FastQCFastQC Report
Thu 2 Feb 2017
SRR4062803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824559
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT63040.3455081474482327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT42540.23315223021014939No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC34170.18727813131830762No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT33900.1857983216766353No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG33170.1817973548676694No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA30920.16946560785373344No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC30340.16628675751236327No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC30290.16601271868983133No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG28600.15675020648825277No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA28470.1560377055496698No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG27530.1508857756860699No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC27430.15033769804100608No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG27150.14880308063482736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT26780.14677519334809125No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA23800.1304424795251894No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG22120.12123477508811718No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21940.1202482353270023No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT21870.11986458097545763No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT21030.11526072875692153No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC20490.11230110947357691No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA20360.11158860853499393No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT20250.11098572312542372No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19950.10934149019023226No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT19620.10753283396152166No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19300.10577898549731743No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19250.10550494667478552No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19110.10473763797169616No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG18730.10265494292045366No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT18420.1009559022207558No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT18350.10057224786921112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG350.002065665322.859699
GCTAGTA350.00206764122.855932
TCTAGCG6750.018.48790728
CTAGCGG6700.018.38708129
ACTTATA450.0088549217.7768342
TAGCGGC7000.017.59906630
ACCGTCG5700.016.843988
TACCGTC5700.016.843987
ATACCGT6050.016.6630126
GTATAGA1357.907147E-915.4091231
ATCGTTT5150.015.22249229
GTCGTAG6350.014.8673911
CGTCGTA6150.014.83091910
AAGACGG7350.014.804375
TCAGATA6600.014.7870942
GTTCTAG1308.306779E-814.7708761
CATCGTT5200.014.76844628
GTCCTAT6000.014.66831
CGGTCCA9000.014.579410
CCGTCGT6400.014.5016159
Produced by FastQC (version 0.11.5)