FastQCFastQC Report
Thu 2 Feb 2017
SRR4062799_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062799_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502827
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT76510.3056943208619693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT48610.19422037559927235No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG32720.13073216806435284No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32410.12949356867254508No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC31730.12677664097438618No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG31290.125018628934401No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG31020.12393984881895552No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA31020.12393984881895552No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC30520.12194210786442691No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC29920.11954481871899257No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA29380.11738725848810165No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG27380.10939629466998717No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG26770.10695905070546227No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA26600.10627981878092255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT26060.10412225855003163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6450.015.87528528
CGTTATT3950.015.3975882
TAGGACG7200.015.3314484
TAACGAA3150.015.23804813
ATACCGT6200.015.223636
GTTATTC4350.015.0795093
ACCGTCG6350.014.8652048
CTAGCGG7000.014.62794229
GTCCTAT9800.014.3796571
TAGCGGC7150.014.32106130
GTATAGG2350.014.31012251
TACCGTC6500.014.2760237
GGACGTG7350.014.1476366
ACGAACG3400.014.11760215
TAACACC1258.613206E-714.0782694
CGTCGTA6600.014.06028110
AGGACGT7700.013.9200885
CGAACGA3450.013.91316
TACACCG1155.6284425E-613.9110555
CGGTCCA7650.013.80360310
Produced by FastQC (version 0.11.5)