FastQCFastQC Report
Thu 2 Feb 2017
SRR4062806_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062806_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194558
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33080.1507365036604182No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT27000.12303160818716115No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22820.10398449254929694No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT21980.10015684251680748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA400.00448501519.999817
GTATCAA28000.019.903661
GGTATCA10600.019.4892711
CTAGCGG3700.019.45971129
TCTAGCG4050.017.77800628
TACCGTC4550.017.5810417
TAGCGGC4150.017.34922630
GTCCTAC16850.017.2024541
ACCGTCG4450.016.8975548
ATACCGT4700.016.6768846
GTAGGAC17800.016.268763
GTCCTAA9550.016.0982841
TAGGTCG805.6371457E-515.99583155
TAGGACG17000.015.7139094
TTAGGAC11900.015.5957723
TAAACGC3200.015.50019828
TCCTACA19000.015.4998822
AGGACGT16950.015.4767925
TGTAGGA19750.015.3975152
TAGGACA8050.015.3007084
Produced by FastQC (version 0.11.5)