FastQCFastQC Report
Thu 2 Feb 2017
SRR4062801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999682
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC34990.1749778214736143No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT31620.15812514189756172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31210.15607481589572741No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA27200.13602162743876275No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26730.13367125372934296No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26680.13342121397302173No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25070.1253699338194773No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24170.12086921820569471No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24040.12021911483925944No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC22520.1126179062470933No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22340.11171776312433677No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21120.10561679307009815No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21040.10521672945998414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20810.10406654658090637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG7500.020.05259729
TCTAGCG7500.019.62594628
TAGCGGC8150.018.25699830
AGCGGCG8700.017.47062331
GATCGAA550.00136477417.45521411
CGGTCCA8850.016.81420510
TAAACGC3350.016.71580528
CGCCGGT8650.016.6488697
AAGACGG7600.016.422095
CAAGACG7700.016.4158024
GCGGCGC9100.016.35104832
AAACGCT3350.016.2382129
GTAAACG3350.016.2382127
ATCGTTT5150.016.15474729
AACGCTT3450.015.76753730
CGCTTCG3450.015.76753732
GCCGGTC9300.015.6572968
CCGGTCC9850.015.5948959
ACCGTCC1255.1373718E-815.3613568
ACTGCGC951.4322493E-515.1592348
Produced by FastQC (version 0.11.5)