FastQCFastQC Report
Thu 2 Feb 2017
SRR4062805_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062805_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239114
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24870.20070792517879713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT22420.1809357331125304No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19840.16011440432438018No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17700.14284399982568188No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17380.1402615094333532No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16720.1349351229991752No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15850.12791397724503153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15470.12484726990414118No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15200.12266829363561382No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14880.12008580324328513No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14200.11459801115958661No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14150.11419449703578524No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13960.11266114336534007No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13900.11217692641677843No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13230.10676983715784019No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA12770.10305750721886768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGT604.7250287E-621.3312638
CTAGCGG3900.020.51248429
TAGACCG400.004489337619.9956385
TCTAGCG4050.019.35692828
TAGACAG1301.2732926E-1118.4575125
CGCGGTC2000.017.60042210
TAGCGGC4550.017.5821330
AGCGGCG4700.017.02099831
AACCGCG2350.017.0196257
CGTCTTA1151.8406354E-816.69605315
AGAACCG2750.016.2873555
ATAGAAC3750.016.2111033
CGGTCCA5050.016.15880410
GTATTAA908.504618E-616.015251
CCCTATA703.681272E-416.0087832
ATGCGGG600.00244297515.99909226
GAACCGC2750.015.7062996
CTTGCGC4900.015.6713153
TACCGTC2500.015.3585077
CGCCGGT5250.015.2366157
Produced by FastQC (version 0.11.5)