FastQCFastQC Report
Thu 2 Feb 2017
SRR4062807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050773
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36560.34793433025020626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25770.24524802217034508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15630.14874763626396947No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14670.1396115050538984No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14540.1383743206192013No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14370.13675646405075123No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13980.13304491074665983No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13460.12809617300787135No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13290.12647831643942126No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13260.12619281233910654No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12990.12362327543627405No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12400.11800836146341788No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12270.11677117702872077No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12000.11420164012588827No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11510.10953840648741449No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11250.10706403761802025No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10750.10230563594610825No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10670.10154429167860232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGT604.720536E-621.33301532
GGTTTCG507.196097E-419.19971520
AAGACGG4200.018.6672765
ATACCGT3050.018.3612566
GATATAC2200.018.1841431
CAAGACG4050.018.172574
CGTCGTA3000.018.13392610
ACCGTCG2950.017.8988918
TAGGACG2950.017.8980394
GGTTACG450.0088505417.77751425
TACGCCT550.001364241417.4551165
CGCAAGA3750.017.0664142
CCGTCGT3100.017.0328169
GTCGTAG3200.017.00055711
TACCGTC3200.017.0005577
GTATTAT1051.1870543E-716.764051
AGGACGT3300.016.4853885
GCGCAAG4200.016.3830471
CTAGCGG2150.016.37185129
CCCCTAC805.6139783E-516.0020471
Produced by FastQC (version 0.11.5)