##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062803_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1824559
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17969876556472	32.0	32.0	32.0	32.0	32.0
2	31.300375049532516	32.0	32.0	32.0	32.0	32.0
3	31.370024756667227	32.0	32.0	32.0	32.0	32.0
4	31.470458888969883	32.0	32.0	32.0	32.0	32.0
5	31.387781376212004	32.0	32.0	32.0	32.0	32.0
6	34.911039873196756	36.0	36.0	36.0	36.0	36.0
7	34.937229215388484	36.0	36.0	36.0	36.0	36.0
8	34.87942182193067	36.0	36.0	36.0	32.0	36.0
9	34.98238314025471	36.0	36.0	36.0	36.0	36.0
10	34.83179168226404	36.0	36.0	36.0	32.0	36.0
11	35.005479680295345	36.0	36.0	36.0	36.0	36.0
12	34.89245127178677	36.0	36.0	36.0	32.0	36.0
13	34.9511843683871	36.0	36.0	36.0	36.0	36.0
14	34.890103855232965	36.0	36.0	36.0	32.0	36.0
15	34.85809228421772	36.0	36.0	36.0	32.0	36.0
16	34.86638469898754	36.0	36.0	36.0	32.0	36.0
17	34.82569431846271	36.0	36.0	36.0	32.0	36.0
18	34.83149352802513	36.0	36.0	36.0	32.0	36.0
19	34.82421670113162	36.0	36.0	36.0	32.0	36.0
20	34.80537324361668	36.0	36.0	36.0	32.0	36.0
21	34.789077250995994	36.0	36.0	36.0	32.0	36.0
22	34.77740374523378	36.0	36.0	36.0	32.0	36.0
23	34.7110929271128	36.0	36.0	36.0	32.0	36.0
24	34.68648917354824	36.0	36.0	36.0	32.0	36.0
25	34.66754541782425	36.0	36.0	36.0	32.0	36.0
26	34.60096330126896	36.0	36.0	36.0	32.0	36.0
27	34.58671492673024	36.0	36.0	36.0	32.0	36.0
28	34.55080104288214	36.0	36.0	36.0	32.0	36.0
29	34.50641881134017	36.0	36.0	36.0	32.0	36.0
30	34.48749423833376	36.0	36.0	36.0	32.0	36.0
31	34.49040836717256	36.0	36.0	36.0	32.0	36.0
32	34.452470432581244	36.0	36.0	36.0	32.0	36.0
33	34.40696025724572	36.0	36.0	36.0	32.0	36.0
34	34.399311285631214	36.0	36.0	36.0	32.0	36.0
35	34.36080883106548	36.0	36.0	36.0	32.0	36.0
36	34.33028255046836	36.0	36.0	36.0	32.0	36.0
37	34.32072407633844	36.0	36.0	36.0	32.0	36.0
38	33.874651902185676	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	9.0
21	44.0
22	185.0
23	465.0
24	1316.0
25	3302.0
26	6756.0
27	13057.0
28	22725.0
29	35351.0
30	53237.0
31	75357.0
32	107423.0
33	170315.0
34	413171.0
35	921845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.025400498680945	18.353410110539663	12.143960824659768	26.47722856611962
2	16.045630752417434	20.723857107388692	36.00727627881587	27.223235861378008
3	18.08338343676472	24.368189792711554	28.428348987344336	29.120077783179386
4	12.283762342050528	15.890998078983642	35.3938905650967	36.43134901386914
5	14.667497552831973	36.32233121665631	32.47520769413743	16.53496353637429
6	34.670042832361865	35.31524125060631	16.251140689099532	13.76357522793229
7	30.506573946137983	30.354481732766036	20.431930924375365	18.70701339672062
8	28.563113309305198	31.870379626950097	19.301868757289554	20.264638306455147
9	27.73317153407294	13.974588965750845	18.37709641580656	39.91514308436965
10	16.514966246268237	25.721456214419092	30.170470458565152	27.59310708074752
11	38.373448641206195	20.754156460388863	21.604111707151034	19.268283191253907
12	25.647939877843985	23.53098185198339	27.380200728726834	23.44087754144579
13	29.48331482652747	18.63732177836337	25.128003884784995	26.751359510324168
14	24.34127918033892	19.666505714531564	24.188255901837103	31.803959203292415
15	25.78014742192497	26.363192420743864	22.06423579615677	25.792424361174398
16	26.585123629942153	25.187415253447686	22.959916867537235	25.267544249072927
17	24.375314802097385	25.43787293258261	24.60972761089118	25.577084654428823
18	25.899737964077897	23.651468656261596	25.92615530656997	24.522638073090537
19	26.1325065399365	24.41587254783211	24.98318771823767	24.46843319399373
20	26.334911614258573	23.19102862664348	24.690569063538092	25.78349069555986
21	28.009569456662035	23.500908440688278	23.743268906664913	24.746253195984774
22	26.454447348646987	23.58005413910978	24.48202551959131	25.483472992651922
23	24.533447626104792	23.091835045715282	25.392013609901376	26.98270371827855
24	25.1406504256645	24.63866611055055	24.809940374632994	25.410743089151953
25	25.39021800908385	23.53837123203057	25.07836148719936	25.993049271686225
26	25.035433239173543	24.856650213366745	25.433283019777942	24.67463352768177
27	25.980607895505592	23.872096075924183	24.55319291203289	25.59410311653733
28	24.72602968717372	23.830525622903945	25.720680997435547	25.72276369248679
29	24.724221030945014	24.04137109296	25.655185718850422	25.579222157244573
30	24.686732520022648	24.33311282342747	25.91541298472672	25.064741671823164
31	25.536746139752125	24.152740470436964	24.21779728690604	26.092716102904866
32	25.18477067609214	24.17236165013025	24.1538914334916	26.48897624028601
33	24.533106356111258	23.663252325630467	25.250430377970783	26.553210940287485
34	25.67310785784401	23.794516921623252	25.406851737872	25.125523482660743
35	26.638820668446456	23.534673310098494	25.198637040512256	24.62786898094279
36	24.776014368403544	24.717041213794676	24.63060936916811	25.87633504863367
37	26.223158582430056	24.39838887095457	24.52477557590629	24.85367697070909
38	24.988394455865773	23.92665844184814	25.160271605357785	25.9246754969283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	423.0
1	334.0
2	245.0
3	245.0
4	803.0
5	1361.0
6	1361.0
7	1590.0
8	1819.0
9	1766.5
10	1714.0
11	1714.0
12	2181.0
13	2648.0
14	3470.5
15	4293.0
16	4293.0
17	6459.0
18	8625.0
19	8625.0
20	9769.5
21	10914.0
22	11028.0
23	11142.0
24	11142.0
25	12339.0
26	13536.0
27	13536.0
28	18291.0
29	23046.0
30	28647.0
31	34248.0
32	34248.0
33	45790.5
34	57333.0
35	57333.0
36	63874.5
37	70416.0
38	81577.0
39	92738.0
40	92738.0
41	97453.5
42	102169.0
43	119010.5
44	135852.0
45	135852.0
46	140666.0
47	145480.0
48	145480.0
49	156862.5
50	168245.0
51	170569.5
52	172894.0
53	172894.0
54	166242.5
55	159591.0
56	159591.0
57	157248.5
58	154906.0
59	139313.0
60	123720.0
61	123720.0
62	120809.0
63	117898.0
64	97233.5
65	76569.0
66	76569.0
67	64483.5
68	52398.0
69	52398.0
70	42093.5
71	31789.0
72	24998.5
73	18208.0
74	18208.0
75	13442.5
76	8677.0
77	8677.0
78	8853.5
79	9030.0
80	6937.5
81	4845.0
82	4845.0
83	5002.0
84	5159.0
85	5159.0
86	3300.5
87	1442.0
88	1184.0
89	926.0
90	926.0
91	545.0
92	164.0
93	108.0
94	52.0
95	52.0
96	36.5
97	21.0
98	21.0
99	22.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008001933617931785
2	0.0
3	0.0
4	2.1923105802552835E-4
5	5.480776450638209E-5
6	2.1923105802552835E-4
7	4.384621160510567E-4
8	0.001699040699697845
9	0.004165390102485039
10	0.0010961552901276417
11	0.00602885409570203
12	3.8365435154467465E-4
13	2.740388225319104E-4
14	0.0
15	0.0
16	5.480776450638209E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	2.1923105802552835E-4
22	0.0
23	2.740388225319104E-4
24	0.0
25	1.0961552901276418E-4
26	5.480776450638209E-5
27	1.0961552901276418E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1824559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.4839754436652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48531464478785	30.89946963638055
2	14.23377864697843	11.809474477236385
3	4.601691873144911	5.726894179945715
4	2.0732809782409043	3.4403174875665528
5	1.1393863313489385	2.3633137295263573
6	0.7203075945599294	1.7928733538765795
7	0.48176854304548355	1.398997208646044
8	0.3587324382337292	1.1905318126827331
9	0.26253398917575954	0.9801858204085208
>10	1.3753683855674506	10.317876454326527
>50	0.1100110682064361	3.1967287575257366
>100	0.12148610620860796	10.997613709848048
>500	0.023209471123276752	6.520829895150944
>1k	0.012997303829034981	9.018057796628371
>5k	1.3262554927586715E-4	0.3468356802510234
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6304	0.3455081474482327	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4254	0.23315223021014939	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3417	0.18727813131830762	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3390	0.1857983216766353	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3317	0.1817973548676694	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3092	0.16946560785373344	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3034	0.16628675751236327	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3029	0.16601271868983133	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2860	0.15675020648825277	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2847	0.1560377055496698	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2753	0.1508857756860699	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2743	0.15033769804100608	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2715	0.14880308063482736	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2678	0.14677519334809125	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2380	0.1304424795251894	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2212	0.12123477508811718	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2194	0.1202482353270023	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2187	0.11986458097545763	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2103	0.11526072875692153	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2049	0.11230110947357691	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2036	0.11158860853499393	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	2025	0.11098572312542372	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1995	0.10934149019023226	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1962	0.10753283396152166	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1930	0.10577898549731743	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1925	0.10550494667478552	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1911	0.10473763797169616	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1873	0.10265494292045366	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1842	0.1009559022207558	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1835	0.10057224786921112	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0961552901276418E-4
2	0.0	0.0	0.0	0.0	1.0961552901276418E-4
3	0.0	0.0	0.0	0.0	1.0961552901276418E-4
4	0.0	0.0	0.0	0.0	1.0961552901276418E-4
5	0.0	0.0	0.0	0.0	1.0961552901276418E-4
6	0.0	0.0	0.0	0.0	1.0961552901276418E-4
7	0.0	0.0	0.0	0.0	1.0961552901276418E-4
8	0.0	0.0	0.0	0.0	1.0961552901276418E-4
9	0.0	0.0	0.0	0.0	1.0961552901276418E-4
10	0.0	0.0	0.0	0.0	1.0961552901276418E-4
11	0.0	0.0	0.0	0.0	1.0961552901276418E-4
12	0.0	0.0	0.0	0.0	2.7403882253191047E-4
13	0.0	0.0	0.0	0.0	5.480776450638209E-4
14	0.0	0.0	0.0	5.480776450638209E-5	5.480776450638209E-4
15	0.0	0.0	0.0	5.480776450638209E-5	6.02885409570203E-4
16	0.0	0.0	0.0	5.480776450638209E-5	6.02885409570203E-4
17	0.0	0.0	0.0	1.6442329351914627E-4	6.02885409570203E-4
18	0.0	0.0	0.0	1.6442329351914627E-4	6.576931740765851E-4
19	0.0	0.0	0.0	1.6442329351914627E-4	6.576931740765851E-4
20	0.0	0.0	0.0	2.7403882253191047E-4	7.673087030893493E-4
21	0.0	0.0	0.0	4.384621160510567E-4	7.673087030893493E-4
22	0.0	0.0	0.0	7.673087030893493E-4	8.221164675957314E-4
23	0.0	0.0	0.0	0.0014250018771659342	8.221164675957314E-4
24	0.0	0.0	0.0	0.0026307726963063402	8.221164675957314E-4
25	0.0	0.0	0.0	0.003069234812357397	8.221164675957314E-4
26	0.0	0.0	0.0	0.004275005631497803	8.221164675957314E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAACG	35	0.0020656653	22.85969	9
GCTAGTA	35	0.002067641	22.85593	2
TCTAGCG	675	0.0	18.487907	28
CTAGCGG	670	0.0	18.387081	29
ACTTATA	45	0.00885492	17.776834	2
TAGCGGC	700	0.0	17.599066	30
ACCGTCG	570	0.0	16.84398	8
TACCGTC	570	0.0	16.84398	7
ATACCGT	605	0.0	16.663012	6
GTATAGA	135	7.907147E-9	15.409123	1
ATCGTTT	515	0.0	15.222492	29
GTCGTAG	635	0.0	14.86739	11
CGTCGTA	615	0.0	14.830919	10
AAGACGG	735	0.0	14.80437	5
TCAGATA	660	0.0	14.787094	2
GTTCTAG	130	8.306779E-8	14.770876	1
CATCGTT	520	0.0	14.768446	28
GTCCTAT	600	0.0	14.6683	1
CGGTCCA	900	0.0	14.5794	10
CCGTCGT	640	0.0	14.501615	9
>>END_MODULE