##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062799_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2502827
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230346723924587	32.0	32.0	32.0	32.0	32.0
2	30.91769227357704	32.0	32.0	32.0	32.0	32.0
3	30.96801736596257	32.0	32.0	32.0	32.0	32.0
4	31.015136084116083	32.0	32.0	32.0	32.0	32.0
5	30.985788869945864	32.0	32.0	32.0	32.0	32.0
6	34.597143550073575	36.0	36.0	36.0	32.0	36.0
7	34.526749152058855	36.0	36.0	36.0	32.0	36.0
8	34.52629806215132	36.0	36.0	36.0	32.0	36.0
9	34.61206467726295	36.0	36.0	36.0	32.0	36.0
10	34.392834982202125	36.0	36.0	36.0	32.0	36.0
11	34.60383837956039	36.0	36.0	36.0	32.0	36.0
12	34.45357789411733	36.0	36.0	36.0	32.0	36.0
13	34.519295580557504	36.0	36.0	36.0	32.0	36.0
14	34.430550333682675	36.0	36.0	36.0	32.0	36.0
15	34.39632663384245	36.0	36.0	36.0	32.0	36.0
16	34.41101202759919	36.0	36.0	36.0	32.0	36.0
17	34.34498349266649	36.0	36.0	36.0	32.0	36.0
18	34.36728347584551	36.0	36.0	36.0	32.0	36.0
19	34.344388565410235	36.0	36.0	36.0	32.0	36.0
20	34.323310001050814	36.0	36.0	36.0	32.0	36.0
21	34.29961998971563	36.0	36.0	36.0	32.0	36.0
22	34.282003110882215	36.0	36.0	36.0	32.0	36.0
23	34.23607424724122	36.0	36.0	36.0	32.0	36.0
24	34.21886970214082	36.0	36.0	36.0	32.0	36.0
25	34.19716224892891	36.0	36.0	36.0	32.0	36.0
26	34.15711553375443	36.0	36.0	36.0	32.0	36.0
27	34.15879004022251	36.0	36.0	36.0	32.0	36.0
28	34.1392489373017	36.0	36.0	36.0	32.0	36.0
29	34.10448305056642	36.0	36.0	36.0	32.0	36.0
30	34.07804614541876	36.0	36.0	36.0	32.0	36.0
31	34.09573414383016	36.0	36.0	36.0	32.0	36.0
32	34.05266684433243	36.0	36.0	36.0	32.0	36.0
33	34.00900981170492	36.0	36.0	36.0	32.0	36.0
34	34.009347030378045	36.0	36.0	36.0	32.0	36.0
35	33.96849083056879	36.0	36.0	36.0	32.0	36.0
36	33.93476976235273	36.0	36.0	36.0	32.0	36.0
37	33.929689507105365	36.0	36.0	36.0	32.0	36.0
38	33.3099938589443	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	3.0
8	3.0
9	8.0
10	16.0
11	23.0
12	12.0
13	15.0
14	441.0
15	846.0
16	1046.0
17	1301.0
18	1663.0
19	2044.0
20	2544.0
21	3619.0
22	4873.0
23	7305.0
24	10551.0
25	15267.0
26	22474.0
27	32326.0
28	45342.0
29	61761.0
30	83234.0
31	112532.0
32	154071.0
33	232867.0
34	531746.0
35	1174892.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89820246698255	17.83527981070599	11.796992977480333	25.46952474483112
2	16.14364045432522	20.259983337784053	38.36583158369405	25.23054462419667
3	18.67213102307633	24.67851418629454	28.794763360627147	27.854591430001985
4	12.285611427717512	15.891634705247053	36.88883898836363	34.9339148786718
5	14.309904152078118	36.73997200756427	33.75206117581815	15.198062664539465
6	32.99198463171698	36.387758273976885	17.360638033064344	13.259619061241793
7	29.465464025651016	31.07220840450291	21.246111893560276	18.216215676285795
8	27.522914152835604	33.06170639518623	20.111634076761415	19.303745375216756
9	27.551812576139145	14.632540347504156	19.076729080289066	38.738917996067634
10	15.745950060232177	26.712521700565162	31.682325701260382	25.859202537942277
11	36.90566613698921	21.596416146678436	22.82265208182418	18.675265634508172
12	24.98213968920662	24.0562404005824	28.966906881853443	21.994713028357538
13	29.08568295651715	19.820032943609842	25.683028446431464	25.41125565344154
14	23.477959269462005	20.528259877551054	25.599318147490163	30.394462705496778
15	24.888389422702257	27.612482800321477	22.924538820818416	24.574588956157857
16	25.25633792011048	26.22378879833259	24.451360928586613	24.068512352970323
17	23.440178779094165	26.13329464901575	25.984831591340146	24.44169498054994
18	24.47015096531977	25.034103250337438	27.23565086263043	23.260094921712362
19	25.255989879479174	25.16216887939846	26.090509294592344	23.49133194653002
20	25.32699723538247	24.67322252753729	25.72728540450562	24.27249483257462
21	26.792284406426408	24.525866753613737	24.776398984765084	23.905449855194767
22	25.247787246403913	24.800741293931914	25.69635158877526	24.255119870888908
23	23.717801947337104	24.60516015962618	26.185638358872804	25.49139953416391
24	24.1872384569399	25.658513076753398	25.888087542797955	24.266160923508746
25	24.47241331769492	24.92310335939514	25.979735332492805	24.62474799041714
26	23.75621766285811	26.08183437388979	26.532451334686996	23.629496628565104
27	24.68803656332551	25.22103861677596	25.7043327045249	24.386592115373627
28	23.58903255709879	25.473812073457253	26.429187910559882	24.507967458884075
29	23.784790971459945	25.308435026151294	26.26473844909578	24.642035553292978
30	23.715429707087726	25.693403650285706	26.569874974178536	24.02129166844803
31	24.501845018450187	25.370312284606335	25.281487682229287	24.8463550147142
32	23.960173861759284	25.650762902778546	25.30485344099401	25.084209794468165
33	23.673698372543623	25.014854450361362	26.219433497015327	25.092013680079695
34	24.376040698062397	25.468016928488684	26.309046815478915	23.846895557970004
35	25.224101635366498	25.264382385803273	26.045141614011307	23.466374364818922
36	23.797223428822843	26.03140376248931	25.60178290060217	24.56958990808567
37	24.81816808693088	25.75404002100228	25.459784399017654	23.96800749304919
38	23.897511426755088	25.67928208132166	25.965015888973376	24.458190602949877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1226.0
1	896.0
2	566.0
3	566.0
4	1279.5
5	1993.0
6	1993.0
7	2340.0
8	2687.0
9	2517.0
10	2347.0
11	2347.0
12	3155.0
13	3963.0
14	5307.0
15	6651.0
16	6651.0
17	9672.0
18	12693.0
19	12693.0
20	15856.0
21	19019.0
22	20662.5
23	22306.0
24	22306.0
25	27173.5
26	32041.0
27	32041.0
28	41653.0
29	51265.0
30	62170.0
31	73075.0
32	73075.0
33	90639.0
34	108203.0
35	108203.0
36	116752.5
37	125302.0
38	139592.0
39	153882.0
40	153882.0
41	159529.0
42	165176.0
43	181633.5
44	198091.0
45	198091.0
46	200911.5
47	203732.0
48	203732.0
49	212686.0
50	221640.0
51	220248.0
52	218856.0
53	218856.0
54	206450.5
55	194045.0
56	194045.0
57	187423.5
58	180802.0
59	160235.5
60	139669.0
61	139669.0
62	134097.5
63	128526.0
64	106537.5
65	84549.0
66	84549.0
67	70639.5
68	56730.0
69	56730.0
70	45990.5
71	35251.0
72	27758.0
73	20265.0
74	20265.0
75	15344.0
76	10423.0
77	10423.0
78	10426.5
79	10430.0
80	8038.0
81	5646.0
82	5646.0
83	5811.5
84	5977.0
85	5977.0
86	3928.5
87	1880.0
88	1624.0
89	1368.0
90	1368.0
91	998.0
92	628.0
93	524.5
94	421.0
95	421.0
96	381.5
97	342.0
98	342.0
99	751.5
100	1161.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12034391510080403
2	0.05429859914408787
3	0.012226174641715147
4	0.0027968373363400664
5	3.1963855272457907E-4
6	3.995481909057238E-4
7	7.990963818114477E-5
8	2.7968373363400665E-4
9	3.1963855272457907E-4
10	4.7945782908686863E-4
11	0.0018379216781663293
12	0.002836792155430639
13	0.014743328244421209
14	0.009309472848103365
15	0.023972891454343427
16	0.010827755973545116
17	0.02081646074618821
18	0.005313990939046127
19	0.0075514608081181794
20	0.00519412648177441
21	0.006192996959038719
22	0.0070320481599407385
23	0.009189608390831648
24	0.014823237882602354
25	0.01766003003803299
26	0.019178313163474743
27	0.0083905120090202
28	0.00587335840631414
29	0.010947620430816832
30	0.0025571084217966326
31	0.005673584310861278
32	0.005953268044495285
33	0.0083905120090202
34	0.011986445727171714
35	0.015622334264413801
36	0.015502469807142084
37	0.009629111400827943
38	0.005913313225404712
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2502827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.15119365342735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.47947949774529	37.590577073070044
2	13.956224214474618	13.71930158072588
3	4.4521584524662075	6.5648670681873
4	1.8504841245109254	3.6381401422571784
5	0.9411270253683376	2.3128758338176594
6	0.5263338716303868	1.5521962830517988
7	0.33938395618748884	1.1676788587406295
8	0.23482962176628064	0.9233724971996445
9	0.17801982494847962	0.7874898201172764
>10	0.8589229231593243	7.725315447553154
>50	0.07484986050005245	2.60564528423572
>100	0.08490717486987256	9.037215464297658
>500	0.014866176603041572	5.085725106171092
>1k	0.008331593480825286	6.982428569291868
>5k	8.168228902769889E-5	0.3071709712833179
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7651	0.3056943208619693	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4861	0.19422037559927235	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3272	0.13073216806435284	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3241	0.12949356867254508	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3173	0.12677664097438618	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3129	0.125018628934401	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3102	0.12393984881895552	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3102	0.12393984881895552	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3052	0.12194210786442691	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2992	0.11954481871899257	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2938	0.11738725848810165	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2738	0.10939629466998717	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2677	0.10695905070546227	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2660	0.10627981878092255	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2606	0.10412225855003163	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.997740954528619E-4
2	0.0	0.0	0.0	0.0	1.997740954528619E-4
3	0.0	0.0	0.0	0.0	1.997740954528619E-4
4	0.0	0.0	0.0	3.9954819090572384E-5	1.997740954528619E-4
5	0.0	0.0	0.0	3.9954819090572384E-5	1.997740954528619E-4
6	0.0	0.0	0.0	3.9954819090572384E-5	2.397289145434343E-4
7	0.0	0.0	0.0	7.990963818114477E-5	2.397289145434343E-4
8	3.9954819090572384E-5	0.0	0.0	1.1986445727171714E-4	2.397289145434343E-4
9	3.9954819090572384E-5	0.0	0.0	1.1986445727171714E-4	2.397289145434343E-4
10	3.9954819090572384E-5	0.0	0.0	1.1986445727171714E-4	2.397289145434343E-4
11	3.9954819090572384E-5	0.0	0.0	1.1986445727171714E-4	2.397289145434343E-4
12	3.9954819090572384E-5	0.0	0.0	1.1986445727171714E-4	3.5959337181515143E-4
13	3.9954819090572384E-5	0.0	0.0	1.1986445727171714E-4	3.5959337181515143E-4
14	3.9954819090572384E-5	0.0	0.0	1.5981927636228953E-4	3.995481909057238E-4
15	3.9954819090572384E-5	0.0	0.0	1.5981927636228953E-4	6.792319245397304E-4
16	3.9954819090572384E-5	0.0	0.0	1.5981927636228953E-4	6.792319245397304E-4
17	3.9954819090572384E-5	0.0	0.0	1.5981927636228953E-4	6.792319245397304E-4
18	3.9954819090572384E-5	0.0	0.0	2.397289145434343E-4	7.591415627208753E-4
19	3.9954819090572384E-5	0.0	0.0	3.1963855272457907E-4	7.591415627208753E-4
20	3.9954819090572384E-5	0.0	0.0	3.5959337181515143E-4	7.990963818114476E-4
21	3.9954819090572384E-5	0.0	0.0	5.993222863585857E-4	8.3905120090202E-4
22	3.9954819090572384E-5	0.0	0.0	8.3905120090202E-4	8.3905120090202E-4
23	3.9954819090572384E-5	0.0	0.0	0.0015981927636228952	8.790060199925924E-4
24	3.9954819090572384E-5	0.0	0.0	0.002716927698158922	9.189608390831647E-4
25	3.9954819090572384E-5	0.0	0.0	0.0032762951654269354	9.189608390831647E-4
26	3.9954819090572384E-5	0.0	0.0	0.004474939738144106	9.189608390831647E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	645	0.0	15.875285	28
CGTTATT	395	0.0	15.397588	2
TAGGACG	720	0.0	15.331448	4
TAACGAA	315	0.0	15.238048	13
ATACCGT	620	0.0	15.22363	6
GTTATTC	435	0.0	15.079509	3
ACCGTCG	635	0.0	14.865204	8
CTAGCGG	700	0.0	14.627942	29
GTCCTAT	980	0.0	14.379657	1
TAGCGGC	715	0.0	14.321061	30
GTATAGG	235	0.0	14.3101225	1
TACCGTC	650	0.0	14.276023	7
GGACGTG	735	0.0	14.147636	6
ACGAACG	340	0.0	14.117602	15
TAACACC	125	8.613206E-7	14.078269	4
CGTCGTA	660	0.0	14.060281	10
AGGACGT	770	0.0	13.920088	5
CGAACGA	345	0.0	13.913	16
TACACCG	115	5.6284425E-6	13.911055	5
CGGTCCA	765	0.0	13.803603	10
>>END_MODULE