##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062804_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1014038
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25092945234794	32.0	32.0	32.0	32.0	32.0
2	30.93974979241409	32.0	32.0	32.0	32.0	32.0
3	30.999784031762122	32.0	32.0	32.0	32.0	32.0
4	31.04866089830953	32.0	32.0	32.0	32.0	32.0
5	31.003286859072343	32.0	32.0	32.0	32.0	32.0
6	34.636297653539614	36.0	36.0	36.0	32.0	36.0
7	34.57315011863461	36.0	36.0	36.0	32.0	36.0
8	34.56047209276181	36.0	36.0	36.0	32.0	36.0
9	34.65148840575994	36.0	36.0	36.0	32.0	36.0
10	34.4449241547161	36.0	36.0	36.0	32.0	36.0
11	34.649973669625794	36.0	36.0	36.0	32.0	36.0
12	34.510679086977014	36.0	36.0	36.0	32.0	36.0
13	34.568163126036694	36.0	36.0	36.0	32.0	36.0
14	34.479689124076216	36.0	36.0	36.0	32.0	36.0
15	34.44755521982411	36.0	36.0	36.0	32.0	36.0
16	34.45592670097176	36.0	36.0	36.0	32.0	36.0
17	34.392197333827724	36.0	36.0	36.0	32.0	36.0
18	34.41408901836026	36.0	36.0	36.0	32.0	36.0
19	34.40062108126126	36.0	36.0	36.0	32.0	36.0
20	34.36398340101653	36.0	36.0	36.0	32.0	36.0
21	34.344891414325694	36.0	36.0	36.0	32.0	36.0
22	34.33084558961301	36.0	36.0	36.0	32.0	36.0
23	34.28483449338191	36.0	36.0	36.0	32.0	36.0
24	34.276305227220284	36.0	36.0	36.0	32.0	36.0
25	34.25022336441041	36.0	36.0	36.0	32.0	36.0
26	34.20308410532939	36.0	36.0	36.0	32.0	36.0
27	34.21225042848493	36.0	36.0	36.0	32.0	36.0
28	34.191754155169725	36.0	36.0	36.0	32.0	36.0
29	34.15724657261365	36.0	36.0	36.0	32.0	36.0
30	34.12783544600892	36.0	36.0	36.0	32.0	36.0
31	34.15470426157599	36.0	36.0	36.0	32.0	36.0
32	34.10238176478593	36.0	36.0	36.0	32.0	36.0
33	34.05354533064836	36.0	36.0	36.0	32.0	36.0
34	34.05550778175966	36.0	36.0	36.0	32.0	36.0
35	34.01808906569576	36.0	36.0	36.0	32.0	36.0
36	33.97548513961015	36.0	36.0	36.0	32.0	36.0
37	33.967974572944996	36.0	36.0	36.0	32.0	36.0
38	33.37611706859112	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	6.0
9	5.0
10	8.0
11	5.0
12	4.0
13	3.0
14	129.0
15	300.0
16	394.0
17	449.0
18	574.0
19	763.0
20	974.0
21	1285.0
22	1925.0
23	2762.0
24	3997.0
25	6012.0
26	8667.0
27	12506.0
28	17874.0
29	23998.0
30	32956.0
31	44940.0
32	61973.0
33	93465.0
34	214428.0
35	483633.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77385101790542	17.045299703979	11.44351212422218	25.737337153893396
2	16.502129851369233	19.841807842707738	37.943620362503964	25.71244194341907
3	19.21007262929079	24.25792669215201	28.297906335436075	28.234094343121125
4	12.629176828396368	15.617982735989633	35.96941668630802	35.783423749305975
5	14.664878431217856	36.512546411119935	33.52172262298639	15.300852534675824
6	33.78364652107669	35.341186448199515	17.231653739762436	13.643513290961357
7	29.949104421239063	30.651544273039345	20.916593773205513	18.48275753251607
8	27.87166060175734	32.91283295366015	19.764109543110166	19.451396901472343
9	27.716822118390503	14.402574272657526	18.47009074646412	39.41051286248785
10	15.95074297751147	26.742851282213113	31.696363414028017	25.610042326247402
11	37.25102685857704	21.294359550894217	22.26939443696592	19.185219153562816
12	25.27339554855144	23.704274230848505	28.64773122867988	22.374598991920173
13	29.85973030977292	19.793589925673434	25.0	25.34667976455365
14	23.813327889272536	19.83886763535444	25.29338260602851	31.054421869344512
15	25.2764118978689	26.97340192050661	22.83725999832313	24.912926183301355
16	25.41859192157288	25.818913441196923	24.16486263912381	24.597631998106387
17	23.985386167755273	25.725118707796348	25.399720269787757	24.889774854660626
18	24.795461090269313	24.819327250421107	26.526448044750033	23.858763614559543
19	25.454154552591195	25.080081106776124	25.745089607997063	23.720674732635615
20	25.78028844636602	24.2291791586455	25.500007889670844	24.490524505317637
21	26.88649240373378	24.25953046642702	24.593854938682526	24.260122191156675
22	25.7782805340613	24.415710191465987	25.172343056867785	24.63366621760493
23	24.107816498922524	24.347277219178558	25.78039242759716	25.76451385430176
24	24.517430305899527	25.156845629971546	25.574443112945623	24.751280951183308
25	24.82532311771111	24.440955071251597	25.54020625877823	25.19351555225906
26	24.304611005299563	25.379614679387725	26.02733916124266	24.28843515407005
27	25.115487790445385	24.95325259379068	25.15247149788946	24.778788117874473
28	24.347156324489365	24.86382961517258	25.87054863622512	24.91846542411293
29	24.196515133732508	25.152698369803744	25.914784792199043	24.736001704264705
30	24.330377394960966	25.346349718541543	25.915578242294902	24.40769464420259
31	24.845856163978272	24.999704138510516	24.907888456273643	25.24655124123757
32	24.434972390892497	25.214082765190927	24.965556417946363	25.385388425970213
33	24.108915431658616	24.84691225796653	25.651775561387467	25.392396748987387
34	24.76159573303456	25.12621692445921	25.76413141354079	24.34805592896544
35	25.36056708377347	24.911306859034866	25.669871159466172	24.05825489772549
36	24.382892306114215	25.616861117111146	25.198765538035456	24.801481038739183
37	25.07325290225484	25.44102142801491	25.14554422147772	24.340181448252533
38	24.255487014107704	25.364675019847432	25.574737298875228	24.805100667169633
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	442.0
1	363.0
2	284.0
3	284.0
4	584.0
5	884.0
6	884.0
7	996.5
8	1109.0
9	1055.5
10	1002.0
11	1002.0
12	1330.5
13	1659.0
14	2074.5
15	2490.0
16	2490.0
17	3676.0
18	4862.0
19	4862.0
20	5846.5
21	6831.0
22	7092.5
23	7354.0
24	7354.0
25	8768.5
26	10183.0
27	10183.0
28	13084.0
29	15985.0
30	20003.5
31	24022.0
32	24022.0
33	29472.0
34	34922.0
35	34922.0
36	39617.0
37	44312.0
38	50306.0
39	56300.0
40	56300.0
41	60404.5
42	64509.0
43	72301.5
44	80094.0
45	80094.0
46	84900.5
47	89707.0
48	89707.0
49	93263.0
50	96819.0
51	95805.0
52	94791.0
53	94791.0
54	90091.5
55	85392.0
56	85392.0
57	82424.0
58	79456.0
59	70841.0
60	62226.0
61	62226.0
62	57919.0
63	53612.0
64	44180.0
65	34748.0
66	34748.0
67	28866.0
68	22984.0
69	22984.0
70	18507.5
71	14031.0
72	11007.5
73	7984.0
74	7984.0
75	6288.5
76	4593.0
77	4593.0
78	4188.5
79	3784.0
80	2977.5
81	2171.0
82	2171.0
83	2186.0
84	2201.0
85	2201.0
86	1492.5
87	784.0
88	663.0
89	542.0
90	542.0
91	387.0
92	232.0
93	202.5
94	173.0
95	173.0
96	146.0
97	119.0
98	119.0
99	282.0
100	445.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12543908610919907
2	0.05788737700165083
3	0.012031107315504943
4	0.0020709283084065886
5	2.958469012009412E-4
6	2.958469012009412E-4
7	9.861563373364705E-5
8	7.889250698691764E-4
9	3.944625349345882E-4
10	9.861563373364706E-4
11	0.0022681595758738825
12	0.003057084645743059
13	0.013214494920308706
14	0.008776791402294589
15	0.023963598997276236
16	0.010157410274565648
17	0.020117589281664
18	0.004930781686682353
19	0.006508631826420705
20	0.005325244221616942
21	0.005226628587883295
22	0.007100325628822588
23	0.009171253937229177
24	0.01410203562391153
25	0.01637019519978541
26	0.017849429705790117
27	0.007692019431224471
28	0.005029397320416
29	0.010946335344434824
30	0.0033529315469440005
31	0.004930781686682353
32	0.006015553657752471
33	0.007593403797490824
34	0.010749104076967528
35	0.014693729426313413
36	0.015285423228715296
37	0.00838232886736
38	0.005226628587883295
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1014038.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.85834864486613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.53058398999555	53.341251549424086
2	11.007877242965517	14.058897256423593
3	2.6996558264225694	5.1718668895450985
4	1.011829731223301	2.5845510298279497
5	0.4858386146475349	1.5512425819650526
6	0.27599316155609993	1.0574680520548976
7	0.1805303734793907	0.8069860071444377
8	0.11269066802525722	0.5756991974223794
9	0.08625565559338497	0.4957329354726487
>10	0.48127784512803684	5.818984326477273
>50	0.05443792383281221	2.4510100151736545
>100	0.06651683067396669	8.581351666818332
>500	0.005271729512567136	2.356606601148471
>1k	0.0012404069441334438	1.1483518911020534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3131	0.30876554922004895	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1992	0.19644234239742497	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1175	0.1158733696370353	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1168	0.11518306020089977	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1054	0.10394087795526401	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1053	0.10384226232153036	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1024	0.10098240894325458	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	9.861563373364707E-5	0.0
20	0.0	0.0	0.0	2.958469012009412E-4	9.861563373364707E-5
21	0.0	0.0	0.0	2.958469012009412E-4	1.9723126746729413E-4
22	0.0	0.0	0.0	5.916938024018824E-4	1.9723126746729413E-4
23	0.0	0.0	0.0	0.0017750814072056472	1.9723126746729413E-4
24	0.0	0.0	0.0	0.0027612377445421178	1.9723126746729413E-4
25	0.0	0.0	0.0	0.0036487784481449414	1.9723126746729413E-4
26	0.0	0.0	0.0	0.004733550419215059	1.9723126746729413E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCCGT	50	7.2075316E-4	19.195091	6
TAGGACG	155	1.0913936E-11	16.511906	4
ATCACGA	255	0.0	16.31277	25
TCACGAA	295	0.0	16.270235	26
ACGAACG	160	1.8189894E-11	16.000643	15
CAAGACG	305	0.0	15.733679	4
TCGTTTA	195	0.0	15.590367	30
ACCGTCG	195	0.0	15.588062	8
TACCGTC	195	0.0	15.588062	7
TAACGAA	165	2.910383E-11	15.515772	13
CGAACGA	165	2.910383E-11	15.515008	16
CACGAAT	270	0.0	15.405745	27
ATACCGT	220	0.0	15.26882	6
CTATAAC	95	1.4341926E-5	15.155512	3
AACGAAC	170	4.7293724E-11	15.059426	14
ATAACGA	170	4.7293724E-11	15.059426	12
GTAATAC	85	9.443088E-5	15.056457	3
TTCCGAA	160	3.0377123E-10	14.999863	20
CTAGCGG	235	0.0	14.979324	29
TCTAGCG	235	0.0	14.977847	28
>>END_MODULE