##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062807_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1050773
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.237762104660092	32.0	32.0	32.0	32.0	32.0
2	30.905080355129034	32.0	32.0	32.0	32.0	32.0
3	30.948012558373694	32.0	32.0	32.0	32.0	32.0
4	31.00439295642351	32.0	32.0	32.0	32.0	32.0
5	30.955701183795167	32.0	32.0	32.0	32.0	32.0
6	34.60484995332008	36.0	36.0	36.0	32.0	36.0
7	34.52067287606362	36.0	36.0	36.0	32.0	36.0
8	34.51581264459593	36.0	36.0	36.0	32.0	36.0
9	34.613307536451735	36.0	36.0	36.0	32.0	36.0
10	34.372188855252276	36.0	36.0	36.0	32.0	36.0
11	34.612887845424275	36.0	36.0	36.0	32.0	36.0
12	34.451069831447896	36.0	36.0	36.0	32.0	36.0
13	34.525242845029325	36.0	36.0	36.0	32.0	36.0
14	34.42671633169105	36.0	36.0	36.0	32.0	36.0
15	34.390547720582845	36.0	36.0	36.0	32.0	36.0
16	34.40143113688684	36.0	36.0	36.0	32.0	36.0
17	34.32960116028866	36.0	36.0	36.0	32.0	36.0
18	34.35304675700651	36.0	36.0	36.0	32.0	36.0
19	34.34039036023956	36.0	36.0	36.0	32.0	36.0
20	34.32244737921511	36.0	36.0	36.0	32.0	36.0
21	34.293731376805454	36.0	36.0	36.0	32.0	36.0
22	34.27859014268543	36.0	36.0	36.0	32.0	36.0
23	34.21852388670055	36.0	36.0	36.0	32.0	36.0
24	34.20413543172503	36.0	36.0	36.0	32.0	36.0
25	34.17649577977356	36.0	36.0	36.0	32.0	36.0
26	34.14413769672422	36.0	36.0	36.0	32.0	36.0
27	34.148020552488504	36.0	36.0	36.0	32.0	36.0
28	34.118313850850754	36.0	36.0	36.0	32.0	36.0
29	34.08063111633055	36.0	36.0	36.0	32.0	36.0
30	34.05604540657212	36.0	36.0	36.0	32.0	36.0
31	34.06966014543579	36.0	36.0	36.0	32.0	36.0
32	34.021597433508475	36.0	36.0	36.0	32.0	36.0
33	33.977738293618124	36.0	36.0	36.0	32.0	36.0
34	33.98242341590429	36.0	36.0	36.0	32.0	36.0
35	33.9288447647589	36.0	36.0	36.0	32.0	36.0
36	33.8961640620762	36.0	36.0	36.0	32.0	36.0
37	33.88743144332791	36.0	36.0	36.0	32.0	36.0
38	33.29160627461878	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	2.0
8	0.0
9	4.0
10	8.0
11	12.0
12	8.0
13	5.0
14	198.0
15	350.0
16	417.0
17	547.0
18	691.0
19	848.0
20	1106.0
21	1473.0
22	2035.0
23	3038.0
24	4543.0
25	6601.0
26	9814.0
27	13770.0
28	19351.0
29	26218.0
30	35762.0
31	47798.0
32	65084.0
33	97674.0
34	220761.0
35	492654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.35466382090775	17.56134976632063	11.61288785772476	25.471098555046858
2	16.379448251170267	20.088498440279263	38.08975154924642	25.442301759304048
3	18.934034357680414	24.379124220841707	28.797424078168532	27.889417343309354
4	12.27269742383276	15.56854314121287	36.67743043758393	35.48132899737044
5	14.389814717003038	36.86017219736746	33.5854339337496	15.164579151879906
6	33.29091987091334	36.225347769771986	17.14699833550159	13.336734023813083
7	29.517345342281676	30.88091422306647	21.173289733643454	18.4284507010084
8	27.701685430684165	33.204221666016345	19.950797986238662	19.14329491706082
9	27.6230764106322	14.188737782768825	19.003778181714363	39.18440762488461
10	15.966116244388633	26.500115629600796	31.66062188739866	25.87314623861191
11	37.331929894018764	21.292084145450115	22.503311913753198	18.872674046777927
12	24.732738265751383	24.05940905011568	29.069359095273946	22.138493588858996
13	29.46180248196776	19.475584846252396	25.596077737103307	25.466534934676545
14	23.54223277638448	20.261834171691262	25.236683433825245	30.959249618099015
15	25.10625578873922	27.541310061292812	22.72231852272803	24.630115627239938
16	25.3488779369802	25.905769168536928	24.52282102385752	24.222531870625343
17	23.49124186390955	25.98056666596863	25.942682325527382	24.58550914459444
18	24.530034757251247	24.86228438512405	27.221056879308968	23.38662397831574
19	25.468593258957377	24.902874089414844	25.78133773929331	23.84719491233447
20	25.46404203621731	24.399526797517495	25.713396522171134	24.42303464409406
21	26.91113627927782	24.209724852718637	24.58461421324628	24.29452465475726
22	25.415914784105016	24.758541005200364	25.398117057645596	24.427427153049027
23	23.93201277663353	24.339182310682347	26.007359127716846	25.72144578496728
24	24.422341093830312	25.475814281091164	25.7064400068531	24.395404618225427
25	24.75304634866486	24.49757377198977	25.8264309414983	24.922948937847064
26	23.90567487071415	25.843463461576903	26.48568075318467	23.765180914524276
27	24.92162323188293	25.063339088259763	25.55082640616583	24.464211273691483
28	23.90179648925114	25.19453401299685	26.117618842770074	24.786050654981935
29	23.92743200438583	25.033312520939297	26.09017604239637	24.949079432278502
30	23.81293183851381	25.545139615889756	26.497325694272604	24.144602851323828
31	24.677196004625422	25.09871849169375	25.10985376624489	25.114231737435937
32	24.13053037123893	25.35885668384255	25.261492836190797	25.249120108727723
33	23.839191388516117	24.755731947577306	26.038983905814273	25.3660927580923
34	24.54625837455373	25.209369791929397	26.23130567370959	24.01306615980728
35	25.372522032459273	25.050423135533773	25.969128381729075	23.607926450277887
36	23.938032839625325	25.853852150299677	25.49920475604428	24.708910254030712
37	25.03388294774031	25.56135288138303	25.28486422074931	24.119899950127348
38	24.133940289728724	25.450958259533817	25.787969433914498	24.62713201682296
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	340.0
1	316.0
2	292.0
3	292.0
4	640.0
5	988.0
6	988.0
7	1128.5
8	1269.0
9	1167.0
10	1065.0
11	1065.0
12	1452.0
13	1839.0
14	2361.5
15	2884.0
16	2884.0
17	4136.5
18	5389.0
19	5389.0
20	6385.5
21	7382.0
22	7907.5
23	8433.0
24	8433.0
25	10421.0
26	12409.0
27	12409.0
28	16078.0
29	19747.0
30	24427.5
31	29108.0
32	29108.0
33	36179.5
34	43251.0
35	43251.0
36	46940.0
37	50629.0
38	57147.0
39	63665.0
40	63665.0
41	65517.0
42	67369.0
43	74368.0
44	81367.0
45	81367.0
46	83900.5
47	86434.0
48	86434.0
49	89988.0
50	93542.0
51	93512.0
52	93482.0
53	93482.0
54	88025.5
55	82569.0
56	82569.0
57	79952.5
58	77336.0
59	68988.0
60	60640.0
61	60640.0
62	58203.0
63	55766.0
64	46281.0
65	36796.0
66	36796.0
67	30877.5
68	24959.0
69	24959.0
70	20296.0
71	15633.0
72	12462.5
73	9292.0
74	9292.0
75	7089.5
76	4887.0
77	4887.0
78	4631.0
79	4375.0
80	3423.0
81	2471.0
82	2471.0
83	2536.5
84	2602.0
85	2602.0
86	1697.0
87	792.0
88	708.0
89	624.0
90	624.0
91	453.0
92	282.0
93	239.0
94	196.0
95	196.0
96	178.0
97	160.0
98	160.0
99	334.5
100	509.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1197213860653062
2	0.05567329956137053
3	0.011420164012588828
4	0.002474368869394246
5	1.9033606687648047E-4
6	5.710082006294414E-4
7	9.516803343824024E-5
8	2.855041003147207E-4
9	2.855041003147207E-4
10	3.8067213375296094E-4
11	0.0019985287022030447
12	0.0022840328025177654
13	0.014751045182927235
14	0.010278147611329945
15	0.023030664092054134
16	0.011324995979150588
17	0.019699782921715727
18	0.005424577905979693
19	0.007137602507868017
20	0.005710082006294414
21	0.0059955861066091345
22	0.007327938574744498
23	0.009992643511015224
24	0.014560709116050754
25	0.01627373371793908
26	0.018462598487018603
27	0.007518274641620978
28	0.005424577905979693
29	0.011134659912274107
30	0.0031405451034619276
31	0.005519745939417934
32	0.007137602507868017
33	0.007898946775373939
34	0.011420164012588828
35	0.015607557483871397
36	0.014465541082612514
37	0.009231299243509301
38	0.006281090206923855
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1050773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.24325071898299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.63628551439793	44.28217502923967
2	11.313693997636038	12.273830801432492
3	3.0007135932077853	4.883053793166907
4	1.2616020389448672	2.7373358282426636
5	0.6402614135895558	1.7364930191514367
6	0.440768537784557	1.4345230982452348
7	0.2804537456552654	1.0648905988459512
8	0.20366972215159146	0.8838166242027503
9	0.14045565818699676	0.6856894333743339
>10	0.8707905144869204	8.855129359879598
>50	0.09632717721457833	3.6890805606629757
>100	0.10142018100845401	11.328217599633405
>500	0.010388525173864127	3.7598358217153702
>1k	0.003169380561517869	2.385928432207277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3225	0.30691690783832476	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2134	0.20308858335720464	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1403	0.13352075091385104	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1357	0.12914302137569197	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1324	0.12600247627223007	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1322	0.12581214020535358	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1319	0.12552663610503886	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1314	0.12505079593784768	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1268	0.12067306639968861	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1267	0.12057789836625037	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1228	0.11686634506215901	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1223	0.11639050489496781	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1219	0.11600983276121483	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1155	0.10991907862116747	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1092	0.10392349251455835	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1052	0.10011677117702873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	9.516803343824024E-5	0.0
9	0.0	0.0	0.0	9.516803343824024E-5	0.0
10	0.0	0.0	0.0	9.516803343824024E-5	0.0
11	0.0	0.0	0.0	9.516803343824024E-5	0.0
12	0.0	0.0	0.0	9.516803343824024E-5	1.9033606687648047E-4
13	0.0	0.0	0.0	9.516803343824024E-5	1.9033606687648047E-4
14	0.0	0.0	0.0	9.516803343824024E-5	1.9033606687648047E-4
15	0.0	0.0	0.0	9.516803343824024E-5	4.7584016719120115E-4
16	0.0	0.0	0.0	9.516803343824024E-5	4.7584016719120115E-4
17	0.0	0.0	0.0	9.516803343824024E-5	4.7584016719120115E-4
18	0.0	0.0	0.0	9.516803343824024E-5	4.7584016719120115E-4
19	0.0	0.0	0.0	9.516803343824024E-5	4.7584016719120115E-4
20	0.0	0.0	0.0	9.516803343824024E-5	6.661762340676816E-4
21	0.0	0.0	0.0	9.516803343824024E-5	6.661762340676816E-4
22	0.0	0.0	0.0	4.7584016719120115E-4	6.661762340676816E-4
23	0.0	0.0	0.0	8.565123009441621E-4	6.661762340676816E-4
24	0.0	0.0	0.0	0.0015226885350118438	6.661762340676816E-4
25	0.0	0.0	0.0	0.0019033606687648046	6.661762340676816E-4
26	0.0	0.0	0.0	0.002759872969708967	6.661762340676816E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAG	35	0.0020661366	22.857687	10
CGCGCTT	55	5.8316116E-5	20.36412	10
GTATCGA	40	0.0044826106	20.000475	9
AGTATCG	40	0.0044863163	19.997622	8
CGGCATC	290	0.0	18.758175	25
TCTAGCG	290	0.0	18.205597	28
CGAATGT	115	9.404175E-10	18.086527	23
GTCCGTA	45	0.008804984	17.793446	1
ACGTCAC	45	0.008848568	17.778202	31
AATCGGT	45	0.0088509945	17.777353	19
AACGTAT	55	0.0013643224	17.45496	31
CTAGCGG	305	0.0	17.311888	29
GGCATCG	335	0.0	17.192802	26
CATCGTT	310	0.0	17.031042	28
TAATACT	220	0.0	16.72369	4
TATAAGA	135	4.4019544E-10	16.597729	2
TAGCGGC	320	0.0	16.500393	30
ACGGGAT	60	0.0024413303	16.00038	31
CTATTCC	330	0.0	15.996573	4
ATCGTTT	335	0.0	15.761569	29
>>END_MODULE