##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062802_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2139736
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.295112107288002	32.0	32.0	32.0	32.0	32.0
2	30.943794468102606	32.0	32.0	32.0	32.0	32.0
3	30.980971016985272	32.0	32.0	32.0	32.0	32.0
4	31.044629337450974	32.0	32.0	32.0	32.0	32.0
5	30.97387715119996	32.0	32.0	32.0	32.0	32.0
6	34.63593966732345	36.0	36.0	36.0	32.0	36.0
7	34.568593508731915	36.0	36.0	36.0	32.0	36.0
8	34.55331685778059	36.0	36.0	36.0	32.0	36.0
9	34.6770961464405	36.0	36.0	36.0	32.0	36.0
10	34.41523673948562	36.0	36.0	36.0	32.0	36.0
11	34.64680923254084	36.0	36.0	36.0	32.0	36.0
12	34.47751778724104	36.0	36.0	36.0	32.0	36.0
13	34.566977888861054	36.0	36.0	36.0	32.0	36.0
14	34.46624022776641	36.0	36.0	36.0	32.0	36.0
15	34.41327902133721	36.0	36.0	36.0	32.0	36.0
16	34.4267923706476	36.0	36.0	36.0	32.0	36.0
17	34.353517443273375	36.0	36.0	36.0	32.0	36.0
18	34.38106990768955	36.0	36.0	36.0	32.0	36.0
19	34.36934462943092	36.0	36.0	36.0	32.0	36.0
20	34.3525523709467	36.0	36.0	36.0	32.0	36.0
21	34.33560542048178	36.0	36.0	36.0	32.0	36.0
22	34.30585034789338	36.0	36.0	36.0	32.0	36.0
23	34.262776342502065	36.0	36.0	36.0	32.0	36.0
24	34.24628271898963	36.0	36.0	36.0	32.0	36.0
25	34.22321678936093	36.0	36.0	36.0	32.0	36.0
26	34.17074536297936	36.0	36.0	36.0	32.0	36.0
27	34.18698942299424	36.0	36.0	36.0	32.0	36.0
28	34.17398127619482	36.0	36.0	36.0	32.0	36.0
29	34.13207657393249	36.0	36.0	36.0	32.0	36.0
30	34.10695057708054	36.0	36.0	36.0	32.0	36.0
31	34.12873831164218	36.0	36.0	36.0	32.0	36.0
32	34.08151379422508	36.0	36.0	36.0	32.0	36.0
33	34.04497330511801	36.0	36.0	36.0	32.0	36.0
34	34.05962511263072	36.0	36.0	36.0	32.0	36.0
35	34.018356937491355	36.0	36.0	36.0	32.0	36.0
36	33.975799350947966	36.0	36.0	36.0	32.0	36.0
37	33.99182656178145	36.0	36.0	36.0	32.0	36.0
38	33.39446688750388	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	4.0
9	4.0
10	16.0
11	25.0
12	11.0
13	7.0
14	136.0
15	401.0
16	593.0
17	667.0
18	856.0
19	1194.0
20	1673.0
21	2523.0
22	3725.0
23	5701.0
24	8773.0
25	13201.0
26	19277.0
27	27832.0
28	39073.0
29	52525.0
30	71191.0
31	96138.0
32	132108.0
33	199232.0
34	455970.0
35	1006875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.97419746691392	16.870894216726864	11.224115930310418	25.930792386048797
2	17.41480223119701	18.64673945317998	36.914583160906076	27.02387515471693
3	19.951931840808577	22.123973223174797	27.559264899306463	30.36483003671016
4	12.808068949304538	14.309710098665255	35.05773583547659	37.82448511655362
5	15.288215866185173	35.745581341223414	32.79334384243268	16.172858950158737
6	35.87422905028716	34.5907499587565	15.919150584816503	13.615870406139837
7	31.86734251328201	29.582294264339154	19.459643619586718	19.09071960279212
8	28.999306920929634	32.378476319642644	18.489835385542175	20.132381373885547
9	27.159662985831872	14.278189978931941	18.503906096651356	40.05824093858483
10	16.76898515883357	25.455105424811798	29.986297298156213	27.78961211819842
11	39.001950279783124	20.782654334056332	21.092745548076287	19.12264983808425
12	25.79432438495476	23.124953264039483	27.25613175801989	23.824590592985867
13	29.414228249153744	18.572155065311666	25.193158564367955	26.820458121166624
14	23.85555908691507	19.627680457664194	23.842285329693954	32.67447512572679
15	25.44087598815823	27.182268943585168	21.566402877327906	25.810452190928697
16	26.70868853355494	25.440600956943165	23.112135847229357	24.738574662272537
17	24.526243359927903	25.868888843183047	24.303505284945803	25.30136251194325
18	25.771071498677333	24.102924818425702	25.813181779942234	24.31282190295473
19	25.930895750986526	24.286506831484136	24.986878259695015	24.795719157834323
20	26.379241528160634	23.629171188501875	24.299664706503684	25.691922576833807
21	27.644980370162646	23.524116657319123	23.90535614133483	24.925546831183397
22	26.561995375743074	23.78697384121328	24.69920596825462	24.951824814789024
23	24.466709791039975	23.7118774619686	25.648501459186708	26.172911287804713
24	25.151301275100025	24.55731406349325	25.10745802639943	25.183926635007293
25	25.09654657279532	24.092752019041015	25.051253118891342	25.759448289272324
26	24.665477502643366	25.71847663601204	25.42782325956216	24.188222601782435
27	25.7110821123635	24.15089859765297	24.802201952180734	25.335817337802798
28	24.68183691916251	24.304341039041404	25.600735829532823	25.413086212263263
29	24.436430058434595	24.55300011311128	25.652095877234064	25.358473951220063
30	24.23970930164628	25.015621531307325	25.913141014406392	24.831528152640004
31	25.544514103945854	24.615187599258558	24.20067357944003	25.63962471735556
32	25.100800939235274	24.412311946132313	24.206667280484837	26.280219834147577
33	24.384855331284953	24.21201750999496	25.301624899863427	26.10150225885665
34	25.724616681841724	24.39419745769525	25.151672652556044	24.729513207906983
35	26.115436858986644	24.01212310447951	25.39985771618586	24.47258232034799
36	24.235305335661693	25.10811361605223	24.91768781761004	25.738893230676034
37	25.892531637760037	24.76303228930439	24.57944073750708	24.764995335428498
38	24.552746429963534	24.304477349652785	25.223381413656853	25.91939480672682
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	78.0
2	125.0
3	125.0
4	348.0
5	571.0
6	571.0
7	803.0
8	1035.0
9	1098.5
10	1162.0
11	1162.0
12	1606.0
13	2050.0
14	2934.0
15	3818.0
16	3818.0
17	5506.0
18	7194.0
19	7194.0
20	8863.5
21	10533.0
22	11344.5
23	12156.0
24	12156.0
25	14669.0
26	17182.0
27	17182.0
28	22523.0
29	27864.0
30	35624.5
31	43385.0
32	43385.0
33	55901.5
34	68418.0
35	68418.0
36	78307.0
37	88196.0
38	101284.0
39	114372.0
40	114372.0
41	120072.5
42	125773.0
43	144548.5
44	163324.0
45	163324.0
46	165370.5
47	167417.0
48	167417.0
49	178561.0
50	189705.0
51	198853.0
52	208001.0
53	208001.0
54	197162.5
55	186324.0
56	186324.0
57	181367.0
58	176410.0
59	158100.5
60	139791.0
61	139791.0
62	135758.5
63	131726.0
64	111296.0
65	90866.0
66	90866.0
67	76524.5
68	62183.0
69	62183.0
70	50092.0
71	38001.0
72	29965.0
73	21929.0
74	21929.0
75	16603.0
76	11277.0
77	11277.0
78	10818.0
79	10359.0
80	8228.0
81	6097.0
82	6097.0
83	6122.5
84	6148.0
85	6148.0
86	4154.0
87	2160.0
88	1856.0
89	1552.0
90	1552.0
91	1108.5
92	665.0
93	586.0
94	507.0
95	507.0
96	450.5
97	394.0
98	394.0
99	714.5
100	1035.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12426766666542041
2	0.05388515218699877
3	0.012805318039234747
4	0.0035985747774491803
5	1.4020421210840964E-4
6	2.336736868473494E-4
7	0.0
8	4.206126363252289E-4
9	1.8693894947787953E-4
10	5.608168484336386E-4
11	0.002009593706887205
12	0.0026171452926903134
13	0.014113890685579902
14	0.008225313777026698
15	0.024722676068449568
16	0.010047968534436024
17	0.021124101291000386
18	0.005421229534858506
19	0.00733735376700677
20	0.005514699009597446
21	0.006355924282247904
22	0.0069634758680510115
23	0.008786130625460338
24	0.013833482261363085
25	0.016544097028792337
26	0.01953512022043841
27	0.008412252726504579
28	0.005140821110641686
29	0.01168368434236747
30	0.0028508189795376625
31	0.005701637959075325
32	0.005748372696444795
33	0.007384088504376241
34	0.012151031716062168
35	0.016357158079314458
36	0.014955115958230361
37	0.009533886423371855
38	0.005701637959075325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2139736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.46926400850621
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55992151103926	35.112139411584906
2	14.099282172707339	13.103665312279247
3	4.6876698674375294	6.534977059640217
4	2.0010047248171343	3.71940867359183
5	1.0105119683826234	2.34788737212637
6	0.5896326196184708	1.6439876321446703
7	0.4031756791357804	1.3114693952898557
8	0.2521161421666169	0.9372521256917282
9	0.19235497868540102	0.8044734859094238
>10	1.0037763435749465	8.373821960819994
>50	0.0818099985171134	2.6731545524264186
>100	0.09149176072971059	9.274660184561345
>500	0.017019982065481856	5.593777123528891
>1k	0.010130941705643961	8.320755240684345
>5k	1.0130941705643962E-4	0.2485704697208597
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5280	0.24675941331080095	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4996	0.23348674789787152	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4557	0.21297019819267426	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3754	0.17544220408498992	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3587	0.16763750294428845	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3382	0.15805688178354713	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3274	0.15300953014764437	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3233	0.1510934059154961	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3112	0.14543850269379024	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	3000	0.14020421210840964	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2980	0.13926951736102025	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2907	0.13585788153304892	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2906	0.13581114679567946	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2895	0.1352970646846153	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2670	0.12478174877648457	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2629	0.12286562454433632	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2549	0.11912684555477873	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2476	0.11571520972680743	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2421	0.11314479917148657	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	2418	0.11300459495937817	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2389	0.11164928757566353	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2285	0.10678887488923869	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	2213	0.10342397379863684	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2166	0.10122744114227175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	9.346947473893975E-5	0.0	0.0	0.0
2	0.0	9.346947473893975E-5	0.0	0.0	0.0
3	0.0	9.346947473893975E-5	0.0	0.0	0.0
4	0.0	9.346947473893975E-5	0.0	4.6734737369469876E-5	0.0
5	0.0	9.346947473893975E-5	0.0	4.6734737369469876E-5	0.0
6	4.6734737369469876E-5	9.346947473893975E-5	0.0	4.6734737369469876E-5	0.0
7	4.6734737369469876E-5	9.346947473893975E-5	0.0	9.346947473893975E-5	0.0
8	4.6734737369469876E-5	9.346947473893975E-5	0.0	9.346947473893975E-5	0.0
9	4.6734737369469876E-5	9.346947473893975E-5	0.0	9.346947473893975E-5	0.0
10	4.6734737369469876E-5	9.346947473893975E-5	0.0	9.346947473893975E-5	4.6734737369469876E-5
11	4.6734737369469876E-5	9.346947473893975E-5	0.0	9.346947473893975E-5	4.6734737369469876E-5
12	4.6734737369469876E-5	9.346947473893975E-5	0.0	9.346947473893975E-5	1.4020421210840964E-4
13	4.6734737369469876E-5	9.346947473893975E-5	0.0	9.346947473893975E-5	1.4020421210840964E-4
14	4.6734737369469876E-5	9.346947473893975E-5	0.0	9.346947473893975E-5	1.4020421210840964E-4
15	4.6734737369469876E-5	9.346947473893975E-5	0.0	9.346947473893975E-5	2.336736868473494E-4
16	4.6734737369469876E-5	9.346947473893975E-5	0.0	1.4020421210840964E-4	2.336736868473494E-4
17	4.6734737369469876E-5	9.346947473893975E-5	0.0	1.4020421210840964E-4	2.336736868473494E-4
18	4.6734737369469876E-5	9.346947473893975E-5	0.0	1.869389494778795E-4	2.336736868473494E-4
19	4.6734737369469876E-5	9.346947473893975E-5	0.0	1.869389494778795E-4	2.336736868473494E-4
20	4.6734737369469876E-5	9.346947473893975E-5	0.0	1.869389494778795E-4	3.2714316158628917E-4
21	4.6734737369469876E-5	9.346947473893975E-5	0.0	2.336736868473494E-4	3.73877898955759E-4
22	4.6734737369469876E-5	9.346947473893975E-5	0.0	3.73877898955759E-4	3.73877898955759E-4
23	4.6734737369469876E-5	9.346947473893975E-5	0.0	5.608168484336386E-4	3.73877898955759E-4
24	4.6734737369469876E-5	9.346947473893975E-5	0.0	9.346947473893976E-4	3.73877898955759E-4
25	4.6734737369469876E-5	9.346947473893975E-5	0.0	0.0011216336968672771	3.73877898955759E-4
26	4.6734737369469876E-5	9.346947473893975E-5	0.0	0.001495511595823036	3.73877898955759E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	765	0.0	20.703976	28
CTAGCGG	780	0.0	20.307247	29
TAGCGGC	800	0.0	19.999561	30
CATCGTT	730	0.0	17.751791	28
CGGTCCA	930	0.0	17.376774	10
CGCCGGT	915	0.0	17.309881	7
CGGCATC	760	0.0	17.26278	25
TAGGACG	550	0.0	17.160051	4
AAGACGG	1035	0.0	17.001678	5
CGTTTAT	725	0.0	16.992731	31
ATCGTTT	745	0.0	16.966072	29
CAAGACG	1010	0.0	16.630188	4
GGACGTG	550	0.0	16.57913	6
TCGTTTA	745	0.0	16.53655	30
AGCGGCG	985	0.0	16.405731	31
GCCGGTC	1020	0.0	16.312225	8
CCGGTCC	1005	0.0	16.239208	9
GCATCGT	775	0.0	16.101744	27
GTCCTAT	710	0.0	16.01462	1
GTATCAA	4035	0.0	15.915399	1
>>END_MODULE