FastQCFastQC Report
Thu 2 Feb 2017
SRR4062805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239114
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA25650.20700274551009834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24470.19747981218838623No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC23700.19126569468184526No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20310.16390743708811295No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17680.14268259417616136No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16990.13711409926770257No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16760.1352579342982163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16360.1320298213078054No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15700.12670343487362745No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15520.12525078402794254No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15330.12371743035749737No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14900.12024720889280567No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14620.11798752979951803No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13980.11282254901486062No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13270.10709264845688128No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12740.10281539874458685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3900.020.51186428
CTAGCGG3950.019.84717629
CGTTTAT2400.018.66579631
TAGCGGC4250.018.446230
ATCGTTT2450.018.28486329
GTATAGA802.795632E-618.0006121
GGTTCGT802.7977876E-617.99915926
AAATTAG2250.017.0672471
AGCGGCG4650.016.85942831
CGCCGGT5050.016.7946857
CGTCTTA1051.1899101E-716.76112415
ACCGTCG2450.016.3290638
AATTAGA2600.015.9998992
TGGTTCG908.585685E-615.99925425
CATCGTT2900.015.99925428
ACCCGTT1407.4396667E-1015.99925430
GCCGGTC5800.015.7265788
TAGAGTG2850.015.7211035
TAATACT1950.015.5915334
CGGGTAA1451.2223609E-915.44755624
Produced by FastQC (version 0.11.5)