##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062801_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1999682
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20705592189158	32.0	32.0	32.0	32.0	32.0
2	31.26713497446094	32.0	32.0	32.0	32.0	32.0
3	31.362593152311216	32.0	32.0	32.0	32.0	32.0
4	31.46696174691776	32.0	32.0	32.0	32.0	32.0
5	31.378639203633377	32.0	32.0	32.0	32.0	32.0
6	34.92364535961218	36.0	36.0	36.0	36.0	36.0
7	34.950134071317336	36.0	36.0	36.0	36.0	36.0
8	34.87163959069492	36.0	36.0	36.0	32.0	36.0
9	35.00068260853476	36.0	36.0	36.0	36.0	36.0
10	34.83420563869655	36.0	36.0	36.0	32.0	36.0
11	35.02082081050887	36.0	36.0	36.0	36.0	36.0
12	34.91344273739524	36.0	36.0	36.0	32.0	36.0
13	34.97107690122729	36.0	36.0	36.0	36.0	36.0
14	34.918275505805425	36.0	36.0	36.0	32.0	36.0
15	34.876787409198066	36.0	36.0	36.0	32.0	36.0
16	34.89269093785912	36.0	36.0	36.0	32.0	36.0
17	34.859487158458194	36.0	36.0	36.0	32.0	36.0
18	34.86060083553285	36.0	36.0	36.0	32.0	36.0
19	34.85439934949657	36.0	36.0	36.0	32.0	36.0
20	34.84343310586383	36.0	36.0	36.0	32.0	36.0
21	34.835799892182855	36.0	36.0	36.0	32.0	36.0
22	34.81613426534819	36.0	36.0	36.0	32.0	36.0
23	34.75941374678574	36.0	36.0	36.0	32.0	36.0
24	34.73071118307811	36.0	36.0	36.0	32.0	36.0
25	34.71350694760467	36.0	36.0	36.0	32.0	36.0
26	34.64801303407242	36.0	36.0	36.0	32.0	36.0
27	34.62672714961679	36.0	36.0	36.0	32.0	36.0
28	34.600490978065515	36.0	36.0	36.0	32.0	36.0
29	34.56183833229483	36.0	36.0	36.0	32.0	36.0
30	34.53773900050108	36.0	36.0	36.0	32.0	36.0
31	34.54406000554088	36.0	36.0	36.0	32.0	36.0
32	34.52362175585918	36.0	36.0	36.0	32.0	36.0
33	34.48936630924317	36.0	36.0	36.0	32.0	36.0
34	34.48380742538063	36.0	36.0	36.0	32.0	36.0
35	34.44426363791843	36.0	36.0	36.0	32.0	36.0
36	34.42127648296079	36.0	36.0	36.0	32.0	36.0
37	34.41591062979013	36.0	36.0	36.0	32.0	36.0
38	33.9745579547148	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	10.0
21	31.0
22	138.0
23	486.0
24	1172.0
25	3155.0
26	6717.0
27	12733.0
28	23018.0
29	36218.0
30	54812.0
31	80313.0
32	116201.0
33	188651.0
34	463116.0
35	1012908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.504136761438176	17.82650858134814	11.782880914383567	26.88647374283012
2	16.088758112539896	19.87696043670944	36.35137986939924	27.682901581351434
3	18.696022667604147	23.347812302156044	27.92764049483868	30.02852453540113
4	12.265637033199377	15.417247594110245	35.26046219487557	37.05665317781481
5	14.579418127482269	36.47920019283066	32.946938563231555	15.994443116455518
6	35.78782605216835	34.907835253640584	15.913546167386784	13.390792526804288
7	31.253016109085507	29.705867791780584	20.01956313988395	19.02155295924996
8	28.64641338478918	32.92562722445956	18.570914908311135	19.857044482440113
9	27.207537092344285	14.108893560925061	17.97955995287067	40.704009393859984
10	15.872982534048255	26.339001291218217	30.9386786366896	26.849337538043926
11	38.7006399879174	20.725016240999935	21.302240632856382	19.27210313822629
12	25.197944666008226	23.262480153024867	27.76171127808269	23.77786390288422
13	29.632305985110612	18.757410564395585	24.994861675298885	26.61542177519492
14	23.66722492237512	19.31158019704143	24.173055602368578	32.84813927821488
15	25.49705403159102	26.94468420478856	21.5655789270494	25.992682836571014
16	26.550284770420884	25.443558247540484	22.91970569305804	25.086451288980594
17	24.60156164830208	25.85641116937593	24.377776066394556	25.16425111592743
18	25.773648010033597	24.249755711158073	25.527858929569803	24.448737349238527
19	25.90041816648847	24.781390241048324	24.656070315180116	24.662121277283088
20	26.124603812006107	23.882797364781	24.441286164500156	25.55131265871273
21	27.122839654344695	24.009741558649385	23.815210433669385	25.052208353336535
22	26.006097972626634	24.07824047935646	24.475203795005303	25.440457753011604
23	24.48180381131553	23.70447827324113	25.1975694074593	26.61614850798404
24	25.076987240971317	24.735832997446593	24.75963678224838	25.42754297933371
25	25.27698167857773	24.1299987347982	24.807906861144353	25.785112725479713
26	24.96363169925603	25.106304455560664	25.101653713767348	24.82841013141596
27	25.89145557862037	24.31490254185797	24.338656354344874	25.45498552517679
28	24.86170301077871	24.21750058259263	25.292121447310123	25.62867495931853
29	24.758786647076885	24.567056161929745	25.211658653725944	25.462498537267425
30	24.75588618590356	24.76848818962215	25.463548704243976	25.012076920230314
31	25.411390411075363	24.566906138075954	24.255406609650933	25.76629684119775
32	25.23961309848266	24.498595276648988	24.210099405805526	26.051692219062833
33	24.64731892370887	24.13008668378272	25.025479051169135	26.197115341339273
34	25.538010543676442	24.318816691854007	25.139597195954156	25.003575568515394
35	26.189614148649635	24.035471639990757	25.080087733949696	24.694826477409908
36	24.819096236301572	24.901609355887587	24.623015059394444	25.6562793484164
37	26.104750655354202	24.598061091713582	24.481592573219142	24.815595679713073
38	25.086538759662787	24.175543911481924	24.89210784514738	25.84580948370791
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	41.5
2	57.0
3	57.0
4	281.0
5	505.0
6	505.0
7	627.0
8	749.0
9	773.0
10	797.0
11	797.0
12	1037.0
13	1277.0
14	1931.0
15	2585.0
16	2585.0
17	3886.0
18	5187.0
19	5187.0
20	6509.0
21	7831.0
22	9006.0
23	10181.0
24	10181.0
25	12641.0
26	15101.0
27	15101.0
28	19847.5
29	24594.0
30	31173.5
31	37753.0
32	37753.0
33	49924.5
34	62096.0
35	62096.0
36	70968.0
37	79840.0
38	92361.0
39	104882.0
40	104882.0
41	112337.5
42	119793.0
43	136959.0
44	154125.0
45	154125.0
46	161273.5
47	168422.0
48	168422.0
49	179301.5
50	190181.0
51	193614.5
52	197048.0
53	197048.0
54	188458.0
55	179868.0
56	179868.0
57	175175.5
58	170483.0
59	153669.5
60	136856.0
61	136856.0
62	128901.0
63	120946.0
64	99922.0
65	78898.0
66	78898.0
67	66088.5
68	53279.0
69	53279.0
70	42343.0
71	31407.0
72	24717.5
73	18028.0
74	18028.0
75	13206.0
76	8384.0
77	8384.0
78	8156.5
79	7929.0
80	6153.5
81	4378.0
82	4378.0
83	4252.5
84	4127.0
85	4127.0
86	2623.0
87	1119.0
88	917.5
89	716.0
90	716.0
91	431.5
92	147.0
93	94.0
94	41.0
95	41.0
96	29.0
97	17.0
98	17.0
99	23.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008551359666186923
2	0.0
3	0.0
4	3.000477075855061E-4
5	0.0
6	1.0001590252850203E-4
7	1.5002385379275305E-4
8	0.0019503100993057897
9	0.0039006201986115795
10	0.0010001590252850202
11	0.005650898492860365
12	3.500556588497571E-4
13	1.5002385379275305E-4
14	5.0007951264251016E-5
15	0.0
16	5.0007951264251016E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0001590252850203E-4
22	5.0007951264251016E-5
23	2.5003975632125505E-4
24	0.0
25	5.0007951264251016E-5
26	5.0007951264251016E-5
27	1.5002385379275305E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1999682.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.1887213198515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1725052921354	38.731893616617484
2	13.39661906097955	13.447183613594268
3	4.13506300313532	6.226005741131605
4	1.7574708993203476	3.5282086877495087
5	0.9311596431537218	2.3366855917267255
6	0.566981171277665	1.7073635999314631
7	0.3680477288134406	1.2930291425675433
8	0.2819532867221248	1.1320699946010324
9	0.19867026936981508	0.897390610554733
>10	1.012751746973584	9.092938311825915
>50	0.07979075473984314	2.761333874238543
>100	0.07967394955144975	8.80546758913578
>500	0.011970156591198329	4.206680573022026
>1k	0.007343037236617463	5.833749053303412
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3499	0.1749778214736143	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3162	0.15812514189756172	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3121	0.15607481589572741	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2720	0.13602162743876275	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2673	0.13367125372934296	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2668	0.13342121397302173	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2507	0.1253699338194773	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2417	0.12086921820569471	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2404	0.12021911483925944	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2252	0.1126179062470933	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2234	0.11171776312433677	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2112	0.10561679307009815	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2104	0.10521672945998414	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2081	0.10406654658090637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.0007951264251016E-5
2	0.0	0.0	0.0	0.0	5.0007951264251016E-5
3	0.0	0.0	0.0	0.0	5.0007951264251016E-5
4	0.0	0.0	0.0	0.0	5.0007951264251016E-5
5	0.0	0.0	0.0	0.0	5.0007951264251016E-5
6	0.0	0.0	0.0	0.0	1.0001590252850203E-4
7	0.0	0.0	0.0	0.0	1.0001590252850203E-4
8	0.0	0.0	0.0	0.0	1.0001590252850203E-4
9	0.0	0.0	0.0	5.0007951264251016E-5	1.0001590252850203E-4
10	0.0	0.0	0.0	5.0007951264251016E-5	1.0001590252850203E-4
11	0.0	0.0	0.0	5.0007951264251016E-5	1.0001590252850203E-4
12	0.0	0.0	0.0	5.0007951264251016E-5	3.5005565884975714E-4
13	0.0	0.0	0.0	5.0007951264251016E-5	6.000954151710122E-4
14	0.0	0.0	0.0	5.0007951264251016E-5	6.000954151710122E-4
15	0.0	0.0	0.0	1.0001590252850203E-4	7.501192689637653E-4
16	0.0	0.0	0.0	1.5002385379275305E-4	7.501192689637653E-4
17	0.0	0.0	0.0	1.5002385379275305E-4	7.501192689637653E-4
18	0.0	0.0	0.0	2.0003180505700406E-4	7.501192689637653E-4
19	0.0	0.0	0.0	2.0003180505700406E-4	7.501192689637653E-4
20	0.0	0.0	0.0	2.500397563212551E-4	8.001272202280162E-4
21	0.0	0.0	0.0	3.5005565884975714E-4	9.001431227565183E-4
22	0.0	0.0	0.0	5.000795126425102E-4	9.001431227565183E-4
23	0.0	0.0	0.0	0.0010501669765492713	9.001431227565183E-4
24	0.0	0.0	0.0	0.0014002226353990285	9.001431227565183E-4
25	0.0	0.0	0.0	0.0016502623917202836	9.001431227565183E-4
26	0.0	0.0	0.0	0.0021503419043627935	0.0010001590252850204
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	750	0.0	20.052597	29
TCTAGCG	750	0.0	19.625946	28
TAGCGGC	815	0.0	18.256998	30
AGCGGCG	870	0.0	17.470623	31
GATCGAA	55	0.001364774	17.455214	11
CGGTCCA	885	0.0	16.814205	10
TAAACGC	335	0.0	16.715805	28
CGCCGGT	865	0.0	16.648869	7
AAGACGG	760	0.0	16.42209	5
CAAGACG	770	0.0	16.415802	4
GCGGCGC	910	0.0	16.351048	32
AAACGCT	335	0.0	16.23821	29
GTAAACG	335	0.0	16.23821	27
ATCGTTT	515	0.0	16.154747	29
AACGCTT	345	0.0	15.767537	30
CGCTTCG	345	0.0	15.767537	32
GCCGGTC	930	0.0	15.657296	8
CCGGTCC	985	0.0	15.594895	9
ACCGTCC	125	5.1373718E-8	15.361356	8
ACTGCGC	95	1.4322493E-5	15.159234	8
>>END_MODULE