##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062806_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2194558
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334779486347593	32.0	32.0	32.0	32.0	32.0
2	31.002595055587502	32.0	32.0	32.0	32.0	32.0
3	31.038050031031304	32.0	32.0	32.0	32.0	32.0
4	31.101352983151962	32.0	32.0	32.0	32.0	32.0
5	31.0304279950678	32.0	32.0	32.0	32.0	32.0
6	34.69996418413184	36.0	36.0	36.0	32.0	36.0
7	34.65404195286705	36.0	36.0	36.0	32.0	36.0
8	34.63829572970958	36.0	36.0	36.0	32.0	36.0
9	34.76874887790617	36.0	36.0	36.0	32.0	36.0
10	34.50657307758556	36.0	36.0	36.0	32.0	36.0
11	34.7295642220438	36.0	36.0	36.0	32.0	36.0
12	34.57311540638251	36.0	36.0	36.0	32.0	36.0
13	34.64139977161688	36.0	36.0	36.0	32.0	36.0
14	34.56141692313441	36.0	36.0	36.0	32.0	36.0
15	34.50630058535705	36.0	36.0	36.0	32.0	36.0
16	34.5181690344935	36.0	36.0	36.0	32.0	36.0
17	34.46881148732456	36.0	36.0	36.0	32.0	36.0
18	34.48913038525298	36.0	36.0	36.0	32.0	36.0
19	34.47053074013081	36.0	36.0	36.0	32.0	36.0
20	34.45926423452923	36.0	36.0	36.0	32.0	36.0
21	34.441784632714196	36.0	36.0	36.0	32.0	36.0
22	34.41932908585692	36.0	36.0	36.0	32.0	36.0
23	34.374539201060074	36.0	36.0	36.0	32.0	36.0
24	34.35894289419555	36.0	36.0	36.0	32.0	36.0
25	34.329871892198796	36.0	36.0	36.0	32.0	36.0
26	34.2964255216768	36.0	36.0	36.0	32.0	36.0
27	34.29453493596433	36.0	36.0	36.0	32.0	36.0
28	34.27269226878487	36.0	36.0	36.0	32.0	36.0
29	34.240579652030156	36.0	36.0	36.0	32.0	36.0
30	34.20982448401911	36.0	36.0	36.0	32.0	36.0
31	34.21980325878833	36.0	36.0	36.0	32.0	36.0
32	34.19007836657769	36.0	36.0	36.0	32.0	36.0
33	34.16662033995	36.0	36.0	36.0	32.0	36.0
34	34.169693396118944	36.0	36.0	36.0	32.0	36.0
35	34.14339926308624	36.0	36.0	36.0	32.0	36.0
36	34.10451033875614	36.0	36.0	36.0	32.0	36.0
37	34.100287164886964	36.0	36.0	36.0	32.0	36.0
38	33.529430527696235	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	6.0
9	5.0
10	15.0
11	20.0
12	13.0
13	13.0
14	136.0
15	348.0
16	556.0
17	670.0
18	788.0
19	973.0
20	1453.0
21	2017.0
22	3121.0
23	5028.0
24	7575.0
25	11956.0
26	17837.0
27	26356.0
28	37506.0
29	50812.0
30	69009.0
31	93545.0
32	129750.0
33	199136.0
34	464199.0
35	1071712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.150969048835684	17.269189356887363	11.368165559529892	26.21167603474706
2	16.36651204524295	18.824254073972078	38.585355311642566	26.2238785691424
3	19.51100021647241	22.552733818687265	28.39092638798692	29.5453395768534
4	12.560412669914195	14.984073748342439	35.80923130203373	36.64628227970964
5	14.477013034119098	36.323060602912314	34.145300080016476	15.054626282952116
6	35.098209423821345	35.33479605111766	16.38088988833676	13.186104636724242
7	30.787156229181456	30.09079732684212	20.283173194784553	18.838873249191863
8	27.81460635245827	34.53438332743995	18.742777569180408	18.90823275092137
9	27.260390949596136	14.150210019894702	18.660693699038617	39.92870533147054
10	16.02694495631902	26.624617005415242	31.866835448575287	25.48160258969045
11	37.81608221190563	20.794623344287874	22.318644043081783	19.070650400724713
12	24.93463209420634	23.363341660203545	29.087556083339184	22.614470162250935
13	29.990188040529365	19.512537478449467	24.94962996653543	25.54764451448574
14	22.855390313904454	20.13478177256077	24.810628328416186	32.19919958511859
15	24.725539429479344	28.592531281463206	21.718972977136602	24.962956311920845
16	25.222608724976887	25.737847835389626	24.70331369020303	24.33622974943046
17	23.62673523913006	26.596593193131696	25.46905807359629	24.307613494141957
18	24.484231776143854	24.921107614909467	27.099608329213083	23.4950522797336
19	25.72042471746263	24.696910317170982	25.240658038643822	24.34200692672257
20	25.881931020403325	24.66326352983851	25.176377090163076	24.278428359595086
21	26.282420223976665	24.24548571949372	24.50236394499698	24.969730111532634
22	25.872699482956147	25.108207353451835	25.06368489216633	23.955408271425693
23	24.267495459941532	25.057932741056028	25.85930717682417	24.815264622178272
24	25.095704595461232	24.910356695584646	25.724028051472665	24.269910657481457
25	24.67819471840101	25.059657713300254	25.775276822727722	24.486870745571018
26	23.951709091439437	26.18586320978944	26.118455744651953	23.743971954119168
27	24.762859584164058	25.418809455995444	25.255984050128166	24.562346909712332
28	24.003970038784438	25.320845392235004	25.64183888623825	25.033345682742315
29	24.381048299811102	25.2154316950848	25.450174655872104	24.95334534923199
30	23.819112743074257	25.50209752225342	26.07890474979654	24.599884984875782
31	25.200711250128734	24.890701343300314	25.03615946803886	24.872427938532095
32	24.51141764756814	25.284153827224632	24.932806540254557	25.271621984952674
33	23.803109134135354	25.344050441743494	25.97648622180459	24.876354202316563
34	24.90280495046482	25.258453838788608	25.896197373284135	23.942543837462445
35	24.826487395754576	25.127368665234723	26.15780270082184	23.888341238188858
36	23.756824748657838	25.505464356354423	25.925887103389815	24.811823791597927
37	24.825324923895806	25.140997830802604	25.10809529885707	24.925581946444524
38	24.166159320167843	25.717500355446347	25.254189709919324	24.862150614466483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	51.0
2	88.0
3	88.0
4	233.0
5	378.0
6	378.0
7	507.5
8	637.0
9	678.5
10	720.0
11	720.0
12	1091.5
13	1463.0
14	2305.5
15	3148.0
16	3148.0
17	4710.5
18	6273.0
19	6273.0
20	8255.5
21	10238.0
22	12654.5
23	15071.0
24	15071.0
25	19208.0
26	23345.0
27	23345.0
28	30710.0
29	38075.0
30	50188.5
31	62302.0
32	62302.0
33	79047.0
34	95792.0
35	95792.0
36	105919.0
37	116046.0
38	126886.0
39	137726.0
40	137726.0
41	141179.5
42	144633.0
43	155340.5
44	166048.0
45	166048.0
46	168182.0
47	170316.0
48	170316.0
49	179545.5
50	188775.0
51	191760.0
52	194745.0
53	194745.0
54	188773.5
55	182802.0
56	182802.0
57	175113.5
58	167425.0
59	152492.0
60	137559.0
61	137559.0
62	127337.5
63	117116.0
64	98360.5
65	79605.0
66	79605.0
67	66548.0
68	53491.0
69	53491.0
70	42757.5
71	32024.0
72	25298.0
73	18572.0
74	18572.0
75	14314.0
76	10056.0
77	10056.0
78	8887.5
79	7719.0
80	6025.0
81	4331.0
82	4331.0
83	4033.5
84	3736.0
85	3736.0
86	2551.0
87	1366.0
88	1149.5
89	933.0
90	933.0
91	673.5
92	414.0
93	352.5
94	291.0
95	291.0
96	266.0
97	241.0
98	241.0
99	642.5
100	1044.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12385181890840889
2	0.05668567429067722
3	0.01289553522850615
4	0.003098573835824799
5	4.556726229154117E-4
6	5.923744097900351E-4
7	0.0
8	2.73403573749247E-4
9	1.8226904916616467E-4
10	4.556726229154117E-4
11	0.0015492869179123995
12	0.0025517666883263053
13	0.013533476900587727
14	0.008931183409142068
15	0.024013947227642198
16	0.010799441163095256
17	0.02187228589993976
18	0.005149100638944152
19	0.007199627442063504
20	0.0051035333766526105
21	0.006060445884774975
22	0.007290761966646587
23	0.009250154245182857
24	0.013943582261211597
25	0.017042156097036396
26	0.02000402814598657
27	0.008338808999352033
28	0.005513638737276482
29	0.011072844736844504
30	0.0029163047866586347
31	0.0051035333766526105
32	0.006743954819148093
33	0.008429943523935115
34	0.011801920933509161
35	0.015356167392249374
36	0.015401734654540915
37	0.009022317933725151
38	0.0061060131470665165
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2194558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.059847185369826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.16066106872451	28.488679553023243
2	16.174347926927247	13.929299001129811
3	6.989868290329499	9.029479812823515
4	3.597419382474021	6.1961731548407535
5	2.026009562628441	4.3619831081439315
6	1.290865808630784	3.335069067387625
7	0.8510653505488543	2.5652720757577807
8	0.5836693367140185	2.0106169956553446
9	0.4143804221376765	1.605884188847165
>10	1.7273627567089282	12.455620886596037
>50	0.0915754585734853	2.7593272918004037
>100	0.07641345717700568	6.7567209502979
>500	0.011367831763219996	3.2059315111694304
>1k	0.004993346662348946	3.2999424025270745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3308	0.1507365036604182	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2700	0.12303160818716115	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2282	0.10398449254929694	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2198	0.10015684251680748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.556726229154117E-5
2	0.0	0.0	0.0	0.0	4.556726229154117E-5
3	0.0	0.0	0.0	0.0	4.556726229154117E-5
4	0.0	0.0	0.0	0.0	4.556726229154117E-5
5	0.0	0.0	0.0	0.0	4.556726229154117E-5
6	0.0	0.0	0.0	4.556726229154117E-5	1.367017868746235E-4
7	0.0	0.0	0.0	4.556726229154117E-5	1.8226904916616467E-4
8	0.0	0.0	0.0	4.556726229154117E-5	1.8226904916616467E-4
9	0.0	0.0	0.0	4.556726229154117E-5	1.8226904916616467E-4
10	4.556726229154117E-5	0.0	0.0	4.556726229154117E-5	1.8226904916616467E-4
11	4.556726229154117E-5	0.0	0.0	4.556726229154117E-5	1.8226904916616467E-4
12	9.113452458308233E-5	0.0	0.0	4.556726229154117E-5	7.290761966646587E-4
13	9.113452458308233E-5	0.0	0.0	1.367017868746235E-4	8.657779835392822E-4
14	9.113452458308233E-5	0.0	0.0	1.367017868746235E-4	9.569125081223645E-4
15	9.113452458308233E-5	0.0	0.0	1.367017868746235E-4	0.001093614294996988
16	9.113452458308233E-5	0.0	0.0	1.8226904916616467E-4	0.001139181557288529
17	9.113452458308233E-5	0.0	0.0	2.73403573749247E-4	0.001139181557288529
18	9.113452458308233E-5	0.0	0.0	2.73403573749247E-4	0.0012758833441631526
19	9.113452458308233E-5	0.0	0.0	2.73403573749247E-4	0.0012758833441631526
20	9.113452458308233E-5	0.0	0.0	4.101053606238705E-4	0.0012758833441631526
21	9.113452458308233E-5	0.0	0.0	6.379416720815763E-4	0.0012758833441631526
22	9.113452458308233E-5	0.0	0.0	6.835089343731175E-4	0.0013214506064546938
23	9.113452458308233E-5	0.0	0.0	8.20210721247741E-4	0.0014125851310377762
24	9.113452458308233E-5	0.0	0.0	0.001048047032705447	0.0014125851310377762
25	9.113452458308233E-5	0.0	0.0	0.001367017868746235	0.0014125851310377762
26	9.113452458308233E-5	0.0	0.0	0.0018682577539531878	0.0014125851310377762
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	40	0.004485015	19.9998	17
GTATCAA	2800	0.0	19.90366	1
GGTATCA	1060	0.0	19.489271	1
CTAGCGG	370	0.0	19.459711	29
TCTAGCG	405	0.0	17.778006	28
TACCGTC	455	0.0	17.581041	7
TAGCGGC	415	0.0	17.349226	30
GTCCTAC	1685	0.0	17.202454	1
ACCGTCG	445	0.0	16.897554	8
ATACCGT	470	0.0	16.676884	6
GTAGGAC	1780	0.0	16.26876	3
GTCCTAA	955	0.0	16.098284	1
TAGGTCG	80	5.6371457E-5	15.9958315	5
TAGGACG	1700	0.0	15.713909	4
TTAGGAC	1190	0.0	15.595772	3
TAAACGC	320	0.0	15.500198	28
TCCTACA	1900	0.0	15.499882	2
AGGACGT	1695	0.0	15.476792	5
TGTAGGA	1975	0.0	15.397515	2
TAGGACA	805	0.0	15.300708	4
>>END_MODULE