FastQCFastQC Report
Thu 2 Feb 2017
SRR4062804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014038
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35230.3474228776436386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24360.24022768377516424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15510.1529528479208866No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12630.12455154540559624No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11290.11133705048528753No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.11025227851421743No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10900.10749104076967529No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10790.10640626879860518No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10180.10039071514085271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG507.19156E-419.201425
TGTACTG1550.018.582025
GTAGGAC1850.018.162613
AAGACGG2300.018.0882975
ACCGTCC550.00136381917.4558378
GTCTATA753.231033E-517.067931
TCGTTTA2250.017.06624630
CGTCGTA2550.016.94159310
CATCGTT2300.016.69524228
CGGCATC2400.016.66625425
ACCGTCG2500.016.0011838
TAATAGT1304.796675E-916.0003954
GTATATC1203.1075615E-816.0003953
ATCGTTT2400.015.99960429
CCGTCGT2550.015.6874349
CGGACCA2250.015.6456019
GGACGTG1551.891749E-1015.4850176
ATAACGA1551.891749E-1015.4834912
CGAACGA1451.2205419E-915.44789516
ACGAACG1451.2205419E-915.44789515
Produced by FastQC (version 0.11.5)