##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062801_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1999682
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297397786248013	32.0	32.0	32.0	32.0	32.0
2	30.931069039977356	32.0	32.0	32.0	32.0	32.0
3	30.98089996309413	32.0	32.0	32.0	32.0	32.0
4	31.044898638883584	32.0	32.0	32.0	32.0	32.0
5	30.95199586734291	32.0	32.0	32.0	32.0	32.0
6	34.62232094903089	36.0	36.0	36.0	32.0	36.0
7	34.57060422607194	36.0	36.0	36.0	32.0	36.0
8	34.55056153928474	36.0	36.0	36.0	32.0	36.0
9	34.68438481718593	36.0	36.0	36.0	32.0	36.0
10	34.41862906202086	36.0	36.0	36.0	32.0	36.0
11	34.6609075843059	36.0	36.0	36.0	32.0	36.0
12	34.488979247700385	36.0	36.0	36.0	32.0	36.0
13	34.56849539076713	36.0	36.0	36.0	32.0	36.0
14	34.47004623735174	36.0	36.0	36.0	32.0	36.0
15	34.408333425014575	36.0	36.0	36.0	32.0	36.0
16	34.43314036931872	36.0	36.0	36.0	32.0	36.0
17	34.355148968686024	36.0	36.0	36.0	32.0	36.0
18	34.38150565939984	36.0	36.0	36.0	32.0	36.0
19	34.373160332492866	36.0	36.0	36.0	32.0	36.0
20	34.35573206139776	36.0	36.0	36.0	32.0	36.0
21	34.34262147681481	36.0	36.0	36.0	32.0	36.0
22	34.31477704955088	36.0	36.0	36.0	32.0	36.0
23	34.25345629955163	36.0	36.0	36.0	32.0	36.0
24	34.235301913004164	36.0	36.0	36.0	32.0	36.0
25	34.2149836824055	36.0	36.0	36.0	32.0	36.0
26	34.15813314316976	36.0	36.0	36.0	32.0	36.0
27	34.16554132107005	36.0	36.0	36.0	32.0	36.0
28	34.144541482095654	36.0	36.0	36.0	32.0	36.0
29	34.11752168594806	36.0	36.0	36.0	32.0	36.0
30	34.09032986244813	36.0	36.0	36.0	32.0	36.0
31	34.10568480388382	36.0	36.0	36.0	32.0	36.0
32	34.06130124689826	36.0	36.0	36.0	32.0	36.0
33	34.02712681316329	36.0	36.0	36.0	32.0	36.0
34	34.04672392910473	36.0	36.0	36.0	32.0	36.0
35	33.99480267362511	36.0	36.0	36.0	32.0	36.0
36	33.96576805712108	36.0	36.0	36.0	32.0	36.0
37	33.95506085467589	36.0	36.0	36.0	32.0	36.0
38	33.358209455303395	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	11.0
10	16.0
11	17.0
12	8.0
13	12.0
14	134.0
15	312.0
16	531.0
17	607.0
18	798.0
19	1061.0
20	1439.0
21	2178.0
22	3403.0
23	5241.0
24	7902.0
25	12050.0
26	17586.0
27	25265.0
28	36230.0
29	49845.0
30	67855.0
31	91170.0
32	126099.0
33	192055.0
34	431803.0
35	926049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.21844111394494	16.618451528014347	11.027948758773103	26.135158599267612
2	17.122021389705424	18.97329413972106	37.055175433110264	26.849509037463253
3	19.70300624776187	22.628931358643136	27.64044460537872	30.027617788216276
4	12.760410675975056	14.558243275005875	35.252571725769265	37.428774323249804
5	15.046037458068206	36.00798329329351	33.31519706192403	15.630782186714248
6	35.70892055405031	34.474019265140214	16.315709460642083	13.501350720167387
7	31.490422722424228	29.53731120113658	20.090954507243904	18.881311569195287
8	28.79216433062156	32.596880694672535	18.869506860111336	19.741448114594565
9	27.311538124945304	14.289522047332692	18.165651918436744	40.23328790928526
10	16.172926619355504	26.015655591000307	30.949522145915147	26.86189564372904
11	38.51041261906512	20.82068731337275	21.433147249931363	19.235752817630765
12	25.307407177735058	23.093045306426994	28.00545901537686	23.594088500461087
13	29.536815486431767	18.724842150751034	25.15424812541513	26.584094237402066
14	23.72929842969643	19.3853820431851	24.285741435548935	32.59957809156953
15	25.54849165420341	26.8414023539298	21.73483525992767	25.875270731939114
16	26.516314342487412	25.383345411733405	23.163870629582227	24.93646961619696
17	24.644921461569087	25.640031691472316	24.60160578129283	25.11344106566577
18	25.76215479297706	24.169755261279853	25.61597270600897	24.45211723973412
19	25.97248145058524	24.68907600008802	24.73673734348775	24.60170520583899
20	26.060257196326	23.968099013683997	24.504816554825506	25.4668272351645
21	27.047439768827523	23.945685670647237	23.93718374070914	25.0696908198161
22	26.110490407207323	23.998735697831783	24.49025317050775	25.400520724453145
23	24.47532477275285	23.82850498255792	25.160604658722914	26.535565585966314
24	25.062181434246728	24.661859536228782	24.80950449411846	25.46645453540603
25	25.27396513452926	24.137941382714914	24.82971897754361	25.758374505212217
26	24.86206470542043	25.215937322282173	25.153615727849697	24.7683822444477
27	25.77421263396588	24.36674880456598	24.448319890691746	25.410718670776394
28	24.91863226183308	24.18397210591909	25.25895645250665	25.638439179741184
29	24.664037313738884	24.684942770062985	25.226033994872658	25.424985921325465
30	24.820591450601263	24.80603872924237	25.507519906222463	24.865849913933904
31	25.490870736997508	24.608476168995264	24.203286824744858	25.69736626926237
32	25.120778503726882	24.522387283398885	24.1488491283943	26.20798508447994
33	24.616217129956794	24.165055016171518	25.00541384534057	26.213314008531118
34	25.580381100709754	24.420494153551605	25.041974693648505	24.95715005209013
35	26.140128522669524	24.136863438661635	25.126742078370018	24.59626596029882
36	24.679025818978065	25.050840709779322	24.659069652522632	25.611063818719987
37	25.972604822463484	24.654309429070416	24.586941778746034	24.78614396972007
38	24.95153924171166	24.459379982356083	24.907129382722964	25.68195139320929
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	78.0
2	119.0
3	119.0
4	360.0
5	601.0
6	601.0
7	696.0
8	791.0
9	814.5
10	838.0
11	838.0
12	1144.0
13	1450.0
14	2019.5
15	2589.0
16	2589.0
17	3800.5
18	5012.0
19	5012.0
20	6488.0
21	7964.0
22	9159.5
23	10355.0
24	10355.0
25	12858.0
26	15361.0
27	15361.0
28	20135.0
29	24909.0
30	31484.5
31	38060.0
32	38060.0
33	50132.0
34	62204.0
35	62204.0
36	71098.5
37	79993.0
38	92318.5
39	104644.0
40	104644.0
41	112208.5
42	119773.0
43	136878.5
44	153984.0
45	153984.0
46	160862.0
47	167740.0
48	167740.0
49	178687.0
50	189634.0
51	193413.5
52	197193.0
53	197193.0
54	188237.0
55	179281.0
56	179281.0
57	174308.5
58	169336.0
59	152441.5
60	135547.0
61	135547.0
62	127837.5
63	120128.0
64	99881.5
65	79635.0
66	79635.0
67	66084.0
68	52533.0
69	52533.0
70	41844.5
71	31156.0
72	24354.5
73	17553.0
74	17553.0
75	13000.0
76	8447.0
77	8447.0
78	8287.5
79	8128.0
80	6363.0
81	4598.0
82	4598.0
83	4733.5
84	4869.0
85	4869.0
86	3226.0
87	1583.0
88	1405.5
89	1228.0
90	1228.0
91	910.5
92	593.0
93	511.0
94	429.0
95	429.0
96	391.5
97	354.0
98	354.0
99	693.5
100	1033.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11921895581397443
2	0.05285840448631332
3	0.01230195601100575
4	0.0026004134657410527
5	3.000477075855061E-4
6	4.000636101140081E-4
7	5.0007951264251016E-5
8	3.000477075855061E-4
9	3.500556588497571E-4
10	6.501033664352632E-4
11	0.0020503260018342917
12	0.002700429368269555
13	0.01530243308686081
14	0.009801558447793199
15	0.023603752996726478
16	0.0122519480597415
17	0.020303228213285913
18	0.006050962102974373
19	0.00790125629975166
20	0.006000954151710122
21	0.006801081371938138
22	0.008001272202280162
23	0.01035164591169996
24	0.014552313817897046
25	0.017002703429845344
26	0.01875298172409413
27	0.009951582301585953
28	0.0059009382491816195
29	0.010201622057907207
30	0.0028004452707980567
31	0.006150978005502875
32	0.007701224494694657
33	0.008951423276300932
34	0.013152091182498018
35	0.01730275113743085
36	0.015002385379275304
37	0.010301637960435709
38	0.006301001859295628
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1999682.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.02955177556105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51809967818376	40.85261532524726
2	12.765421746082891	13.283583433493856
3	3.8290631624177243	5.976733201827195
4	1.5952555525238443	3.320017254611625
5	0.8682512251896328	2.2587361037599165
6	0.5248691504189327	1.6385223982269956
7	0.3532259350801917	1.2864730954408063
8	0.24048238346138645	1.0009752497131628
9	0.18403565943821623	0.8617763584161174
>10	0.9530923827378046	8.878072034703937
>50	0.07798195729674276	2.8516057325786495
>100	0.07181002977431471	8.235051042246528
>500	0.012500193283790878	4.585628901435009
>1k	0.005910944110939872	4.970209868298987
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3268	0.1634259847315723	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3220	0.16102560307088828	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3034	0.15172412413573758	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2642	0.1321210072401512	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2514	0.12571998947832705	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2418	0.12091922615695895	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2414	0.12071919435190194	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2322	0.11611846283559085	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2247	0.11236786649077204	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2242	0.11211782673445078	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2203	0.11016751663514499	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2036	0.10181618877401508	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2003	0.10016592638229478	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0003180505700406E-4
2	0.0	0.0	0.0	0.0	2.0003180505700406E-4
3	0.0	0.0	0.0	5.0007951264251016E-5	2.0003180505700406E-4
4	0.0	0.0	0.0	5.0007951264251016E-5	2.0003180505700406E-4
5	0.0	0.0	0.0	5.0007951264251016E-5	2.0003180505700406E-4
6	0.0	0.0	0.0	5.0007951264251016E-5	2.0003180505700406E-4
7	0.0	0.0	0.0	5.0007951264251016E-5	3.000477075855061E-4
8	0.0	0.0	0.0	5.0007951264251016E-5	3.5005565884975714E-4
9	0.0	0.0	0.0	1.0001590252850203E-4	3.5005565884975714E-4
10	0.0	0.0	0.0	1.0001590252850203E-4	3.5005565884975714E-4
11	0.0	0.0	0.0	1.0001590252850203E-4	3.5005565884975714E-4
12	0.0	0.0	0.0	1.0001590252850203E-4	6.501033664352632E-4
13	0.0	0.0	0.0	1.0001590252850203E-4	7.001113176995143E-4
14	0.0	0.0	0.0	1.0001590252850203E-4	7.001113176995143E-4
15	5.0007951264251016E-5	0.0	0.0	1.5002385379275305E-4	8.001272202280162E-4
16	5.0007951264251016E-5	0.0	0.0	2.500397563212551E-4	8.501351714922672E-4
17	5.0007951264251016E-5	0.0	0.0	2.500397563212551E-4	8.501351714922672E-4
18	5.0007951264251016E-5	0.0	0.0	2.500397563212551E-4	9.501510740207693E-4
19	5.0007951264251016E-5	0.0	0.0	2.500397563212551E-4	9.501510740207693E-4
20	5.0007951264251016E-5	0.0	0.0	3.5005565884975714E-4	9.501510740207693E-4
21	5.0007951264251016E-5	0.0	0.0	4.5007156137825917E-4	9.501510740207693E-4
22	5.0007951264251016E-5	0.0	0.0	6.000954151710122E-4	0.0010001590252850204
23	5.0007951264251016E-5	0.0	0.0	0.0011501828790777733	0.0010001590252850204
24	5.0007951264251016E-5	0.0	0.0	0.0014502305866632794	0.0010001590252850204
25	5.0007951264251016E-5	0.0	0.0	0.0017002703429845345	0.0010001590252850204
26	5.0007951264251016E-5	0.0	0.0	0.0021003339530985426	0.0010501669765492713
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	570	0.0	17.683285	28
CTAGCGG	615	0.0	16.650368	29
ATAACGA	275	0.0	16.291687	12
ACGAACG	275	0.0	16.291687	15
CGAACGA	275	0.0	16.291279	16
CAAGACG	790	0.0	16.198442	4
TAGCGGC	625	0.0	16.128365	30
ATCGTTT	510	0.0	15.999963	29
ATTACAC	220	0.0	15.997163	3
ACGCGTA	60	0.0024464636	15.996362	6
ATACGCG	60	0.002446872	15.995962	4
TAACGAA	295	0.0	15.729564	13
CGCAAGA	795	0.0	15.701611	2
TACCGTC	380	0.0	15.576963	7
AAGACGG	815	0.0	15.505288	5
CGGTCCA	685	0.0	15.416793	10
ACCGTCG	405	0.0	15.405444	8
CGCTTCG	410	0.0	15.219856	32
GATAACG	305	0.0	15.2138405	11
CGATAAC	295	0.0	15.187165	10
>>END_MODULE