FastQCFastQC Report
Thu 2 Feb 2017
SRR4062801_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062801_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999682
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC32680.1634259847315723No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32200.16102560307088828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30340.15172412413573758No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA26420.1321210072401512No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25140.12571998947832705No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24180.12091922615695895No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24140.12071919435190194No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23220.11611846283559085No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22470.11236786649077204No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22420.11211782673445078No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG22030.11016751663514499No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20360.10181618877401508No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20030.10016592638229478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5700.017.68328528
CTAGCGG6150.016.65036829
ATAACGA2750.016.29168712
ACGAACG2750.016.29168715
CGAACGA2750.016.29127916
CAAGACG7900.016.1984424
TAGCGGC6250.016.12836530
ATCGTTT5100.015.99996329
ATTACAC2200.015.9971633
ACGCGTA600.002446463615.9963626
ATACGCG600.00244687215.9959624
TAACGAA2950.015.72956413
CGCAAGA7950.015.7016112
TACCGTC3800.015.5769637
AAGACGG8150.015.5052885
CGGTCCA6850.015.41679310
ACCGTCG4050.015.4054448
CGCTTCG4100.015.21985632
GATAACG3050.015.213840511
CGATAAC2950.015.18716510
Produced by FastQC (version 0.11.5)