##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062803_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1824559
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26482234885252	32.0	32.0	32.0	32.0	32.0
2	30.94664518933068	32.0	32.0	32.0	32.0	32.0
3	30.991083324792456	32.0	32.0	32.0	32.0	32.0
4	31.045926166268124	32.0	32.0	32.0	32.0	32.0
5	30.98897432201425	32.0	32.0	32.0	32.0	32.0
6	34.63674071378344	36.0	36.0	36.0	32.0	36.0
7	34.567990950141926	36.0	36.0	36.0	32.0	36.0
8	34.55663532941385	36.0	36.0	36.0	32.0	36.0
9	34.657296913939206	36.0	36.0	36.0	32.0	36.0
10	34.42339765389883	36.0	36.0	36.0	32.0	36.0
11	34.641889903258814	36.0	36.0	36.0	32.0	36.0
12	34.48070081592319	36.0	36.0	36.0	32.0	36.0
13	34.56289930881928	36.0	36.0	36.0	32.0	36.0
14	34.46441578485541	36.0	36.0	36.0	32.0	36.0
15	34.418326291449056	36.0	36.0	36.0	32.0	36.0
16	34.43023437444336	36.0	36.0	36.0	32.0	36.0
17	34.34984837431949	36.0	36.0	36.0	32.0	36.0
18	34.38048481852327	36.0	36.0	36.0	32.0	36.0
19	34.36181290931123	36.0	36.0	36.0	32.0	36.0
20	34.344696992533535	36.0	36.0	36.0	32.0	36.0
21	34.32302929091359	36.0	36.0	36.0	32.0	36.0
22	34.29955731768608	36.0	36.0	36.0	32.0	36.0
23	34.246194833929735	36.0	36.0	36.0	32.0	36.0
24	34.2284886375283	36.0	36.0	36.0	32.0	36.0
25	34.20498432771974	36.0	36.0	36.0	32.0	36.0
26	34.15426522244553	36.0	36.0	36.0	32.0	36.0
27	34.160235432233215	36.0	36.0	36.0	32.0	36.0
28	34.141987735118455	36.0	36.0	36.0	32.0	36.0
29	34.11379133259051	36.0	36.0	36.0	32.0	36.0
30	34.08237332966487	36.0	36.0	36.0	32.0	36.0
31	34.10575158161507	36.0	36.0	36.0	32.0	36.0
32	34.05443123516422	36.0	36.0	36.0	32.0	36.0
33	34.01242820867947	36.0	36.0	36.0	32.0	36.0
34	34.01461503848327	36.0	36.0	36.0	32.0	36.0
35	33.96316589378584	36.0	36.0	36.0	32.0	36.0
36	33.92605665259386	36.0	36.0	36.0	32.0	36.0
37	33.92826924204698	36.0	36.0	36.0	32.0	36.0
38	33.30970168681857	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	2.0
9	7.0
10	17.0
11	18.0
12	10.0
13	12.0
14	260.0
15	559.0
16	704.0
17	807.0
18	1070.0
19	1395.0
20	1802.0
21	2508.0
22	3610.0
23	5370.0
24	7647.0
25	11124.0
26	16290.0
27	23261.0
28	32678.0
29	44409.0
30	60056.0
31	81491.0
32	111833.0
33	168615.0
34	388528.0
35	860474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.69609577047746	17.06531705917117	11.445891227679319	25.792695942672044
2	17.077272949121014	19.77704695800382	36.72056782393203	26.425112268943142
3	19.104858367724823	23.67111997399603	28.09628922726448	29.127732431014664
4	12.781391545318618	15.056256385297045	35.30515690839812	36.857195160986215
5	15.30215055295705	35.809463573015655	32.66272195835256	16.225663915674733
6	34.57917135959179	35.012866150924474	16.535538802612145	13.872423686871594
7	30.80439207742368	30.167854351574462	20.410367880878546	18.617385690123307
8	28.72341066900075	31.646202338430644	19.462532548996826	20.16785444357178
9	27.773292665882916	14.169756461164503	18.510814965840986	39.5461359071116
10	16.7008303417281	25.482118878627606	30.16075196623825	27.656298813406043
11	38.24100067634005	20.90877301830722	21.65905007656783	19.1911762287849
12	25.837701615113268	23.433063892013006	27.41653128829783	23.3127032045759
13	29.48201908134375	18.654000586527946	25.143740458642927	26.720239873485376
14	24.517908148079936	19.713406047929364	24.09908391448553	31.669601889505167
15	25.787013408488257	26.322717023829824	22.16213312223877	25.728136445443152
16	26.627058386310644	25.164961416733266	23.031014610006984	25.176965586949112
17	24.443635570266345	25.252424475484993	24.754948583194142	25.548991371054523
18	25.819233193565182	23.722436898791415	25.937789470799423	24.52054043684398
19	26.169479336754687	24.387874961015072	25.02467903550876	24.417966666721476
20	26.328566229347178	23.291263987792473	24.57389851615393	25.80627126670642
21	28.01842100503223	23.4713794212906	23.748670139149624	24.761529434527546
22	26.573676484936048	23.516489057904366	24.382461952333394	25.527372504826186
23	24.5816112871222	23.239456692136507	25.244356987031207	26.934575033710082
24	25.227825238514185	24.683072824594436	24.729775285518127	25.359326651373255
25	25.382562726254143	23.590618963358615	25.020007937395437	26.006810372991808
26	24.86557334632878	24.982992815046796	25.461167430185572	24.690266408438852
27	25.92536764484212	23.988219578893247	24.484935454890778	25.601477321373856
28	24.706939880676696	23.935038134675835	25.622775020485573	25.735246964161902
29	24.61435918012066	24.233459733408758	25.56844405124849	25.5837370352221
30	24.576020362861456	24.55552157360545	25.83631223492709	25.032145828606005
31	25.543590862302008	24.356378553004152	24.030744742776147	26.069285841917694
32	25.05035800085177	24.34553782893024	24.041005526636436	26.563098643581558
33	24.51630832172225	23.822000146897125	25.10444495359037	26.55724657779025
34	25.72291200626205	24.11442491269364	25.14028491665127	25.02237816439304
35	26.589107724995575	23.690158185815847	25.189681002627385	24.531053086561194
36	24.70725854105616	24.946363164951492	24.51025211779164	25.8361261762007
37	26.090965050420117	24.550488908231934	24.566110730766933	24.79243531058102
38	24.817670679208923	24.3200401656174	25.065855607674877	25.796433547498804
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	710.0
1	573.5
2	437.0
3	437.0
4	910.5
5	1384.0
6	1384.0
7	1627.0
8	1870.0
9	1742.0
10	1614.0
11	1614.0
12	2153.5
13	2693.0
14	3421.0
15	4149.0
16	4149.0
17	6025.0
18	7901.0
19	7901.0
20	9383.0
21	10865.0
22	10935.5
23	11006.0
24	11006.0
25	12453.5
26	13901.0
27	13901.0
28	18417.0
29	22933.0
30	28612.0
31	34291.0
32	34291.0
33	45670.0
34	57049.0
35	57049.0
36	63762.5
37	70476.0
38	81676.5
39	92877.0
40	92877.0
41	97487.5
42	102098.0
43	119546.5
44	136995.0
45	136995.0
46	141290.5
47	145586.0
48	145586.0
49	156163.5
50	166741.0
51	169915.0
52	173089.0
53	173089.0
54	165760.5
55	158432.0
56	158432.0
57	156340.5
58	154249.0
59	138690.0
60	123131.0
61	123131.0
62	120136.0
63	117141.0
64	96436.5
65	75732.0
66	75732.0
67	63872.0
68	52012.0
69	52012.0
70	41796.5
71	31581.0
72	24953.0
73	18325.0
74	18325.0
75	13666.5
76	9008.0
77	9008.0
78	9031.0
79	9054.0
80	7181.5
81	5309.0
82	5309.0
83	5636.5
84	5964.0
85	5964.0
86	3995.5
87	2027.0
88	1740.0
89	1453.0
90	1453.0
91	1052.0
92	651.0
93	568.5
94	486.0
95	486.0
96	434.0
97	382.0
98	382.0
99	669.5
100	957.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12068669744305337
2	0.056780844028611846
3	0.012167323720416824
4	0.003014427047851015
5	2.740388225319104E-4
6	2.1923105802552835E-4
7	5.480776450638209E-5
8	1.0961552901276418E-4
9	2.1923105802552835E-4
10	4.932698805574389E-4
11	0.001808656228710609
12	0.0023567338737744297
13	0.014469249829684871
14	0.009098088908059427
15	0.022800030034654948
16	0.010577898549731743
17	0.01918271757723373
18	0.0059740463311956475
19	0.007453855972867965
20	0.006083661860208412
21	0.006576931740765851
22	0.007508663737374346
23	0.009810589846642393
24	0.01337309453955723
25	0.015510597355306133
26	0.01808656228710609
27	0.009646166553123248
28	0.005700007508663737
29	0.010577898549731743
30	0.0033432736348893077
31	0.006412508447246704
32	0.006686547269778615
33	0.00838558796947646
34	0.012934632423506173
35	0.017100022525991212
36	0.01386636442011467
37	0.010303859727199832
38	0.006193277389221177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1824559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.45371744994846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30008462321655	33.15522318624409
2	13.069081808491736	11.358003764729228
3	4.421329747781758	5.763696408394813
4	1.9583007583213983	3.403817913364714
5	1.0568645996869737	2.2962347848825324
6	0.6711683744967003	1.7498856544032486
7	0.4245225833010442	1.291295907012005
8	0.3171197163198986	1.1024024440618148
9	0.24020630064902712	0.9394071046290176
>10	1.2903129391568535	10.062101775437085
>50	0.10627752596739021	3.267947710074232
>100	0.1118666352540111	10.715550417245183
>500	0.02085624582257522	6.167217552669872
>1k	0.011881740044376185	8.407490279560612
>5k	1.264014898337892E-4	0.31972509729156345
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5821	0.31903599719165016	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3639	0.1994454550387244	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3477	0.19056659718869054	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3227	0.176864656062095	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3207	0.17576850077196735	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3150	0.1726444581951036	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2995	0.16414925469661437	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2880	0.1578463617783804	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2760	0.15126943003761456	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2747	0.1505569290990316	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2644	0.14491172935487423	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2634	0.14436365170981041	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2474	0.13559440938878928	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2364	0.12956555529308728	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2331	0.12775689906437665	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2237	0.12260496920077674	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2110	0.11564438310846621	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2086	0.11432899676031305	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2073	0.11361649582173007	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	2051	0.11241072500258967	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2012	0.11027322218684077	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1974	0.10819052713559824	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1967	0.10780687278405356	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1947	0.10671071749392594	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1890	0.10358667491706215	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1844	0.10106551774976856	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1831	0.10035301681118561	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.480776450638209E-5	5.480776450638209E-5
2	0.0	0.0	0.0	5.480776450638209E-5	5.480776450638209E-5
3	0.0	0.0	0.0	5.480776450638209E-5	5.480776450638209E-5
4	0.0	0.0	0.0	5.480776450638209E-5	5.480776450638209E-5
5	0.0	5.480776450638209E-5	0.0	5.480776450638209E-5	5.480776450638209E-5
6	0.0	5.480776450638209E-5	0.0	5.480776450638209E-5	5.480776450638209E-5
7	0.0	5.480776450638209E-5	0.0	5.480776450638209E-5	5.480776450638209E-5
8	0.0	5.480776450638209E-5	0.0	5.480776450638209E-5	5.480776450638209E-5
9	0.0	5.480776450638209E-5	0.0	5.480776450638209E-5	5.480776450638209E-5
10	0.0	5.480776450638209E-5	0.0	5.480776450638209E-5	5.480776450638209E-5
11	0.0	5.480776450638209E-5	0.0	5.480776450638209E-5	5.480776450638209E-5
12	0.0	5.480776450638209E-5	0.0	5.480776450638209E-5	1.0961552901276418E-4
13	0.0	5.480776450638209E-5	0.0	5.480776450638209E-5	1.0961552901276418E-4
14	0.0	5.480776450638209E-5	0.0	1.0961552901276418E-4	1.0961552901276418E-4
15	0.0	5.480776450638209E-5	0.0	1.0961552901276418E-4	1.0961552901276418E-4
16	0.0	5.480776450638209E-5	0.0	1.0961552901276418E-4	1.6442329351914627E-4
17	0.0	5.480776450638209E-5	0.0	1.6442329351914627E-4	1.6442329351914627E-4
18	0.0	5.480776450638209E-5	0.0	2.1923105802552835E-4	1.6442329351914627E-4
19	0.0	5.480776450638209E-5	0.0	2.1923105802552835E-4	1.6442329351914627E-4
20	0.0	5.480776450638209E-5	0.0	3.8365435154467465E-4	3.2884658703829253E-4
21	0.0	5.480776450638209E-5	0.0	4.932698805574388E-4	3.2884658703829253E-4
22	0.0	5.480776450638209E-5	0.0	7.673087030893493E-4	3.8365435154467465E-4
23	0.0	5.480776450638209E-5	0.0	0.0013701941126595522	3.8365435154467465E-4
24	0.0	5.480776450638209E-5	0.0	0.002521157167293576	3.8365435154467465E-4
25	0.0	5.480776450638209E-5	0.0	0.003014427047851015	3.8365435154467465E-4
26	0.0	5.480776450638209E-5	0.0	0.004110582337978657	3.8365435154467465E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	120	0.0	21.33421	15
ACTAGAC	75	1.5117384E-6	19.197105	3
CGTTTAT	605	0.0	17.189848	31
ATCGTTT	610	0.0	17.048946	29
CAAGACG	965	0.0	16.907978	4
ACCGTCG	560	0.0	16.855526	8
GACGGAC	975	0.0	16.736856	7
AAGACGG	1005	0.0	16.393291	5
ATACCGT	600	0.0	16.262877	6
CGCAAGA	995	0.0	16.245436	2
CATCGTT	635	0.0	16.12488	28
GCGCAAG	970	0.0	16.016464	1
CCGTCGT	600	0.0	16.00022	9
TCGTTTA	645	0.0	15.8757515	30
CGTCGTA	615	0.0	15.870136	10
TACCGTC	605	0.0	15.866247	7
GCATCGT	640	0.0	15.748057	27
AGACGGA	1080	0.0	15.700046	6
GTATTAG	450	0.0	15.6605425	1
TCTAGCG	685	0.0	15.648563	28
>>END_MODULE