FastQCFastQC Report
Thu 2 Feb 2017
SRR4062798_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062798_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254854
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40270.3209138274253419No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24970.19898729254558697No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17260.13754588183167127No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA16500.13148940036052004No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14460.11523252904321937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14310.11403717085812373No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC14050.11196521667062463No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA13020.10375709046630124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12900.10280080391822474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG1051.2732926E-1121.3307047
TCTAGCG2500.020.47910728
CTAGCGG2500.019.83992429
CAAGACG3450.019.0098764
CGTCTTA1600.019.00068715
CAATACG1105.2204996E-1018.9045014
AGAACCG1450.018.7540655
AAGACGG3350.018.6223435
TGTAGGA14850.018.215222
AGGACCT10500.018.1289315
ACGTTTT7450.017.82543629
GTAGGAC15650.017.684723
CGCAAGA3650.017.5404742
TAGGACC10700.017.4910814
TAGCGGC2900.017.10338430
GTCCTAA5900.017.1003571
CACGTTT7600.017.0518928
CTGTAGG14850.016.823441
AGCGGCG3050.016.78682331
CGTTTTT7700.016.62331630
Produced by FastQC (version 0.11.5)