FastQCFastQC Report
Thu 2 Feb 2017
SRR4062804_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062804_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014038
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31310.30876554922004895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19920.19644234239742497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11750.1158733696370353No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11680.11518306020089977No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10540.10394087795526401No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10530.10384226232153036No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10240.10098240894325458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGT507.2075316E-419.1950916
TAGGACG1551.0913936E-1116.5119064
ATCACGA2550.016.3127725
TCACGAA2950.016.27023526
ACGAACG1601.8189894E-1116.00064315
CAAGACG3050.015.7336794
TCGTTTA1950.015.59036730
ACCGTCG1950.015.5880628
TACCGTC1950.015.5880627
TAACGAA1652.910383E-1115.51577213
CGAACGA1652.910383E-1115.51500816
CACGAAT2700.015.40574527
ATACCGT2200.015.268826
CTATAAC951.4341926E-515.1555123
AACGAAC1704.7293724E-1115.05942614
ATAACGA1704.7293724E-1115.05942612
GTAATAC859.443088E-515.0564573
TTCCGAA1603.0377123E-1014.99986320
CTAGCGG2350.014.97932429
TCTAGCG2350.014.97784728
Produced by FastQC (version 0.11.5)