FastQCFastQC Report
Thu 2 Feb 2017
SRR4062803_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062803_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824559
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT58210.31903599719165016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT36390.1994454550387244No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT34770.19056659718869054No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG32270.176864656062095No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA32070.17576850077196735No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC31500.1726444581951036No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG29950.16414925469661437No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28800.1578463617783804No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27600.15126943003761456No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA27470.1505569290990316No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG26440.14491172935487423No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG26340.14436365170981041No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG24740.13559440938878928No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC23640.12956555529308728No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG23310.12775689906437665No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22370.12260496920077674No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT21100.11564438310846621No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA20860.11432899676031305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20730.11361649582173007No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT20510.11241072500258967No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20120.11027322218684077No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT19740.10819052713559824No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19670.10780687278405356No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA19470.10671071749392594No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18900.10358667491706215No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC18440.10106551774976856No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG18310.10035301681118561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA1200.021.3342115
ACTAGAC751.5117384E-619.1971053
CGTTTAT6050.017.18984831
ATCGTTT6100.017.04894629
CAAGACG9650.016.9079784
ACCGTCG5600.016.8555268
GACGGAC9750.016.7368567
AAGACGG10050.016.3932915
ATACCGT6000.016.2628776
CGCAAGA9950.016.2454362
CATCGTT6350.016.1248828
GCGCAAG9700.016.0164641
CCGTCGT6000.016.000229
TCGTTTA6450.015.875751530
CGTCGTA6150.015.87013610
TACCGTC6050.015.8662477
GCATCGT6400.015.74805727
AGACGGA10800.015.7000466
GTATTAG4500.015.66054251
TCTAGCG6850.015.64856328
Produced by FastQC (version 0.11.5)