FastQCFastQC Report
Thu 2 Feb 2017
SRR4062806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194558
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32550.1483214387589665No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT27120.12357841533465964No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24130.10995380390948883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4700.021.78616328
CTAGCGG5050.020.2762329
TTATCGT400.00448366720.000844
TAGCGGC5150.019.88251930
GTATCAA25450.019.3022791
GGTATCA9450.018.9646241
ATACCGT5650.017.560266
ACCGTCG5200.017.2334588
TACGACT652.1008257E-417.2314934
TACCGTC5450.017.0301787
TAAACGC2700.016.59177828
CGCAAGA5700.016.5613442
GTAGGAC19250.016.3743423
CGTCGTA5450.016.14820310
CCGTCGT5550.016.1459269
CAAGACG5950.016.1351324
GACGGAC5700.016.0024957
TGTAGGA19800.015.9191352
CGGTCCA6750.015.88287310
AGCGGCG6550.015.87708231
Produced by FastQC (version 0.11.5)