##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062800_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	912260
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19316861421086	32.0	32.0	32.0	32.0	32.0
2	31.272113213338304	32.0	32.0	32.0	32.0	32.0
3	31.359701181680663	32.0	32.0	32.0	32.0	32.0
4	31.454163286782276	32.0	32.0	32.0	32.0	32.0
5	31.37603095608708	32.0	32.0	32.0	32.0	32.0
6	34.88667704382522	36.0	36.0	36.0	36.0	36.0
7	34.90926819108587	36.0	36.0	36.0	36.0	36.0
8	34.858779295376316	36.0	36.0	36.0	32.0	36.0
9	34.95679521189135	36.0	36.0	36.0	32.0	36.0
10	34.81473044965251	36.0	36.0	36.0	32.0	36.0
11	34.986328458991956	36.0	36.0	36.0	36.0	36.0
12	34.87906627496547	36.0	36.0	36.0	32.0	36.0
13	34.93854164382961	36.0	36.0	36.0	36.0	36.0
14	34.87861903404731	36.0	36.0	36.0	32.0	36.0
15	34.85302326091246	36.0	36.0	36.0	32.0	36.0
16	34.8628384451801	36.0	36.0	36.0	32.0	36.0
17	34.83535724464517	36.0	36.0	36.0	32.0	36.0
18	34.822181176419	36.0	36.0	36.0	32.0	36.0
19	34.81976300616052	36.0	36.0	36.0	32.0	36.0
20	34.808214763335016	36.0	36.0	36.0	32.0	36.0
21	34.786310920132415	36.0	36.0	36.0	32.0	36.0
22	34.77398548659374	36.0	36.0	36.0	32.0	36.0
23	34.708564444347004	36.0	36.0	36.0	32.0	36.0
24	34.68836077434065	36.0	36.0	36.0	32.0	36.0
25	34.67887882840418	36.0	36.0	36.0	32.0	36.0
26	34.60838795957293	36.0	36.0	36.0	32.0	36.0
27	34.59006533225177	36.0	36.0	36.0	32.0	36.0
28	34.55666476662355	36.0	36.0	36.0	32.0	36.0
29	34.528975292131626	36.0	36.0	36.0	32.0	36.0
30	34.49414969416614	36.0	36.0	36.0	32.0	36.0
31	34.491680003507774	36.0	36.0	36.0	32.0	36.0
32	34.462063446824374	36.0	36.0	36.0	32.0	36.0
33	34.413092758643366	36.0	36.0	36.0	32.0	36.0
34	34.40716243176288	36.0	36.0	36.0	32.0	36.0
35	34.36154824282551	36.0	36.0	36.0	32.0	36.0
36	34.32223269681889	36.0	36.0	36.0	32.0	36.0
37	34.318607633788616	36.0	36.0	36.0	32.0	36.0
38	33.88800342007761	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	24.0
22	77.0
23	205.0
24	637.0
25	1562.0
26	3342.0
27	6582.0
28	11416.0
29	17458.0
30	26885.0
31	38381.0
32	54542.0
33	85960.0
34	204771.0
35	460412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1592622029764	18.32575985967605	12.14569572724532	26.36928221010223
2	15.670751759366846	21.030517615592046	36.74555499528643	26.553175629754676
3	18.09517023655537	24.987832416197136	28.581983206541995	28.3350141407055
4	12.285998040028149	16.512982073053895	35.79963124466982	35.401388642248136
5	14.17819481288229	36.90570670642141	33.073137044263696	15.842961436432596
6	33.825081802785405	35.69977692640764	16.84068599240344	13.634455278403518
7	29.819590115055423	30.809663077031008	20.973937140451802	18.396809667461767
8	28.037621884711722	32.65803219565134	19.617203900310777	19.68714201932616
9	27.710128849649102	14.325397434407702	18.33229923286554	39.63217448307766
10	15.90751637712552	26.639795318816812	31.521252992607273	25.931435311450397
11	37.256320618101256	21.230019743195943	22.205778694230403	19.3078809444724
12	25.082624742808225	23.806887752026018	28.509400311535018	22.601087193630743
13	29.831330795311416	19.5722925178047	24.991038729132846	25.605337957751033
14	23.98910398351347	19.602525595773134	25.034639247582923	31.373731173130466
15	25.31273978909521	26.91568193278232	22.67325104685068	25.09832723127179
16	25.77499835573192	25.677876921053212	23.85241049700743	24.69471422620744
17	24.05871133229562	25.668888255541184	25.38453949531932	24.88786091684388
18	25.192269747659658	24.361914366518317	26.475895029925677	23.969920855896344
19	25.429373205007344	25.068182316444876	25.66077653300594	23.841667945541843
20	25.837480542827702	23.77907614057396	25.606954157805887	24.77648915879245
21	26.97383854128985	24.199732970278145	24.451525774506774	24.374902713925227
22	25.688895027503182	24.485507389356957	25.04554632571049	24.780051257429367
23	24.194664015372826	24.175042394758506	25.566094716522393	26.064198873346278
24	24.63672637186767	25.02466402122202	25.4573257623923	24.88128384451801
25	24.9986023705987	24.226124379151095	25.345324080113212	25.42994917013699
26	24.504663697480648	25.067771323713988	25.82292967238471	24.604635306420654
27	25.488704413987694	24.658457740619713	25.034447454067323	24.81839039132527
28	24.58213667156293	24.376822397123625	25.948633065134942	25.0924078661785
29	24.533356718479382	24.783504702606713	25.997741871834783	24.68539670707912
30	24.631793567623266	24.775173744327276	26.086532348234055	24.506500339815403
31	24.974349417929098	24.61918751233201	24.91471729550786	25.491745774231028
32	24.540372262293644	24.97884375068511	24.918005831670794	25.562778155350447
33	24.27180847565387	24.438537259114725	25.579988161269814	25.70966610396159
34	24.97741871834784	24.630807006774386	25.799881612698133	24.591892662179642
35	25.853703987898186	24.42801394339333	25.523206103523115	24.195075965185364
36	24.73373818867428	25.23600727862671	25.205971981671894	24.824282551027117
37	25.549295157082412	25.014579176989017	25.084734615131648	24.351391050796924
38	24.622722275143353	24.825405942829832	25.34905109185001	25.202820690176804
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	214.0
1	177.0
2	140.0
3	140.0
4	476.5
5	813.0
6	813.0
7	960.0
8	1107.0
9	1084.5
10	1062.0
11	1062.0
12	1349.5
13	1637.0
14	2035.0
15	2433.0
16	2433.0
17	3517.5
18	4602.0
19	4602.0
20	5456.5
21	6311.0
22	6316.5
23	6322.0
24	6322.0
25	7204.5
26	8087.0
27	8087.0
28	10450.0
29	12813.0
30	16346.0
31	19879.0
32	19879.0
33	25052.5
34	30226.0
35	30226.0
36	33780.5
37	37335.0
38	42582.5
39	47830.0
40	47830.0
41	52160.5
42	56491.0
43	64559.5
44	72628.0
45	72628.0
46	78237.5
47	83847.0
48	83847.0
49	86404.0
50	88961.0
51	87512.5
52	86064.0
53	86064.0
54	82095.5
55	78127.0
56	78127.0
57	75681.5
58	73236.0
59	65182.5
60	57129.0
61	57129.0
62	53732.0
63	50335.0
64	41161.0
65	31987.0
66	31987.0
67	26607.5
68	21228.0
69	21228.0
70	16925.0
71	12622.0
72	9924.0
73	7226.0
74	7226.0
75	5473.5
76	3721.0
77	3721.0
78	3574.0
79	3427.0
80	2623.5
81	1820.0
82	1820.0
83	1786.0
84	1752.0
85	1752.0
86	1103.0
87	454.0
88	371.5
89	289.0
90	289.0
91	172.0
92	55.0
93	39.5
94	24.0
95	24.0
96	19.5
97	15.0
98	15.0
99	13.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009317519128318682
2	0.0
3	0.0
4	2.1923574419573365E-4
5	0.0
6	5.480893604893341E-4
7	4.384714883914673E-4
8	0.002740446802446671
9	0.003727007651327473
10	0.001315414465174402
11	0.005152039988599741
12	3.288536162936005E-4
13	1.0961787209786683E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.1923574419573365E-4
22	2.1923574419573365E-4
23	1.0961787209786683E-4
24	0.0
25	1.0961787209786683E-4
26	1.0961787209786683E-4
27	1.0961787209786683E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0961787209786683E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	912260.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.439914125149116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.78432613547331	50.03477562536345
2	11.198252539127838	13.53642843633239
3	2.7597839502982273	5.00403314879969
4	1.073792674284532	2.5959974808788533
5	0.5659540695392331	1.7103107680864962
6	0.33800131890194585	1.225726241317245
7	0.22479501032974325	0.9510613784064185
8	0.16001879671366698	0.7737217865426981
9	0.1181052382482074	0.642444341170674
>10	0.6234003926249535	6.94400464381172
>50	0.06821512573888584	2.932963953439519
>100	0.07824185305432249	9.79494960705002
>500	0.005836222084169399	2.4708388033973994
>1k	0.001276673580912056	1.3827437854033895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3283	0.3598754740972968	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2198	0.24094008287111132	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1843	0.20202573827636858	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1717	0.18821388639203737	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1377	0.15094380987876263	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1084	0.11882577335408766	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1042	0.11422182272597724	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	997	0.10928901848157324	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	973	0.10665818955122443	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	937	0.10271194615570121	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	928	0.10172538530682043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.0961787209786684E-4
11	0.0	0.0	0.0	0.0	1.0961787209786684E-4
12	0.0	0.0	0.0	0.0	3.288536162936005E-4
13	0.0	0.0	0.0	0.0	4.3847148839146736E-4
14	0.0	0.0	0.0	0.0	4.3847148839146736E-4
15	0.0	0.0	0.0	0.0	5.480893604893342E-4
16	0.0	0.0	0.0	0.0	6.57707232587201E-4
17	0.0	0.0	0.0	0.0	6.57707232587201E-4
18	0.0	0.0	0.0	0.0	7.673251046850678E-4
19	0.0	0.0	0.0	1.0961787209786684E-4	7.673251046850678E-4
20	0.0	0.0	0.0	1.0961787209786684E-4	7.673251046850678E-4
21	0.0	0.0	0.0	4.3847148839146736E-4	7.673251046850678E-4
22	0.0	0.0	0.0	7.673251046850678E-4	7.673251046850678E-4
23	0.0	0.0	0.0	0.0015346502093701356	8.769429767829347E-4
24	0.0	0.0	0.0	0.0023019753140552037	8.769429767829347E-4
25	0.0	0.0	0.0	0.002740446802446671	8.769429767829347E-4
26	0.0	0.0	0.0	0.0037270076513274725	8.769429767829347E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATAC	50	9.658652E-7	25.60421	3
TAGTACA	50	2.7932403E-5	22.403683	4
TAAACGC	145	0.0	19.860981	28
ATCGTTT	225	0.0	19.198948	29
CGCTTCG	145	0.0	18.757593	32
CATCGTT	235	0.0	18.381971	28
ATAATAC	80	2.7905135E-6	18.00296	3
AAACGCT	160	0.0	17.999014	29
TCTAGCG	170	0.0	17.881372	28
CGTCTTA	45	0.008851711	17.776802	15
CTAGCGG	180	0.0	17.776802	29
TCGTTTA	245	0.0	17.631685	30
AACGCTT	155	0.0	17.547426	30
CCTAGTA	55	0.001363852	17.455502	2
CCGTTAA	55	0.0013648543	17.453588	16
ATTATCC	85	4.973863E-6	16.943964	3
ACACGCT	170	0.0	16.942104	9
CGTTTAT	275	0.0	16.290016	31
GTCTAAC	90	8.559082E-6	16.00351	1
CATTCGG	60	0.00244228	15.999124	10
>>END_MODULE