FastQCFastQC Report
Thu 2 Feb 2017
SRR4062802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139736
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT52570.24568451435130315No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT51570.24101104061435616No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC48600.22713082361562362No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT36740.17170342509543235No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC36240.16936668822695886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34070.1592252502177839No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA33890.15838402494513343No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC33540.15674830913720197No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG32740.15300953014764437No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC32090.14997177221862884No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC31060.14515809426957343No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG30110.14071829421947382No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG29720.13889563946206449No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG29680.1387087005125866No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG28210.1318386941192745No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG26430.12351991086750888No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC26210.12249174664538055No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA24270.11342520759570339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT23330.10903214228297321No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG23120.10805071279821436No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA22910.10706928331345548No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT22800.10655520120239133No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG22750.10632152751554398No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC21940.10253601378861692No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT21910.1023958095765085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG10650.019.9813524
CTAGCGG6950.019.79776629
TCTAGCG7000.019.65635328
AAGACGG11000.019.2014735
CGCAAGA10700.018.5413112
GCGCAAG11150.018.369551
TAGGACG6050.018.2480554
TAGCGGC7700.017.86941330
GCGAATT450.00885355417.7774813
GACGGAC11800.017.2224667
GCAAGAC12700.017.1335723
ATCGTTT6950.017.03528629
AGGACGT6500.016.9859185
CATCGTT7000.016.91360528
GTCCTAA3350.016.718091
AGAGCGA12350.016.4527815
GTAGGAC7450.016.3218733
GAGCGAA12400.016.25741216
TGTAGGA7700.016.2071422
CAGAGCG12550.016.19058614
Produced by FastQC (version 0.11.5)