FastQCFastQC Report
Thu 2 Feb 2017
SRR4062802_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062802_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139736
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT52800.24675941331080095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49960.23348674789787152No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC45570.21297019819267426No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT37540.17544220408498992No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC35870.16763750294428845No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA33820.15805688178354713No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC32740.15300953014764437No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC32330.1510934059154961No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG31120.14543850269379024No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG30000.14020421210840964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29800.13926951736102025No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG29070.13585788153304892No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG29060.13581114679567946No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC28950.1352970646846153No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG26700.12478174877648457No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG26290.12286562454433632No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC25490.11912684555477873No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG24760.11571520972680743No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA24210.11314479917148657No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC24180.11300459495937817No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA23890.11164928757566353No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT22850.10678887488923869No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG22130.10342397379863684No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21660.10122744114227175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG7650.020.70397628
CTAGCGG7800.020.30724729
TAGCGGC8000.019.99956130
CATCGTT7300.017.75179128
CGGTCCA9300.017.37677410
CGCCGGT9150.017.3098817
CGGCATC7600.017.2627825
TAGGACG5500.017.1600514
AAGACGG10350.017.0016785
CGTTTAT7250.016.99273131
ATCGTTT7450.016.96607229
CAAGACG10100.016.6301884
GGACGTG5500.016.579136
TCGTTTA7450.016.5365530
AGCGGCG9850.016.40573131
GCCGGTC10200.016.3122258
CCGGTCC10050.016.2392089
GCATCGT7750.016.10174427
GTCCTAT7100.016.014621
GTATCAA40350.015.9153991
Produced by FastQC (version 0.11.5)