FastQCFastQC Report
Thu 2 Feb 2017
SRR4062800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912260
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32830.3598754740972968No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21980.24094008287111132No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA18430.20202573827636858No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17170.18821388639203737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13770.15094380987876263No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.11882577335408766No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10420.11422182272597724No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9970.10928901848157324No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9730.10665818955122443No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9370.10271194615570121No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9280.10172538530682043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC509.658652E-725.604213
TAGTACA502.7932403E-522.4036834
TAAACGC1450.019.86098128
ATCGTTT2250.019.19894829
CGCTTCG1450.018.75759332
CATCGTT2350.018.38197128
ATAATAC802.7905135E-618.002963
AAACGCT1600.017.99901429
TCTAGCG1700.017.88137228
CGTCTTA450.00885171117.77680215
CTAGCGG1800.017.77680229
TCGTTTA2450.017.63168530
AACGCTT1550.017.54742630
CCTAGTA550.00136385217.4555022
CCGTTAA550.001364854317.45358816
ATTATCC854.973863E-616.9439643
ACACGCT1700.016.9421049
CGTTTAT2750.016.29001631
GTCTAAC908.559082E-616.003511
CATTCGG600.0024422815.99912410
Produced by FastQC (version 0.11.5)