FastQCFastQC Report
Thu 2 Feb 2017
SRR4062799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502827
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT83690.3343818809690003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT56960.2275826495399003No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG34460.13768430658611241No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC33610.1342881469634138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT33380.1333691861243306No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA33330.13316941202887775No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT33200.13264999938070032No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC32290.1290141108434582No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG31440.12561795122075955No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA30500.12186219822624576No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA30390.12142269521624946No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC29350.11726739403082993No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG27300.1090766561172626No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG27280.10899674647908146No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG26500.10588027059001681No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG26300.10508117420820536No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.10404234891185048No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC26020.10396243927366933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG7000.017.59941729
TACCGTC6900.017.1612517
TCTAGCG7000.017.14228828
ACCGTCG7050.016.796128
TAGCGGC7400.016.64809830
ATACCGT7000.016.4588996
CGTCGTA7550.015.47092210
GAGCGTT1451.2205419E-915.4499257
ATCGTTT5600.015.42806129
GATATAC7400.015.1358431
CGGTCCA8500.015.05952710
CATCGTT5850.015.04223728
CCGTCGT7600.014.9486659
CGCCGGT8150.014.9218387
GTCGTAG7700.014.75393911
AGGGGCG7050.014.75128532
CGCTTCG7650.014.640038532
AGCGGCG8350.014.56239231
GCACCGT1002.3255116E-514.4015376
GTATCAA64700.014.170681
Produced by FastQC (version 0.11.5)