14030.13352075091385104No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13570.12914302137569197No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13240.12600247627223007No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13220.12581214020535358No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13190.12552663610503886No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13140.12505079593784768No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12680.12067306639968861No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12670.12057789836625037No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12280.11686634506215901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12230.11639050489496781No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12190.11600983276121483No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11550.10991907862116747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.10392349251455835No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10520.10011677117702873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAG350.002066136622.85768710
CGCGCTT555.8316116E-520.3641210
GTATCGA400.004482610620.0004759
AGTATCG400.004486316319.9976228
CGGCATC2900.018.75817525
TCTAGCG2900.018.20559728
CGAATGT1159.404175E-1018.08652723
GTCCGTA450.00880498417.7934461
ACGTCAC450.00884856817.77820231
AATCGGT450.008850994517.77735319
AACGTAT550.001364322417.4549631
CTAGCGG3050.017.31188829
GGCATCG3350.017.19280226
CATCGTT3100.017.03104228
TAATACT2200.016.723694
TATAAGA1354.4019544E-1016.5977292
TAGCGGC3200.016.50039330
ACGGGAT600.002441330316.0003831
CTATTCC3300.015.9965734
ATCGTTT3350.015.76156929
Produced by FastQC (version 0.11.5)