##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062802_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2139736
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.239226240994217	32.0	32.0	32.0	32.0	32.0
2	31.336902776791156	32.0	32.0	32.0	32.0	32.0
3	31.404519062164677	32.0	32.0	32.0	32.0	32.0
4	31.496941211439168	32.0	32.0	32.0	32.0	32.0
5	31.422995173236323	32.0	32.0	32.0	32.0	32.0
6	34.96347680274576	36.0	36.0	36.0	36.0	36.0
7	34.987684462008396	36.0	36.0	36.0	36.0	36.0
8	34.920336901374746	36.0	36.0	36.0	36.0	36.0
9	35.0210708236904	36.0	36.0	36.0	36.0	36.0
10	34.88387212254222	36.0	36.0	36.0	32.0	36.0
11	35.04448819854412	36.0	36.0	36.0	36.0	36.0
12	34.93952431514916	36.0	36.0	36.0	32.0	36.0
13	35.002181110193035	36.0	36.0	36.0	36.0	36.0
14	34.93385866293786	36.0	36.0	36.0	32.0	36.0
15	34.90891680095115	36.0	36.0	36.0	32.0	36.0
16	34.920785554853495	36.0	36.0	36.0	32.0	36.0
17	34.87956037567251	36.0	36.0	36.0	32.0	36.0
18	34.88546344034965	36.0	36.0	36.0	32.0	36.0
19	34.88147743459941	36.0	36.0	36.0	32.0	36.0
20	34.866309208238775	36.0	36.0	36.0	32.0	36.0
21	34.85845309888697	36.0	36.0	36.0	32.0	36.0
22	34.842445049295804	36.0	36.0	36.0	32.0	36.0
23	34.78555672288544	36.0	36.0	36.0	32.0	36.0
24	34.75904317168099	36.0	36.0	36.0	32.0	36.0
25	34.73431161601244	36.0	36.0	36.0	32.0	36.0
26	34.675341724399644	36.0	36.0	36.0	32.0	36.0
27	34.66541807026661	36.0	36.0	36.0	32.0	36.0
28	34.64048134910101	36.0	36.0	36.0	32.0	36.0
29	34.60139615354417	36.0	36.0	36.0	32.0	36.0
30	34.57938736367477	36.0	36.0	36.0	32.0	36.0
31	34.58370799014458	36.0	36.0	36.0	32.0	36.0
32	34.55572276206037	36.0	36.0	36.0	32.0	36.0
33	34.526137803915994	36.0	36.0	36.0	32.0	36.0
34	34.51807606171976	36.0	36.0	36.0	32.0	36.0
35	34.49720292596844	36.0	36.0	36.0	32.0	36.0
36	34.466206111408134	36.0	36.0	36.0	32.0	36.0
37	34.46964111460479	36.0	36.0	36.0	32.0	36.0
38	34.02937044569984	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	42.0
22	143.0
23	521.0
24	1330.0
25	3344.0
26	7042.0
27	13691.0
28	23931.0
29	38170.0
30	57622.0
31	83246.0
32	119043.0
33	192899.0
34	475732.0
35	1122970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.357327778201025	18.1445583898841	11.878675760905585	26.619438071009288
2	16.39286341866473	19.57933128198993	36.199839606381346	27.82796569296399
3	18.914436173434478	22.77491241910217	27.9375586520954	30.373092755367953
4	12.298648755380933	15.149895010167166	35.0351983496991	37.516257884752804
5	14.676263498173137	36.209220398423355	32.631158826284015	16.48335727711949
6	35.91932455155641	34.92335963255246	15.5506245168792	13.606691299011933
7	31.577028770119455	29.77123174994859	19.464556110145253	19.187183369786702
8	28.9022562997208	32.68143448281019	18.279448782024378	20.13686043544463
9	27.083049994321534	14.095630947490328	18.32223111770405	40.49908794048409
10	16.596727315043946	25.773901241803216	29.903571089740623	27.72580035341221
11	39.11599710417357	20.71614217591725	21.045965402445564	19.12189531746362
12	25.58220071167991	23.408324243384435	27.046547081261807	23.962927963673852
13	29.433045555726395	18.607198755357565	25.130261701120375	26.82949398779566
14	23.786812952625933	19.658126049194856	23.7401716847312	32.81488931344801
15	25.420846310012074	27.32580093992904	21.370066213775903	25.88328653628298
16	26.683618913735152	25.576145842290828	22.88927232144526	24.850962922528762
17	24.523492617780885	25.967642737234875	24.168589022197132	25.34027562278711
18	25.790564817341956	24.10507651411202	25.749157840032606	24.355200828513425
19	25.92548800412761	24.376418399279164	24.863394362669037	24.834699233924184
20	26.468732591310328	23.61478238436891	24.292809954125183	25.623675070195574
21	27.645144345179677	23.53645222859685	23.854529445743673	24.963873980479796
22	26.483372941041765	23.882630325717905	24.65253874895723	24.9814579842831
23	24.415265283346365	23.65479585985341	25.575644788994506	26.354294067805718
24	25.069494554468402	24.56466592140339	25.099171112698016	25.266668411430192
25	25.164786683964753	24.056051774611447	25.07491578400326	25.70424575742054
26	24.718014146611615	25.664019142557372	25.373095266469914	24.244871444361102
27	25.73935557440524	24.157383975118414	24.753859706926328	25.34940074355002
28	24.651452328698493	24.321925695506362	25.66204428957591	25.364577686219235
29	24.464747052907462	24.404552711175583	25.65788489795003	25.472815337966924
30	24.244065623048826	24.823155753793927	25.983205404778907	24.949573218378344
31	25.506744757297163	24.521903636710324	24.29481954783207	25.676532058160443
32	25.235449606867387	24.413479046013155	24.269676259127294	26.081395087992163
33	24.400019441650745	24.140267771351233	25.278492299984674	26.181220487013352
34	25.624376091256117	24.299726695255863	25.261574325056923	24.814322888431096
35	26.143645758168297	24.049462176642354	25.362848500936565	24.444043564252787
36	24.293510975185722	25.00191612423215	25.0078047011407	25.69676819944143
37	25.96100640452841	24.697299106057944	24.516529141912834	24.825165347500814
38	24.704729929299692	24.169757390631368	25.209511827627335	25.916000852441613
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	40.0
2	66.0
3	66.0
4	233.0
5	400.0
6	400.0
7	653.0
8	906.0
9	1039.5
10	1173.0
11	1173.0
12	1564.0
13	1955.0
14	2890.0
15	3825.0
16	3825.0
17	5628.5
18	7432.0
19	7432.0
20	9037.5
21	10643.0
22	11454.5
23	12266.0
24	12266.0
25	14595.0
26	16924.0
27	16924.0
28	22418.5
29	27913.0
30	35318.5
31	42724.0
32	42724.0
33	56042.0
34	69360.0
35	69360.0
36	78871.5
37	88383.0
38	101589.5
39	114796.0
40	114796.0
41	120364.5
42	125933.0
43	144843.0
44	163753.0
45	163753.0
46	164875.5
47	165998.0
48	165998.0
49	178403.5
50	190809.0
51	199312.5
52	207816.0
53	207816.0
54	197312.0
55	186808.0
56	186808.0
57	182292.5
58	177777.0
59	159836.5
60	141896.0
61	141896.0
62	136930.5
63	131965.0
64	111176.0
65	90387.0
66	90387.0
67	76873.5
68	63360.0
69	63360.0
70	50696.5
71	38033.0
72	29850.0
73	21667.0
74	21667.0
75	16092.5
76	10518.0
77	10518.0
78	10521.5
79	10525.0
80	8175.5
81	5826.0
82	5826.0
83	5528.0
84	5230.0
85	5230.0
86	3336.5
87	1443.0
88	1185.0
89	927.0
90	927.0
91	559.0
92	191.0
93	119.0
94	47.0
95	47.0
96	33.0
97	19.0
98	19.0
99	23.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009954499059697084
2	0.0
3	0.0
4	2.336736868473494E-4
5	9.346947473893977E-5
6	9.346947473893977E-5
7	7.477557979115181E-4
8	0.0017759200200398555
9	0.003411635827971301
10	0.001168368434236747
11	0.004907147423794337
12	4.673473736946988E-4
13	2.336736868473494E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.8693894947787953E-4
22	4.673473736946988E-5
23	2.336736868473494E-4
24	0.0
25	0.0
26	4.673473736946988E-5
27	4.673473736946988E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2139736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.65861934967284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.88499192644107	32.996017300965825
2	14.998380818381682	13.396139596590842
3	5.019326880229424	6.724686256072402
4	2.1480909222732643	3.8372309930515742
5	1.1184143075778692	2.497341941867399
6	0.6413458477588803	1.7184972051934237
7	0.4185949040121385	1.3085709335993652
8	0.28094815275097623	1.003740528855967
9	0.20841868049110693	0.8376921465672085
>10	1.0683695155841428	8.525494281613174
>50	0.086400790030343	2.701814579836132
>100	0.09640829032941257	9.314043842590964
>500	0.018416905293275648	5.6848902695982435
>1k	0.011681579928877696	8.96439785582412
>5k	2.1047891763743595E-4	0.4894422677733456
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5257	0.24568451435130315	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5157	0.24101104061435616	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4860	0.22713082361562362	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3674	0.17170342509543235	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3624	0.16936668822695886	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3407	0.1592252502177839	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3389	0.15838402494513343	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3354	0.15674830913720197	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3274	0.15300953014764437	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3209	0.14997177221862884	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3106	0.14515809426957343	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3011	0.14071829421947382	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2972	0.13889563946206449	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2968	0.1387087005125866	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2821	0.1318386941192745	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2643	0.12351991086750888	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2621	0.12249174664538055	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2427	0.11342520759570339	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2333	0.10903214228297321	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	2312	0.10805071279821436	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2291	0.10706928331345548	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2280	0.10655520120239133	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2275	0.10632152751554398	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	2194	0.10253601378861692	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2191	0.1023958095765085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.346947473893975E-5
2	0.0	0.0	0.0	0.0	9.346947473893975E-5
3	0.0	0.0	0.0	0.0	9.346947473893975E-5
4	0.0	0.0	0.0	0.0	9.346947473893975E-5
5	0.0	0.0	0.0	0.0	9.346947473893975E-5
6	0.0	0.0	0.0	0.0	1.4020421210840964E-4
7	0.0	0.0	0.0	0.0	1.869389494778795E-4
8	0.0	0.0	0.0	0.0	1.869389494778795E-4
9	0.0	0.0	0.0	4.6734737369469876E-5	1.869389494778795E-4
10	0.0	0.0	0.0	4.6734737369469876E-5	2.336736868473494E-4
11	0.0	0.0	0.0	4.6734737369469876E-5	2.336736868473494E-4
12	0.0	0.0	0.0	4.6734737369469876E-5	3.2714316158628917E-4
13	0.0	0.0	0.0	4.6734737369469876E-5	3.2714316158628917E-4
14	0.0	0.0	0.0	4.6734737369469876E-5	3.2714316158628917E-4
15	0.0	0.0	0.0	4.6734737369469876E-5	3.73877898955759E-4
16	0.0	0.0	0.0	9.346947473893975E-5	4.206126363252289E-4
17	0.0	0.0	0.0	9.346947473893975E-5	4.206126363252289E-4
18	0.0	0.0	0.0	9.346947473893975E-5	4.673473736946988E-4
19	0.0	0.0	0.0	9.346947473893975E-5	4.673473736946988E-4
20	0.0	0.0	0.0	9.346947473893975E-5	4.673473736946988E-4
21	0.0	0.0	0.0	1.4020421210840964E-4	4.673473736946988E-4
22	0.0	0.0	0.0	1.4020421210840964E-4	5.140821110641686E-4
23	0.0	0.0	0.0	3.2714316158628917E-4	5.140821110641686E-4
24	0.0	0.0	0.0	6.075515858031084E-4	5.140821110641686E-4
25	0.0	0.0	0.0	8.412252726504578E-4	5.140821110641686E-4
26	0.0	0.0	0.0	0.0012618379089756866	5.140821110641686E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	1065	0.0	19.981352	4
CTAGCGG	695	0.0	19.797766	29
TCTAGCG	700	0.0	19.656353	28
AAGACGG	1100	0.0	19.201473	5
CGCAAGA	1070	0.0	18.541311	2
GCGCAAG	1115	0.0	18.36955	1
TAGGACG	605	0.0	18.248055	4
TAGCGGC	770	0.0	17.869413	30
GCGAATT	45	0.008853554	17.77748	13
GACGGAC	1180	0.0	17.222466	7
GCAAGAC	1270	0.0	17.133572	3
ATCGTTT	695	0.0	17.035286	29
AGGACGT	650	0.0	16.985918	5
CATCGTT	700	0.0	16.913605	28
GTCCTAA	335	0.0	16.71809	1
AGAGCGA	1235	0.0	16.45278	15
GTAGGAC	745	0.0	16.321873	3
GAGCGAA	1240	0.0	16.257412	16
TGTAGGA	770	0.0	16.207142	2
CAGAGCG	1255	0.0	16.190586	14
>>END_MODULE