FastQCFastQC Report
Thu 2 Feb 2017
SRR4062800_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062800_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912260
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29560.3240304299212944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18970.20794510336965338No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17090.18733694341525442No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15030.16475566176309384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11210.12288163462170873No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10260.11246793677241138No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9610.10534277508605003No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9570.10490430359765855No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9320.1021638567952119No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9290.10183500317891829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA350.0020660322.8574949
TAATACT1006.838309E-817.593524
TCTAGCG2150.016.37234528
GTTATAT1102.0074367E-716.0072691
ACCCGTT1001.3134286E-616.00024830
ACGCTTC2500.016.00024631
CTCTATA703.6980043E-415.9984912
ATACTGC600.002446500415.9949866
AAACGCT2650.015.69835629
AACGCTT2650.015.69835630
CGCTTCG2550.015.68651632
TAGCGGC2250.015.64468630
TAACCCG1153.3419565E-715.30458428
TCTAATA1153.3461038E-715.3029052
AACCCGT1052.1832766E-615.23832929
GTAAACG2750.015.12750527
CGGTCCA2600.014.76945910
TTAGTCT650.00417090414.7637924
CTAGCGG2400.014.66689429
CAAGACG2850.014.5911164
Produced by FastQC (version 0.11.5)