##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062805_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1239114
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25989860497097	32.0	32.0	32.0	32.0	32.0
2	30.870700355253835	32.0	32.0	32.0	32.0	32.0
3	30.92021234527251	32.0	32.0	32.0	32.0	32.0
4	30.997214138489277	32.0	32.0	32.0	32.0	32.0
5	30.887472823323762	32.0	32.0	32.0	32.0	32.0
6	34.55421212253271	36.0	36.0	36.0	32.0	36.0
7	34.48540973631159	36.0	36.0	36.0	32.0	36.0
8	34.479428042940356	36.0	36.0	36.0	32.0	36.0
9	34.62089605960388	36.0	36.0	36.0	32.0	36.0
10	34.3241880892315	36.0	36.0	36.0	32.0	36.0
11	34.59955903976551	36.0	36.0	36.0	32.0	36.0
12	34.40803106090319	36.0	36.0	36.0	32.0	36.0
13	34.498778159233126	36.0	36.0	36.0	32.0	36.0
14	34.3919711987759	36.0	36.0	36.0	32.0	36.0
15	34.33917541081773	36.0	36.0	36.0	32.0	36.0
16	34.35291668078966	36.0	36.0	36.0	32.0	36.0
17	34.2781527769035	36.0	36.0	36.0	32.0	36.0
18	34.30299229933646	36.0	36.0	36.0	32.0	36.0
19	34.28865060034831	36.0	36.0	36.0	32.0	36.0
20	34.28553385725607	36.0	36.0	36.0	32.0	36.0
21	34.25810861631779	36.0	36.0	36.0	32.0	36.0
22	34.231685704463025	36.0	36.0	36.0	32.0	36.0
23	34.17063724564487	36.0	36.0	36.0	32.0	36.0
24	34.142948106469625	36.0	36.0	36.0	32.0	36.0
25	34.12113413293692	36.0	36.0	36.0	32.0	36.0
26	34.05993153172347	36.0	36.0	36.0	32.0	36.0
27	34.06693411582792	36.0	36.0	36.0	32.0	36.0
28	34.04419851603646	36.0	36.0	36.0	32.0	36.0
29	34.011575206155364	36.0	36.0	36.0	32.0	36.0
30	33.97421867560208	36.0	36.0	36.0	32.0	36.0
31	34.009026610949434	36.0	36.0	36.0	32.0	36.0
32	33.95565541185073	36.0	36.0	36.0	32.0	36.0
33	33.912503611451406	36.0	36.0	36.0	32.0	36.0
34	33.933609821210965	36.0	36.0	36.0	32.0	36.0
35	33.880199884756365	36.0	36.0	36.0	32.0	36.0
36	33.835972315702996	36.0	36.0	36.0	32.0	36.0
37	33.82901169706742	36.0	36.0	36.0	32.0	36.0
38	33.213073211988565	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	1.0
9	4.0
10	12.0
11	15.0
12	6.0
13	6.0
14	107.0
15	282.0
16	366.0
17	433.0
18	566.0
19	741.0
20	989.0
21	1507.0
22	2300.0
23	3358.0
24	5406.0
25	7956.0
26	11639.0
27	16954.0
28	24249.0
29	33084.0
30	44427.0
31	59640.0
32	81730.0
33	122173.0
34	266275.0
35	554886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.600454414706746	16.415569682846975	10.990226528640084	25.993749373806192
2	17.149326053271384	19.04395087088629	36.82479161468641	26.981931461155913
3	19.46628376889569	23.213580925492323	27.826514379247975	29.493620926364013
4	12.73788761527678	14.525015676915912	35.06125094122024	37.67584576658706
5	14.949229852943313	36.075938129986426	33.438811925295006	15.536020091775251
6	35.77955737478684	34.413735052743625	16.280030699527966	13.526676872941563
7	31.205709245242364	29.650161042616773	20.448417537383595	18.695712174757265
8	29.04033617650073	32.35787592879088	19.015729825657264	19.58605806905112
9	27.522312367495726	14.001005559631057	17.98402241607088	40.49265965680234
10	16.006017226894485	26.18413715016193	31.107190731404582	26.702654891539
11	38.703676652766674	20.56680351451752	21.25731081463882	19.472209018076992
12	25.036982994682354	23.08150462882632	28.137985913776557	23.743526462714765
13	30.03015510182997	18.539011145119005	24.938495215218317	26.492338537832705
14	24.0041646993305	19.08045698696917	24.266072632033477	32.649305681666846
15	25.878969235774036	26.246096048704842	21.985067916038712	25.88986679948241
16	26.850184548306622	24.992756085493525	22.942425609638967	25.21463375656089
17	24.806614537549834	25.19253790016039	24.70167825405397	25.299169308235804
18	25.911126571071385	23.916441963676803	25.616705796328294	24.555725668923518
19	26.138063119596	24.791830271353085	24.503374863701996	24.566731745348918
20	26.02076928850619	23.613262192292265	24.693533143778133	25.672435375423415
21	27.354557949626845	23.876442354642695	23.568460242786106	25.200539452944355
22	26.11291315818996	23.757831775519904	24.484452669563034	25.6448023967271
23	24.7103081625422	23.44282314047986	24.97156956195737	26.87529913502057
24	25.204068372287868	24.82494876598465	24.381256946557613	25.58972591516987
25	25.649147022144735	23.75181108972108	24.419583580529423	26.179458307604758
26	25.018242577853833	24.750496424131864	25.04108615985664	25.19017483815766
27	26.060978306663934	24.134179393348496	24.176794474244513	25.62804782574306
28	25.217466397426396	23.844550870753373	25.08833437446027	25.84964835735996
29	24.748579453735392	24.449619035319945	25.3219635823594	25.479837928585265
30	25.107701048682973	24.513630296074986	25.363455730793383	25.015212924448665
31	25.512010950448577	24.40260008587321	24.07952634144609	26.005862622232122
32	25.18252122217792	24.427255426404766	24.045183586568246	26.345039764849066
33	24.777825396710096	23.88694781207148	24.78194160950377	26.553285181714653
34	25.750532909642253	24.279371372441087	24.8918239836378	25.07827173427886
35	26.40521039916636	23.933192725523384	24.88193221928684	24.779664656023414
36	25.049902857922348	24.916236650531477	24.293510328055294	25.740350163490884
37	26.37052832421168	24.535086154643327	24.412163749109155	24.682221772035838
38	25.19365830587924	24.33169926991747	24.627412557161282	25.84722986704201
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	81.5
2	108.0
3	108.0
4	310.5
5	513.0
6	513.0
7	618.5
8	724.0
9	699.0
10	674.0
11	674.0
12	902.5
13	1131.0
14	1523.5
15	1916.0
16	1916.0
17	2832.0
18	3748.0
19	3748.0
20	4573.0
21	5398.0
22	5987.5
23	6577.0
24	6577.0
25	8006.5
26	9436.0
27	9436.0
28	12276.5
29	15117.0
30	18442.0
31	21767.0
32	21767.0
33	28733.5
34	35700.0
35	35700.0
36	40657.0
37	45614.0
38	53292.0
39	60970.0
40	60970.0
41	65730.5
42	70491.0
43	81784.0
44	93077.0
45	93077.0
46	99519.0
47	105961.0
48	105961.0
49	112389.0
50	118817.0
51	119422.5
52	120028.0
53	120028.0
54	115959.0
55	111890.0
56	111890.0
57	109792.5
58	107695.0
59	97802.5
60	87910.0
61	87910.0
62	82837.5
63	77765.0
64	64636.0
65	51507.0
66	51507.0
67	42587.5
68	33668.0
69	33668.0
70	26627.0
71	19586.0
72	15496.5
73	11407.0
74	11407.0
75	8615.0
76	5823.0
77	5823.0
78	5479.5
79	5136.0
80	4066.5
81	2997.0
82	2997.0
83	2940.5
84	2884.0
85	2884.0
86	1924.0
87	964.0
88	811.5
89	659.0
90	659.0
91	494.5
92	330.0
93	283.5
94	237.0
95	237.0
96	220.5
97	204.0
98	204.0
99	417.0
100	630.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11927877499568239
2	0.05350597281606051
3	0.011379098291198387
4	0.0025017875675684403
5	0.0
6	5.649197733219059E-4
7	8.070282476027226E-5
8	2.4210847428081676E-4
9	2.4210847428081676E-4
10	4.035141238013613E-4
11	0.0020175706190068065
12	0.0025017875675684403
13	0.015010725405410642
14	0.010410664394075122
15	0.02461436155188304
16	0.011540503940718932
17	0.021547654210992695
18	0.00572990055797933
19	0.008150985300787499
20	0.005891306207499875
21	0.006536928805582053
22	0.007343957053184776
23	0.010087853095034034
24	0.015414239529212002
25	0.017673918622499625
26	0.020498517489109153
27	0.009361527672191583
28	0.005810603382739602
29	0.012428235013081927
30	0.002905301691369801
31	0.006294820331301236
32	0.006456225980821781
33	0.008958013548390221
34	0.012993154786403832
35	0.016705484725376356
36	0.016705484725376356
37	0.009765041795992944
38	0.006133414681780691
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1239114.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.33454702065988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14380000494829	39.601420287064
2	13.073971783308872	13.422928385140995
3	4.31299170584945	6.642164265709341
4	1.8561686558766333	3.8114230857349627
5	0.9814478512576343	2.519109043435532
6	0.5806689338878757	1.7885026014062155
7	0.3764627946123829	1.3527882922090873
8	0.28238985194477845	1.1597084106253128
9	0.20835570709752463	0.9626261258718642
>10	1.0014476096603004	9.209262558678889
>50	0.07878235766685446	2.8535508285826094
>100	0.08836454329607418	9.76941503707683
>500	0.010887769176442606	3.800971162259501
>1k	0.004260431416868846	3.1061299162049876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2487	0.20070792517879713	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2242	0.1809357331125304	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1984	0.16011440432438018	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1770	0.14284399982568188	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1738	0.1402615094333532	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1672	0.1349351229991752	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1585	0.12791397724503153	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1547	0.12484726990414118	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1520	0.12266829363561382	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1488	0.12008580324328513	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1420	0.11459801115958661	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1415	0.11419449703578524	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1396	0.11266114336534007	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1390	0.11217692641677843	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1323	0.10676983715784019	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1277	0.10305750721886768	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.070282476027226E-5	0.0
8	0.0	0.0	0.0	8.070282476027226E-5	0.0
9	0.0	0.0	0.0	8.070282476027226E-5	0.0
10	0.0	0.0	0.0	8.070282476027226E-5	0.0
11	0.0	0.0	0.0	8.070282476027226E-5	0.0
12	0.0	0.0	0.0	8.070282476027226E-5	8.070282476027226E-5
13	0.0	0.0	0.0	8.070282476027226E-5	8.070282476027226E-5
14	0.0	0.0	0.0	1.6140564952054452E-4	8.070282476027226E-5
15	0.0	0.0	0.0	1.6140564952054452E-4	2.421084742808168E-4
16	0.0	0.0	0.0	2.421084742808168E-4	2.421084742808168E-4
17	0.0	0.0	0.0	2.421084742808168E-4	2.421084742808168E-4
18	0.0	0.0	0.0	4.035141238013613E-4	3.2281129904108903E-4
19	0.0	0.0	0.0	4.035141238013613E-4	3.2281129904108903E-4
20	0.0	0.0	0.0	4.035141238013613E-4	3.2281129904108903E-4
21	0.0	0.0	0.0	4.842169485616336E-4	3.2281129904108903E-4
22	0.0	0.0	0.0	5.649197733219059E-4	4.035141238013613E-4
23	0.0	0.0	0.0	8.877310723629948E-4	4.035141238013613E-4
24	0.0	0.0	0.0	0.0011298395466438117	4.035141238013613E-4
25	0.0	0.0	0.0	0.0014526508456849006	4.035141238013613E-4
26	0.0	0.0	0.0	0.0020982734437670785	4.035141238013613E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTGT	60	4.7250287E-6	21.331263	8
CTAGCGG	390	0.0	20.512484	29
TAGACCG	40	0.0044893376	19.995638	5
TCTAGCG	405	0.0	19.356928	28
TAGACAG	130	1.2732926E-11	18.457512	5
CGCGGTC	200	0.0	17.600422	10
TAGCGGC	455	0.0	17.58213	30
AGCGGCG	470	0.0	17.020998	31
AACCGCG	235	0.0	17.019625	7
CGTCTTA	115	1.8406354E-8	16.696053	15
AGAACCG	275	0.0	16.287355	5
ATAGAAC	375	0.0	16.211103	3
CGGTCCA	505	0.0	16.158804	10
GTATTAA	90	8.504618E-6	16.01525	1
CCCTATA	70	3.681272E-4	16.008783	2
ATGCGGG	60	0.002442975	15.999092	26
GAACCGC	275	0.0	15.706299	6
CTTGCGC	490	0.0	15.671315	3
TACCGTC	250	0.0	15.358507	7
CGCCGGT	525	0.0	15.236615	7
>>END_MODULE