##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062804_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1014038
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.184992081164612	32.0	32.0	32.0	32.0	32.0
2	31.27216238444713	32.0	32.0	32.0	32.0	32.0
3	31.360841506925777	32.0	32.0	32.0	32.0	32.0
4	31.46044329699676	32.0	32.0	32.0	32.0	32.0
5	31.38188904163355	32.0	32.0	32.0	32.0	32.0
6	34.90497890611594	36.0	36.0	36.0	36.0	36.0
7	34.93170177054509	36.0	36.0	36.0	36.0	36.0
8	34.882574420287995	36.0	36.0	36.0	32.0	36.0
9	34.97688745392185	36.0	36.0	36.0	36.0	36.0
10	34.82218122003317	36.0	36.0	36.0	32.0	36.0
11	34.998849155554325	36.0	36.0	36.0	36.0	36.0
12	34.89895053242581	36.0	36.0	36.0	32.0	36.0
13	34.9531201000357	36.0	36.0	36.0	36.0	36.0
14	34.89683226861321	36.0	36.0	36.0	32.0	36.0
15	34.86733830487615	36.0	36.0	36.0	32.0	36.0
16	34.88191073707297	36.0	36.0	36.0	32.0	36.0
17	34.84421885570363	36.0	36.0	36.0	32.0	36.0
18	34.84445750553727	36.0	36.0	36.0	32.0	36.0
19	34.82521266461415	36.0	36.0	36.0	32.0	36.0
20	34.819570864208245	36.0	36.0	36.0	32.0	36.0
21	34.80707231878884	36.0	36.0	36.0	32.0	36.0
22	34.78701685735643	36.0	36.0	36.0	32.0	36.0
23	34.73555724736154	36.0	36.0	36.0	32.0	36.0
24	34.71549685514744	36.0	36.0	36.0	32.0	36.0
25	34.69316830335747	36.0	36.0	36.0	32.0	36.0
26	34.6295700950063	36.0	36.0	36.0	32.0	36.0
27	34.61304605941789	36.0	36.0	36.0	32.0	36.0
28	34.58836552476337	36.0	36.0	36.0	32.0	36.0
29	34.55300984775718	36.0	36.0	36.0	32.0	36.0
30	34.52259481400105	36.0	36.0	36.0	32.0	36.0
31	34.502841116407865	36.0	36.0	36.0	32.0	36.0
32	34.47957374378475	36.0	36.0	36.0	32.0	36.0
33	34.437091114928634	36.0	36.0	36.0	32.0	36.0
34	34.42450677390788	36.0	36.0	36.0	32.0	36.0
35	34.398274029178395	36.0	36.0	36.0	32.0	36.0
36	34.36491433259898	36.0	36.0	36.0	32.0	36.0
37	34.34895930921721	36.0	36.0	36.0	32.0	36.0
38	33.92290032523436	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	3.0
21	21.0
22	90.0
23	212.0
24	658.0
25	1619.0
26	3530.0
27	6942.0
28	12104.0
29	19345.0
30	28914.0
31	41967.0
32	59775.0
33	95743.0
34	230410.0
35	512701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.14133719416457	18.26106767237638	12.15718027820077	26.440414855258275
2	15.550403436557605	20.991422412177847	37.105315579889506	26.352858571375037
3	18.181074082036375	25.03584678286218	28.55701660095578	28.22606253414566
4	12.186426938635437	16.527585751224315	35.963839619422544	35.322147690717706
5	14.113869499959566	36.963703529848	33.19323338967573	15.729193580516707
6	33.757742567578894	35.80313144392167	16.83686098239019	13.602265006109244
7	29.568268488303634	30.90284044010402	21.01963151100605	18.50925956058629
8	27.8536862939627	33.120679912387715	19.53683039566554	19.48880339798405
9	27.633479981577747	14.282473907559337	18.307263082216707	39.776783028646214
10	15.725814006924866	26.964272154488985	31.706946659211244	25.602967179374907
11	37.3048410177311	21.26716378314781	22.21323673717752	19.214758461943568
12	25.017528946182438	23.868557064485813	28.548465194070825	22.565448795260924
13	29.83789530154748	19.74200127017187	24.95453810318371	25.46556532509694
14	23.734317648845508	19.844621207489265	25.26295858735077	31.15810255631446
15	25.09097292211929	26.964176884889916	22.781592011344742	25.163258181646054
16	25.501213958451263	25.887392780152226	24.03292578779099	24.578467473605524
17	24.022768377516424	25.846368676519027	25.29629067155274	24.834572274411805
18	24.78536307317872	24.823625939067373	26.521294073792106	23.869716913961806
19	25.415418357102986	25.13534995729943	25.7964691658498	23.65276251974778
20	25.84350882314075	24.040321960321016	25.624089038083387	24.49208017845485
21	26.743330611536475	24.345683979661473	24.665889573940174	24.24509583486188
22	25.629019819770065	24.499772197886074	25.16966819783874	24.701539784505115
23	24.128310899898132	24.311538928066728	25.71513662716449	25.84501354487065
24	24.5909916590897	25.09767878521318	25.528234642094283	24.783094913602845
25	24.84492691595384	24.47561136762133	25.671720389176738	25.00774132724809
26	24.42196446287023	25.25211086764007	25.957804342638045	24.368120326851656
27	25.323040480771414	24.782133196323212	25.115947445704645	24.778878877200732
28	24.501941741828215	24.734378790538422	25.89725434352559	24.866425124107774
29	24.388730994301987	24.933187908145456	25.93620751885038	24.74187357870218
30	24.428768941597852	25.098664941550513	26.076438950019625	24.396127166832013
31	24.903504602391628	24.928651588993706	24.953404113060852	25.214439695553814
32	24.592076431060768	25.10961127689495	25.086337987333806	25.211974304710473
33	24.224733195402933	24.619886039773657	25.761164768973156	25.394215995850256
34	24.83112072723113	24.949360872077772	25.88492738930888	24.334591011382216
35	25.497466564369383	24.685761283107734	25.744893189407104	24.071878963115783
36	24.52127040604001	25.3468804916581	25.33682169701727	24.795027405284614
37	25.255562414820748	25.289288961557656	25.156650934185897	24.298497689435703
38	24.473268726880775	25.02719328781889	25.682494820208728	24.817043165091608
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	206.0
1	174.0
2	142.0
3	142.0
4	487.0
5	832.0
6	832.0
7	961.5
8	1091.0
9	1089.5
10	1088.0
11	1088.0
12	1390.0
13	1692.0
14	2173.5
15	2655.0
16	2655.0
17	3967.0
18	5279.0
19	5279.0
20	6096.0
21	6913.0
22	7245.0
23	7577.0
24	7577.0
25	8871.5
26	10166.0
27	10166.0
28	13060.0
29	15954.0
30	19728.0
31	23502.0
32	23502.0
33	29799.0
34	36096.0
35	36096.0
36	40011.0
37	43926.0
38	50015.0
39	56104.0
40	56104.0
41	60252.5
42	64401.0
43	72263.0
44	80125.0
45	80125.0
46	84798.0
47	89471.0
48	89471.0
49	93335.0
50	97199.0
51	96262.0
52	95325.0
53	95325.0
54	90471.0
55	85617.0
56	85617.0
57	82548.0
58	79479.0
59	70816.0
60	62153.0
61	62153.0
62	57840.5
63	53528.0
64	44185.5
65	34843.0
66	34843.0
67	29072.5
68	23302.0
69	23302.0
70	18837.5
71	14373.0
72	11207.5
73	8042.0
74	8042.0
75	6178.5
76	4315.0
77	4315.0
78	4017.5
79	3720.0
80	2877.5
81	2035.0
82	2035.0
83	1975.0
84	1915.0
85	1915.0
86	1219.5
87	524.0
88	424.5
89	325.0
90	325.0
91	199.5
92	74.0
93	53.5
94	33.0
95	33.0
96	19.0
97	5.0
98	5.0
99	8.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009269869570962824
2	0.0
3	0.0
4	0.0
5	0.0
6	9.861563373364705E-5
7	4.930781686682353E-4
8	0.0024653908433411764
9	0.004634934785481412
10	0.0010847719710701175
11	0.0056210911228178825
12	9.861563373364705E-5
13	1.972312674672941E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.972312674672941E-4
22	0.0
23	9.861563373364705E-5
24	0.0
25	0.0
26	0.0
27	9.861563373364705E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.861563373364705E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1014038.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.455453961447404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.61721383643112	51.5989559518428
2	11.614936903422404	14.508323140736287
3	2.8873537906422833	5.409929752256092
4	1.047983078194359	2.618090355701749
5	0.5021100258979342	1.5679754803024792
6	0.2860406441075912	1.0718878967498653
7	0.18597712455185655	0.8130700018231592
8	0.13069513606789135	0.6530099242938633
9	0.09504204773787842	0.5342304813205276
>10	0.49886981608718217	5.869314107991663
>50	0.058088520971224016	2.5901997441763633
>100	0.06854859757436563	8.92607476012392
>500	0.005553705355268746	2.3305964430868182
>1k	0.0015867729586482129	1.5083419595943943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3523	0.3474228776436386	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2436	0.24022768377516424	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1551	0.1529528479208866	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1263	0.12455154540559624	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1129	0.11133705048528753	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1118	0.11025227851421743	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1090	0.10749104076967529	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1079	0.10640626879860518	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1018	0.10039071514085271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	9.861563373364707E-5
16	0.0	0.0	0.0	0.0	9.861563373364707E-5
17	0.0	0.0	0.0	0.0	9.861563373364707E-5
18	0.0	0.0	0.0	0.0	9.861563373364707E-5
19	0.0	0.0	0.0	9.861563373364707E-5	9.861563373364707E-5
20	0.0	0.0	0.0	2.958469012009412E-4	1.9723126746729413E-4
21	0.0	0.0	0.0	3.9446253493458826E-4	1.9723126746729413E-4
22	0.0	0.0	0.0	5.916938024018824E-4	1.9723126746729413E-4
23	0.0	0.0	0.0	0.0018736970409392942	1.9723126746729413E-4
24	0.0	0.0	0.0	0.0029584690120094117	1.9723126746729413E-4
25	0.0	0.0	0.0	0.0038460097156122353	1.9723126746729413E-4
26	0.0	0.0	0.0	0.005226628587883295	1.9723126746729413E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	50	7.19156E-4	19.20142	5
TGTACTG	155	0.0	18.58202	5
GTAGGAC	185	0.0	18.16261	3
AAGACGG	230	0.0	18.088297	5
ACCGTCC	55	0.001363819	17.455837	8
GTCTATA	75	3.231033E-5	17.06793	1
TCGTTTA	225	0.0	17.066246	30
CGTCGTA	255	0.0	16.941593	10
CATCGTT	230	0.0	16.695242	28
CGGCATC	240	0.0	16.666254	25
ACCGTCG	250	0.0	16.001183	8
TAATAGT	130	4.796675E-9	16.000395	4
GTATATC	120	3.1075615E-8	16.000395	3
ATCGTTT	240	0.0	15.999604	29
CCGTCGT	255	0.0	15.687434	9
CGGACCA	225	0.0	15.645601	9
GGACGTG	155	1.891749E-10	15.485017	6
ATAACGA	155	1.891749E-10	15.48349	12
CGAACGA	145	1.2205419E-9	15.447895	16
ACGAACG	145	1.2205419E-9	15.447895	15
>>END_MODULE